TY - JOUR T1 - Simultaneous quantification of 18 different phytocannabinoids in serum using a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. AU - Scheunemann,A, AU - Elsner,K, AU - Germerott,T, AU - Hess,C, AU - Röhrich,J, Y1 - 2021/04/01/ PY - 2020/08/20/received PY - 2021/02/22/revised PY - 2021/03/29/accepted PY - 2021/4/22/pubmed PY - 2021/4/22/medline PY - 2021/4/21/entrez KW - Cannabinoids KW - LC-MS/MS KW - Method validation KW - Serum level KW - Solid phase extraction SP - 122685 EP - 122685 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 1173 N2 - The potential therapeutic effects of various phytocannabinoids and the availability of multiple cannabis-based medicines make it desirable to have an analytical method that simultaneously quantifies a wide range of cannabinoids in blood, beyond delta-9-tetrahydrocannabinol and its metabolites. A liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of 18 phytocannabinoids and cannabinoid metabolites in serum was developed and validated. The method enables simultaneous detection of delta-9-tetrahydrocannabinol, cannabidiol, cannabinol, cannabigerol, cannabichromene, cannabicyclol, tetrahydrocannabivarin and cannabidivarin and their acidic precursors tetrahydocannabinolic acid A, cannabidiolic acid, cannabinolic acid, cannabigerolic acid, cannabichromenic acid, cannabicyclolic acid, tetrahydrocannabivarinic acid and cannabidivarinic acid as well as the delta-9-tetrahydrocannabinol metabolites 11-hydroxy-tetrahydrocannabinol and 11-nor-9-carboxy-tetrahydrocannabinol. Limits of detection ranged from 0.0004 to 1 ng/mL and limits of quantification ranged from 0.004 to 2 ng/mL. Calibration curves of all analytes were linear over the whole calibration range. Recovery rates of 52 to 86% were obtained for all analytes except for cannabicyclol (49%), 11-nor-9-carboxy-tetrahydrocannabinol (46%), cannabichromenic acid (44%) and cannabidivarinic acid (36%). Acceptable bias and precision data were demonstrated for all analytes. The method was successfully applied to 55 forensic serum samples, obtained from the Institute of Legal Medicine Mainz. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/33882447/Simultaneous_quantification_of_18_different_phytocannabinoids_in_serum_using_a_highly_sensitive_liquid_chromatography_tandem_mass_spectrometry__LC_MS/MS__method_ DB - PRIME DP - Unbound Medicine ER -