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Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019.
BMC Infect Dis. 2021 May 01; 21(1):405.BI

Abstract

BACKGROUND

Aseptic meningitis is most often caused by enteroviruses (EVs), but EVs associated with aseptic meningitis have not yet been reported in Liaocheng. The aim of this study was to determine the prevalence and genetic characteristics of EVs causing aseptic meningitis in children in Liaocheng.

METHODS

We reviewed the epidemiological and clinical characteristics of 504 paediatric cases of aseptic meningitis in Liaocheng from 2018 to 2019 and analysed the phylogeny of the predominant EV types causing this disease.

RESULTS

A total of 107 children were positive for EV in cerebrospinal fluid samples by nested PCR. Most of the positive patients were children 13 years old or younger and had symptoms such as fever, headache and vomiting (P < 0.05). The seasons with the highest prevalence of EV-positive cases were summer and autumn. The 107 EV sequences belonged to 8 serotypes, and echovirus types 18, 6 and 11 were the three dominant serotypes in Liaocheng during the 2-year study period. Phylogenetic analyses demonstrated that the E18 and E6 isolates belonged to subgenotype C2, while the E11 isolates belonged to subgenotype D5. VP1 analysis suggested that only one lineage of these three types was cocirculating in the Liaocheng region.

CONCLUSIONS

This study demonstrated the diverse EV genotypes contributing to a large outbreak of aseptic meningitis in Liaocheng. Therefore, large-scale surveillance is required to assess the epidemiology of EVs associated with aseptic meningitis and is important for the diagnosis and treatment of aseptic meningitis in Liaocheng.

Authors+Show Affiliations

Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China.Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China.Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China.Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China. tinawang1003@163.com.Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China.Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China.Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China.Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China.Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33933008

Citation

Wang, Jing, et al. "Analysis of Enterovirus Genotypes in the Cerebrospinal Fluid of Children Associated With Aseptic Meningitis in Liaocheng, China, From 2018 to 2019." BMC Infectious Diseases, vol. 21, no. 1, 2021, p. 405.
Wang J, Meng M, Xu H, et al. Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019. BMC Infect Dis. 2021;21(1):405.
Wang, J., Meng, M., Xu, H., Wang, T., Liu, Y., Yan, H., Liu, P., Qin, D., & Yang, Q. (2021). Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019. BMC Infectious Diseases, 21(1), 405. https://doi.org/10.1186/s12879-021-06112-9
Wang J, et al. Analysis of Enterovirus Genotypes in the Cerebrospinal Fluid of Children Associated With Aseptic Meningitis in Liaocheng, China, From 2018 to 2019. BMC Infect Dis. 2021 May 1;21(1):405. PubMed PMID: 33933008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019. AU - Wang,Jing, AU - Meng,Min, AU - Xu,Huan, AU - Wang,Ting, AU - Liu,Ying, AU - Yan,Han, AU - Liu,Peiman, AU - Qin,Daogang, AU - Yang,Qiaozhi, Y1 - 2021/05/01/ PY - 2020/12/21/received PY - 2021/04/23/accepted PY - 2021/5/2/entrez PY - 2021/5/3/pubmed PY - 2021/5/12/medline KW - Aseptic meningitis KW - Children KW - Enterovirus (EV) KW - Liaocheng KW - Phylogenetic analysis SP - 405 EP - 405 JF - BMC infectious diseases JO - BMC Infect Dis VL - 21 IS - 1 N2 - BACKGROUND: Aseptic meningitis is most often caused by enteroviruses (EVs), but EVs associated with aseptic meningitis have not yet been reported in Liaocheng. The aim of this study was to determine the prevalence and genetic characteristics of EVs causing aseptic meningitis in children in Liaocheng. METHODS: We reviewed the epidemiological and clinical characteristics of 504 paediatric cases of aseptic meningitis in Liaocheng from 2018 to 2019 and analysed the phylogeny of the predominant EV types causing this disease. RESULTS: A total of 107 children were positive for EV in cerebrospinal fluid samples by nested PCR. Most of the positive patients were children 13 years old or younger and had symptoms such as fever, headache and vomiting (P < 0.05). The seasons with the highest prevalence of EV-positive cases were summer and autumn. The 107 EV sequences belonged to 8 serotypes, and echovirus types 18, 6 and 11 were the three dominant serotypes in Liaocheng during the 2-year study period. Phylogenetic analyses demonstrated that the E18 and E6 isolates belonged to subgenotype C2, while the E11 isolates belonged to subgenotype D5. VP1 analysis suggested that only one lineage of these three types was cocirculating in the Liaocheng region. CONCLUSIONS: This study demonstrated the diverse EV genotypes contributing to a large outbreak of aseptic meningitis in Liaocheng. Therefore, large-scale surveillance is required to assess the epidemiology of EVs associated with aseptic meningitis and is important for the diagnosis and treatment of aseptic meningitis in Liaocheng. SN - 1471-2334 UR - https://www.unboundmedicine.com/medline/citation/33933008/Analysis_of_enterovirus_genotypes_in_the_cerebrospinal_fluid_of_children_associated_with_aseptic_meningitis_in_Liaocheng_China_from_2018_to_2019_ L2 - https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-021-06112-9 DB - PRIME DP - Unbound Medicine ER -