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Effects of Different Continuous Cropping Years on Bacterial Community and Diversity of Cucumber Rhizosphere Soil in Solar-Greenhouse.
Curr Microbiol. 2021 Jun; 78(6):2380-2390.CM

Abstract

The rhizosphere soils from 1, 3, 5, and 7 years of cucumber continuous cropping in solar-greenhouse were used as the research objects. The region of bacterial 16S rRNA was analyzed by Illumina MiSeq high-throughput sequencing technology. The effect of continuous cropping years on the microbial community structure and diversity in cucumber soil in the greenhouse was investigated. The physical and chemical properties of soil and the activities of urease and catalase were determined. The results showed that cucumber crop succession for different years affected the community composition of the bacteria at the phylum level, and the abundance of Proteobacteria, Chloroflexi, Gemmatimonadetes, Patescibacteria and Firmicutes gradually increased, while Actinobacteria in the soil significantly decreased. Among the top 15 significantly different genera, with the extension of successive years, the relative abundance of most genera in bacteria decreased after a small increase in year 3. The diversity results indicated that soil samples from continuous cropping for 7 years had the lowest community diversity. PICRUSt analysis showed a decreasing trend in soil bacterial function as the cucumber crop succession age increased. In environmental factor clustering analysis, the soil bacterial community was significantly correlated with pH, available nitrogen (AN), soil urease (SUR) and available phosphorus (AP), and the effect on the bacterial community was expressed as SUR > AP > AN > pH.

Authors+Show Affiliations

Microbial Research Institute of Liaoning Province, No. 820 Longshan Street, Shuangta District, Chaoyang, 122000, China.Microbial Research Institute of Liaoning Province, No. 820 Longshan Street, Shuangta District, Chaoyang, 122000, China.Microbial Research Institute of Liaoning Province, No. 820 Longshan Street, Shuangta District, Chaoyang, 122000, China.Microbial Research Institute of Liaoning Province, No. 820 Longshan Street, Shuangta District, Chaoyang, 122000, China.Microbial Research Institute of Liaoning Province, No. 820 Longshan Street, Shuangta District, Chaoyang, 122000, China.Microbial Research Institute of Liaoning Province, No. 820 Longshan Street, Shuangta District, Chaoyang, 122000, China.Microbial Research Institute of Liaoning Province, No. 820 Longshan Street, Shuangta District, Chaoyang, 122000, China. zhuweiwei@vip.163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33871692

Citation

Li, Yang, et al. "Effects of Different Continuous Cropping Years On Bacterial Community and Diversity of Cucumber Rhizosphere Soil in Solar-Greenhouse." Current Microbiology, vol. 78, no. 6, 2021, pp. 2380-2390.
Li Y, Chi J, Ao J, et al. Effects of Different Continuous Cropping Years on Bacterial Community and Diversity of Cucumber Rhizosphere Soil in Solar-Greenhouse. Curr Microbiol. 2021;78(6):2380-2390.
Li, Y., Chi, J., Ao, J., Gao, X., Liu, X., Sun, Y., & Zhu, W. (2021). Effects of Different Continuous Cropping Years on Bacterial Community and Diversity of Cucumber Rhizosphere Soil in Solar-Greenhouse. Current Microbiology, 78(6), 2380-2390. https://doi.org/10.1007/s00284-021-02485-x
Li Y, et al. Effects of Different Continuous Cropping Years On Bacterial Community and Diversity of Cucumber Rhizosphere Soil in Solar-Greenhouse. Curr Microbiol. 2021;78(6):2380-2390. PubMed PMID: 33871692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Different Continuous Cropping Years on Bacterial Community and Diversity of Cucumber Rhizosphere Soil in Solar-Greenhouse. AU - Li,Yang, AU - Chi,Jingliang, AU - Ao,Jing, AU - Gao,Xiaomei, AU - Liu,Xiaohui, AU - Sun,Yulu, AU - Zhu,Weiwei, Y1 - 2021/04/19/ PY - 2020/07/01/received PY - 2021/03/29/accepted PY - 2021/4/20/pubmed PY - 2021/6/1/medline PY - 2021/4/19/entrez SP - 2380 EP - 2390 JF - Current microbiology JO - Curr Microbiol VL - 78 IS - 6 N2 - The rhizosphere soils from 1, 3, 5, and 7 years of cucumber continuous cropping in solar-greenhouse were used as the research objects. The region of bacterial 16S rRNA was analyzed by Illumina MiSeq high-throughput sequencing technology. The effect of continuous cropping years on the microbial community structure and diversity in cucumber soil in the greenhouse was investigated. The physical and chemical properties of soil and the activities of urease and catalase were determined. The results showed that cucumber crop succession for different years affected the community composition of the bacteria at the phylum level, and the abundance of Proteobacteria, Chloroflexi, Gemmatimonadetes, Patescibacteria and Firmicutes gradually increased, while Actinobacteria in the soil significantly decreased. Among the top 15 significantly different genera, with the extension of successive years, the relative abundance of most genera in bacteria decreased after a small increase in year 3. The diversity results indicated that soil samples from continuous cropping for 7 years had the lowest community diversity. PICRUSt analysis showed a decreasing trend in soil bacterial function as the cucumber crop succession age increased. In environmental factor clustering analysis, the soil bacterial community was significantly correlated with pH, available nitrogen (AN), soil urease (SUR) and available phosphorus (AP), and the effect on the bacterial community was expressed as SUR > AP > AN > pH. SN - 1432-0991 UR - https://www.unboundmedicine.com/medline/citation/33871692/Effects_of_Different_Continuous_Cropping_Years_on_Bacterial_Community_and_Diversity_of_Cucumber_Rhizosphere_Soil_in_Solar_Greenhouse_ DB - PRIME DP - Unbound Medicine ER -