Tags

Type your tag names separated by a space and hit enter

Glutathione peroxidase activity, lipid peroxides and selenium concentration in various rat organs.
Biomed Biochim Acta. 1988; 47(1):19-24.BB

Abstract

Total and selenium-dependent glutathione peroxidase activity, lipid peroxides and selenium concentration were determined in tissues of rats kept upon standard diet. The selenium concentration as calculated per mg of protein is as follows: liver greater than kidney greater than lung greater than heart greater than muscles greater than brain greater than erythrocytes. The lipid peroxide concentration as expressed by the malondialdehyde amount is a follows: brain greater than heart greater than muscles greater than lung greater than kidney greater than liver. In all the analyzed tissues, Se-GSH-Px activity was found as measured with t-butyl hydroperoxide as substrate, and total GSH-Px activity, as assayed with cumene hydroperoxide. A highly significant correlation was found between Se-GSH-Px activity and selenium concentration in selected tissues (r = 0.91, p less than 0.01), and an inverse linear correlation between lipid peroxides concentration and Se-GSH-Px activity (r = -0.75, p less than 0.1). Se-independent GSH-Px activity was estimated as the difference between total GSH-Px and Se-GSH-Px activity. In all the investigated rat tissues higher activity of Se-dependent GSH-Px than that of Se-independent GSH-Px was observed. In erythrocytes and muscles only the selenium dependent enzyme was detected. Also glutathione S-transferase activity was estimated in the above tissues. No GSH S-transferase activity was found in rat erythrocytes.

Authors+Show Affiliations

Department of Biochemistry INP WAM, Medical Academy, Lódź, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3390165

Citation

Gromadzińska, J, et al. "Glutathione Peroxidase Activity, Lipid Peroxides and Selenium Concentration in Various Rat Organs." Biomedica Biochimica Acta, vol. 47, no. 1, 1988, pp. 19-24.
Gromadzińska J, Skłodowska M, Wasowicz W. Glutathione peroxidase activity, lipid peroxides and selenium concentration in various rat organs. Biomed Biochim Acta. 1988;47(1):19-24.
Gromadzińska, J., Skłodowska, M., & Wasowicz, W. (1988). Glutathione peroxidase activity, lipid peroxides and selenium concentration in various rat organs. Biomedica Biochimica Acta, 47(1), 19-24.
Gromadzińska J, Skłodowska M, Wasowicz W. Glutathione Peroxidase Activity, Lipid Peroxides and Selenium Concentration in Various Rat Organs. Biomed Biochim Acta. 1988;47(1):19-24. PubMed PMID: 3390165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutathione peroxidase activity, lipid peroxides and selenium concentration in various rat organs. AU - Gromadzińska,J, AU - Skłodowska,M, AU - Wasowicz,W, PY - 1988/1/1/pubmed PY - 1988/1/1/medline PY - 1988/1/1/entrez SP - 19 EP - 24 JF - Biomedica biochimica acta JO - Biomed Biochim Acta VL - 47 IS - 1 N2 - Total and selenium-dependent glutathione peroxidase activity, lipid peroxides and selenium concentration were determined in tissues of rats kept upon standard diet. The selenium concentration as calculated per mg of protein is as follows: liver greater than kidney greater than lung greater than heart greater than muscles greater than brain greater than erythrocytes. The lipid peroxide concentration as expressed by the malondialdehyde amount is a follows: brain greater than heart greater than muscles greater than lung greater than kidney greater than liver. In all the analyzed tissues, Se-GSH-Px activity was found as measured with t-butyl hydroperoxide as substrate, and total GSH-Px activity, as assayed with cumene hydroperoxide. A highly significant correlation was found between Se-GSH-Px activity and selenium concentration in selected tissues (r = 0.91, p less than 0.01), and an inverse linear correlation between lipid peroxides concentration and Se-GSH-Px activity (r = -0.75, p less than 0.1). Se-independent GSH-Px activity was estimated as the difference between total GSH-Px and Se-GSH-Px activity. In all the investigated rat tissues higher activity of Se-dependent GSH-Px than that of Se-independent GSH-Px was observed. In erythrocytes and muscles only the selenium dependent enzyme was detected. Also glutathione S-transferase activity was estimated in the above tissues. No GSH S-transferase activity was found in rat erythrocytes. SN - 0232-766X UR - https://www.unboundmedicine.com/medline/citation/3390165/Glutathione_peroxidase_activity_lipid_peroxides_and_selenium_concentration_in_various_rat_organs_ DB - PRIME DP - Unbound Medicine ER -