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Development and validation of a forensic six-dye multiplex assay with 29 STR loci.
Electrophoresis. 2021 08; 42(14-15):1419-1430.E

Abstract

This paper describes the development and validation of a novel 31-locus, six-dye STR multiplex system, which is designed to meet the needs of the rapidly growing Chinese forensic database. This new assay combines 20 extended-CODIS core loci (D3S1358, D5S818, TPOX, CSF1PO, TH01, vWA, D7S820, D21S11, D8S1179, D18S51, D16S539, D13S317, FGA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433, and D22S1045), nine highly polymorphic loci in Chinese Han population (D3S3045, D6S1043, D6S477, D8S1132, D10S1435, D15S659, D19S253, Penta D, and Penta E), and two gender determining markers, amelogenin and Y-Indel, which could amplify DNA from extracts, as well as direct amplification from substrates. To demonstrate the suitability for forensic applications, this system was validated by precision and accuracy evaluation, concordance tests, case sample tests, sensitivity, species specificity, stability, stutter calculation, and DNA mixtures, according to the guidelines described by the Scientific Working Group on DNA Analysis Methods (SWGDAM) and regulations published by the China Ministry of Public Security. The validation results indicate the robustness and reliability of this new system, and it could be a potentially helpful tool for human identification and paternity testing in the Chinese population, as well as facilitating global forensic DNA data sharing.

Authors+Show Affiliations

Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China. Department of Forensic Medicine, School of Basic Medical Science, Wenzhou Medical University, Wenzhou, P. R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China. School of Basic Medicine, Inner Mongolia Medical University, Hohhot, P. R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China. Clinical Medical School of Inner Mongolia University for the Nationalities, Tongliao, P. R. China.PeopleSpot Inc., Beijing, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China.Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33864289

Citation

Qu, Yiling, et al. "Development and Validation of a Forensic Six-dye Multiplex Assay With 29 STR Loci." Electrophoresis, vol. 42, no. 14-15, 2021, pp. 1419-1430.
Qu Y, Tao R, Yu H, et al. Development and validation of a forensic six-dye multiplex assay with 29 STR loci. Electrophoresis. 2021;42(14-15):1419-1430.
Qu, Y., Tao, R., Yu, H., Yang, Q., Wang, Z., Tan, R., Zhang, X., Xia, R., Xiong, L., Xi, S., Wu, J., Gao, Y., Zhang, S., & Li, C. (2021). Development and validation of a forensic six-dye multiplex assay with 29 STR loci. Electrophoresis, 42(14-15), 1419-1430. https://doi.org/10.1002/elps.202100019
Qu Y, et al. Development and Validation of a Forensic Six-dye Multiplex Assay With 29 STR Loci. Electrophoresis. 2021;42(14-15):1419-1430. PubMed PMID: 33864289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of a forensic six-dye multiplex assay with 29 STR loci. AU - Qu,Yiling, AU - Tao,RuiYang, AU - Yu,Huan, AU - Yang,Qi, AU - Wang,Ziwei, AU - Tan,Rui, AU - Zhang,Xiaochun, AU - Xia,Ruocheng, AU - Xiong,Lei, AU - Xi,Shihan, AU - Wu,Jun, AU - Gao,Yuzhen, AU - Zhang,Suhua, AU - Li,Chengtao, Y1 - 2021/05/04/ PY - 2021/03/05/revised PY - 2021/01/18/received PY - 2021/04/10/accepted PY - 2021/4/18/pubmed PY - 2021/4/18/medline PY - 2021/4/17/entrez KW - Capillary electrophoresis KW - Developmental validation KW - Forensic genetics KW - Multiplex PCR assay KW - Short tandem repeats SP - 1419 EP - 1430 JF - Electrophoresis JO - Electrophoresis VL - 42 IS - 14-15 N2 - This paper describes the development and validation of a novel 31-locus, six-dye STR multiplex system, which is designed to meet the needs of the rapidly growing Chinese forensic database. This new assay combines 20 extended-CODIS core loci (D3S1358, D5S818, TPOX, CSF1PO, TH01, vWA, D7S820, D21S11, D8S1179, D18S51, D16S539, D13S317, FGA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433, and D22S1045), nine highly polymorphic loci in Chinese Han population (D3S3045, D6S1043, D6S477, D8S1132, D10S1435, D15S659, D19S253, Penta D, and Penta E), and two gender determining markers, amelogenin and Y-Indel, which could amplify DNA from extracts, as well as direct amplification from substrates. To demonstrate the suitability for forensic applications, this system was validated by precision and accuracy evaluation, concordance tests, case sample tests, sensitivity, species specificity, stability, stutter calculation, and DNA mixtures, according to the guidelines described by the Scientific Working Group on DNA Analysis Methods (SWGDAM) and regulations published by the China Ministry of Public Security. The validation results indicate the robustness and reliability of this new system, and it could be a potentially helpful tool for human identification and paternity testing in the Chinese population, as well as facilitating global forensic DNA data sharing. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/33864289/Development_and_validation_of_a_forensic_six_dye_multiplex_assay_with_29_STR_loci_ L2 - https://doi.org/10.1002/elps.202100019 DB - PRIME DP - Unbound Medicine ER -
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