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Use of the stable isotope, 58Fe, for determining availability of nonheme iron in meals.
Pediatr Res. 1988 May; 23(5):495-9.PR

Abstract

Because of reluctance to use radioisotopes for studies of iron absorption in children, we have explored the feasibility of using the least abundant stable isotope of iron, 58Fe (natural abundance, 0.322 weight %) in a study of nonheme iron absorption. With a balanced cross-over design, each of 16 school-age children was fed a standardized lunch on 3 consecutive days and, 28 days later, an alternate standardized lunch on 3 consecutive days. The lunch included either a beef patty or a beef-soy patty. The mass isotope ratio, 58Fe/57Fe (MIR58/57), was measured in blood by inductively coupled plasma mass spectroscopy before and 14 days after (i.e. study day 15) consuming the three lunches. The MIR58/57 on study day 15 was used as a baseline value for lunches fed on study days 29, 30, and 31. Incorporation of 58Fe into erythrocytes was greater from the lunch with beef patty than from the lunch with beef-soy patty (geometric mean values 2.02 and 1.05% of the dose, p less than 0.03). Based on the similarity of our results with those obtained in adults with radioisotopes, we conclude that 58Fe is a satisfactory tag for studies of nonheme iron absorption from meals.

Authors+Show Affiliations

Woodhead JC
Department of Pediatrics, College of Medicine, University of Iowa, Iowa City 52242.
Drulis JM
No affiliation info available
Rogers RR
No affiliation info available
Ziegler EE
No affiliation info available
Stumbo PJ
No affiliation info available
Janghorbani M
No affiliation info available
Ting BT
No affiliation info available
Fomon SJ
No affiliation info available

MeSH

ChildDietErythrocytesFemaleFood AnalysisHumansIron IsotopesMaleMass SpectrometryMetalloproteinsNonheme Iron Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3387171

Citation

Woodhead, J C., et al. "Use of the Stable Isotope, 58Fe, for Determining Availability of Nonheme Iron in Meals." Pediatric Research, vol. 23, no. 5, 1988, pp. 495-9.
Woodhead JC, Drulis JM, Rogers RR, et al. Use of the stable isotope, 58Fe, for determining availability of nonheme iron in meals. Pediatr Res. 1988;23(5):495-9.
Woodhead, J. C., Drulis, J. M., Rogers, R. R., Ziegler, E. E., Stumbo, P. J., Janghorbani, M., Ting, B. T., & Fomon, S. J. (1988). Use of the stable isotope, 58Fe, for determining availability of nonheme iron in meals. Pediatric Research, 23(5), 495-9.
Woodhead JC, et al. Use of the Stable Isotope, 58Fe, for Determining Availability of Nonheme Iron in Meals. Pediatr Res. 1988;23(5):495-9. PubMed PMID: 3387171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of the stable isotope, 58Fe, for determining availability of nonheme iron in meals. AU - Woodhead,J C, AU - Drulis,J M, AU - Rogers,R R, AU - Ziegler,E E, AU - Stumbo,P J, AU - Janghorbani,M, AU - Ting,B T, AU - Fomon,S J, PY - 1988/5/1/pubmed PY - 1988/5/1/medline PY - 1988/5/1/entrez SP - 495 EP - 9 JF - Pediatric research JO - Pediatr Res VL - 23 IS - 5 N2 - Because of reluctance to use radioisotopes for studies of iron absorption in children, we have explored the feasibility of using the least abundant stable isotope of iron, 58Fe (natural abundance, 0.322 weight %) in a study of nonheme iron absorption. With a balanced cross-over design, each of 16 school-age children was fed a standardized lunch on 3 consecutive days and, 28 days later, an alternate standardized lunch on 3 consecutive days. The lunch included either a beef patty or a beef-soy patty. The mass isotope ratio, 58Fe/57Fe (MIR58/57), was measured in blood by inductively coupled plasma mass spectroscopy before and 14 days after (i.e. study day 15) consuming the three lunches. The MIR58/57 on study day 15 was used as a baseline value for lunches fed on study days 29, 30, and 31. Incorporation of 58Fe into erythrocytes was greater from the lunch with beef patty than from the lunch with beef-soy patty (geometric mean values 2.02 and 1.05% of the dose, p less than 0.03). Based on the similarity of our results with those obtained in adults with radioisotopes, we conclude that 58Fe is a satisfactory tag for studies of nonheme iron absorption from meals. SN - 0031-3998 UR - https://www.unboundmedicine.com/medline/citation/3387171/Use_of_the_stable_isotope_58Fe_for_determining_availability_of_nonheme_iron_in_meals_ DB - PRIME DP - Unbound Medicine ER -