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IgE antibodies to D. pteronyssinus in atopic patients.
Immunology. 1988 Jun; 64(2):311-4.I

Abstract

An aqueous house dust mite extract was separated by SDS-PAGE and transferred to nitrocellulose membranes by Western Blotting. Two major allergens, Der pI and Der pII associated with the mite faeces and body, respectively, were identified on the protein blot. The blot was then probed with atopic sera and the bound antibodies were labelled with 125I anti-IgE and visualized by autoradiography. Using this technique it is shown that serum samples from eczema patients contain a higher proportion of anti-mite body IgE antibodies, whereas those from asthma patients contain a higher proportion of antibodies against the major mite faecal allergen Der pI. Minor mite body allergens are also shown to be important in eczematous patients. The role of mite allergens in atopic conditions is discussed, along with the significance of the findings presented here.

Authors+Show Affiliations

Department of Child Health, University of Bristol, U.K.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3391646

Citation

Thompson, S J., et al. "IgE Antibodies to D. Pteronyssinus in Atopic Patients." Immunology, vol. 64, no. 2, 1988, pp. 311-4.
Thompson SJ, Whitley HJ, Naysmith JD, et al. IgE antibodies to D. pteronyssinus in atopic patients. Immunology. 1988;64(2):311-4.
Thompson, S. J., Whitley, H. J., Naysmith, J. D., & Carswell, F. (1988). IgE antibodies to D. pteronyssinus in atopic patients. Immunology, 64(2), 311-4.
Thompson SJ, et al. IgE Antibodies to D. Pteronyssinus in Atopic Patients. Immunology. 1988;64(2):311-4. PubMed PMID: 3391646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IgE antibodies to D. pteronyssinus in atopic patients. AU - Thompson,S J, AU - Whitley,H J, AU - Naysmith,J D, AU - Carswell,F, PY - 1988/6/1/pubmed PY - 1988/6/1/medline PY - 1988/6/1/entrez SP - 311 EP - 4 JF - Immunology JO - Immunology VL - 64 IS - 2 N2 - An aqueous house dust mite extract was separated by SDS-PAGE and transferred to nitrocellulose membranes by Western Blotting. Two major allergens, Der pI and Der pII associated with the mite faeces and body, respectively, were identified on the protein blot. The blot was then probed with atopic sera and the bound antibodies were labelled with 125I anti-IgE and visualized by autoradiography. Using this technique it is shown that serum samples from eczema patients contain a higher proportion of anti-mite body IgE antibodies, whereas those from asthma patients contain a higher proportion of antibodies against the major mite faecal allergen Der pI. Minor mite body allergens are also shown to be important in eczematous patients. The role of mite allergens in atopic conditions is discussed, along with the significance of the findings presented here. SN - 0019-2805 UR - https://www.unboundmedicine.com/medline/citation/3391646/IgE_antibodies_to_D__pteronyssinus_in_atopic_patients_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/3391646/ DB - PRIME DP - Unbound Medicine ER -