TY - JOUR T1 - Expression map of entry receptors and infectivity factors for pan-coronaviruses in preimplantation and implantation stage human embryos. AU - Colaco,S, AU - Chhabria,K, AU - Singh,D, AU - Bhide,A, AU - Singh,N, AU - Singh,A, AU - Husein,A, AU - Mishra,A, AU - Sharma,R, AU - Ashary,N, AU - Modi,D, Y1 - 2021/04/28/ PY - 2020/12/02/received PY - 2021/04/08/accepted PY - 2021/4/30/pubmed PY - 2021/8/10/medline PY - 2021/4/29/entrez KW - ACE2 KW - COVID-19 KW - Coronavirus KW - In vitro fertilization KW - Interaction KW - Replication KW - SARS-CoV-2 KW - TMPRSS2 KW - Virus KW - scRNAseq SP - 1709 EP - 1720 JF - Journal of assisted reproduction and genetics JO - J Assist Reprod Genet VL - 38 IS - 7 N2 - PURPOSE: To predict if developing human embryos are permissive to multiple coronaviruses. METHOD: We analyzed publicly available single-cell RNA-seq datasets of human embryos for the known canonical and non-canonical receptors and spike protein cleavage enzymes for multiple coronaviruses like SARS-CoV, SARS-CoV-2, MERS-CoV, hCoV-229E, and hCoV-NL63. We also analyzed the expression of host genes involved in viral replication, host proteins involved in viral endosomal sorting complexes required for transport (ESCRT), genes of host proteins that physically interact with proteins of SARS-CoV-2, and the host genes essential for coronavirus infectivity. RESULTS: Of the known receptors of SARS viruses, ACE2, BSG, GOLGA7, and ZDHHC5 were expressed in different proportions in the zygote, 4-cell, 8-cell, morula, and blastocysts including the trophectoderm. The MERS-CoV receptor, DPP4, and hCoV-229E receptor, ANPEP, were expressed mainly from the compact morula to the blastocyst stages. Transcripts of the MERS-CoV alternate receptor LGALS1 were detected in most cells at all stages of development. TMPRSS2 transcripts were detected in the epiblast, primitive endoderm, and trophectoderm, while transcripts of the endosomal proteases CTSL, CTSB, and FURIN were expressed in most cells at all stages of development. ACE2 and TMPRSS2 were co-expressed in a proportion of epiblast and trophectoderm cells. The embryonic cells expressed genes involved in ESCRT, viral replication, SARS-CoV-2 interactions, and coronavirus infectivity. The ACE2 and TMPRSS2 co-expressing cells were enriched in genes associated with lipid metabolism, lysosome, peroxisome, and oxidative phosphorylation pathways. CONCLUSION: Preimplantation and implantation stage human embryos could be permissive to multiple hCoVs. SN - 1573-7330 UR - https://www.unboundmedicine.com/medline/citation/33913101/Expression_map_of_entry_receptors_and_infectivity_factors_for_pan_coronaviruses_in_preimplantation_and_implantation_stage_human_embryos_ DB - PRIME DP - Unbound Medicine ER -