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MIR172d Is Required for Floral Organ Identity and Number in Tomato.
Int J Mol Sci. 2021 Apr 28; 22(9)IJ

Abstract

MicroRNA172 (miR172) functions as a central regulator of flowering time and flower development by post-transcriptional repression of APETALA2-LIKE transcription factors. In the model crop Solanum lycopersicum (tomato), the miR172 family is still poorly annotated and information about the functions of specific members is lacking. Here, de-novo prediction of tomato miR172 coding loci identified seven genes (SlMIR172a-g), that code for four unique species of miR172 (sly-miR172). During reproductive development, sly-miR172s are differentially expressed, with sly-miR172c and sly-miR172d being the most abundant members in developing flowers, and are predicted to guide the cleavage of eight APETALA2-LIKE transcription factors. By CRISPR-Cas9 co-targeting of SlMIR172c and SlMIR172d we have generated a battery of loss-of-function and hypomorphic mutants (slmir172c-dCR). The slmir172c-dCR plants developed normal shoot but their flowers displayed graded floral organ abnormalities. Whereas slmir172cCR loss-of-function caused only a slight greening of petals and stamens, hypomorphic and loss-of-function slmir172dCR alleles were associated with the conversion of petals and stamens to sepaloids, which were produced in excess. Interestingly, the degrees of floral organ identity alteration and proliferation were directly correlated with the reduction in sly-miR172d activity. These results suggest that sly-miR172d regulates in a dose-dependent manner floral organ identity and number, likely by negatively regulating its APETALA2-like targets.

Authors+Show Affiliations

Institute of Plant Sciences, Agricultural Research Organization-Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel. Department of Plant Science, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, Israel.Institute of Plant Sciences, Agricultural Research Organization-Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel.Institute of Plant Sciences, Agricultural Research Organization-Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel.Institute of Plant Sciences, Agricultural Research Organization-Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33925088

Citation

Lin, Wanping, et al. "MIR172d Is Required for Floral Organ Identity and Number in Tomato." International Journal of Molecular Sciences, vol. 22, no. 9, 2021.
Lin W, Gupta SK, Arazi T, et al. MIR172d Is Required for Floral Organ Identity and Number in Tomato. Int J Mol Sci. 2021;22(9).
Lin, W., Gupta, S. K., Arazi, T., & Spitzer-Rimon, B. (2021). MIR172d Is Required for Floral Organ Identity and Number in Tomato. International Journal of Molecular Sciences, 22(9). https://doi.org/10.3390/ijms22094659
Lin W, et al. MIR172d Is Required for Floral Organ Identity and Number in Tomato. Int J Mol Sci. 2021 Apr 28;22(9) PubMed PMID: 33925088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MIR172d Is Required for Floral Organ Identity and Number in Tomato. AU - Lin,Wanping, AU - Gupta,Suresh Kumar, AU - Arazi,Tzahi, AU - Spitzer-Rimon,Ben, Y1 - 2021/04/28/ PY - 2021/03/25/received PY - 2021/04/21/revised PY - 2021/04/26/accepted PY - 2021/4/30/entrez PY - 2021/5/1/pubmed PY - 2021/5/25/medline KW - APETALA KW - CRISPR KW - Cas9 KW - flower KW - miR172 KW - miRNA KW - tomato JF - International journal of molecular sciences JO - Int J Mol Sci VL - 22 IS - 9 N2 - MicroRNA172 (miR172) functions as a central regulator of flowering time and flower development by post-transcriptional repression of APETALA2-LIKE transcription factors. In the model crop Solanum lycopersicum (tomato), the miR172 family is still poorly annotated and information about the functions of specific members is lacking. Here, de-novo prediction of tomato miR172 coding loci identified seven genes (SlMIR172a-g), that code for four unique species of miR172 (sly-miR172). During reproductive development, sly-miR172s are differentially expressed, with sly-miR172c and sly-miR172d being the most abundant members in developing flowers, and are predicted to guide the cleavage of eight APETALA2-LIKE transcription factors. By CRISPR-Cas9 co-targeting of SlMIR172c and SlMIR172d we have generated a battery of loss-of-function and hypomorphic mutants (slmir172c-dCR). The slmir172c-dCR plants developed normal shoot but their flowers displayed graded floral organ abnormalities. Whereas slmir172cCR loss-of-function caused only a slight greening of petals and stamens, hypomorphic and loss-of-function slmir172dCR alleles were associated with the conversion of petals and stamens to sepaloids, which were produced in excess. Interestingly, the degrees of floral organ identity alteration and proliferation were directly correlated with the reduction in sly-miR172d activity. These results suggest that sly-miR172d regulates in a dose-dependent manner floral organ identity and number, likely by negatively regulating its APETALA2-like targets. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/33925088/MIR172d_Is_Required_for_Floral_Organ_Identity_and_Number_in_Tomato_ L2 - https://www.mdpi.com/resolver?pii=ijms22094659 DB - PRIME DP - Unbound Medicine ER -