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Stigmasterol isolated from the chloroform fraction of Adenopus breviflorus Benth fruit induces mitochondrial-dependent apoptosis in rat liver.
J Complement Integr Med. 2021 May 10; 18(4):737-744.JC

Abstract

OBJECTIVES

Decoction of Adenopus breviflorus fruit is used in folkloric medicine for treating dysmenorrhea and gonorrhea. Phytochemicals from A. breviflorus may be potent in inducing mitochondrial-dependent apoptosis via the opening of the mitochondrial permeability transition (MPT) pore. Therefore, this study investigated the in vitro effects of stigmasterol isolated from the chloroform fraction of A. breviflorus (CFAB) and also the increasing concentration of CFAB on the opening of rat liver mitochondrial permeability transition (MPT) pore.

METHODS

Fractionation of CFAB on column chromatography yielded a needle-like crystal which structure was elucidated by standard spectroscopic techniques. The effects of stigmasterol and CFAB on MPT pore opening were assayed spectrophotometrically. Also, the effect of CFAB on mitochondrial ATPase (mATPase) activity and cytochrome c (Cyt c) release were determined.

RESULTS

Stigmasterol isolated from CFAB induced MPT pore opening significantly (p<0.05) when compared with the control. Similarly, CFAB significantly (p<0.05) induced MPT pore opening in rat liver mitochondria in a concentration-dependent manner in the presence and absence of the triggering agent - calcium ion. Furthermore, the increasing concentration of CFAB significantly (p<0.05) stimulated mitochondrial ATPase (mATPase) activity and Cyt c release in a concentration-dependent manner.

CONCLUSIONS

The study showed that stigmasterol isolated from the chloroform fraction of A. breviflorus is a potent inducer of mitochondrial-dependent apoptosis. Also, the study further revealed that CFAB possesses potent bioactive compounds which can induce the mitochondrial-dependent apoptosis through the opening of the mitochondrial permeability transition pore, activation of mitochondrial ATPase (mATPase) activity and cytochrome c release.

Authors+Show Affiliations

Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry, University of Ibadan, Ibadan, Nigeria.Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry, University of Ibadan, Ibadan, Nigeria.Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry, University of Ibadan, Ibadan, Nigeria.Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry, University of Ibadan, Ibadan, Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33964200

Citation

Oyedeji, Tolulope A., et al. "Stigmasterol Isolated From the Chloroform Fraction of Adenopus Breviflorus Benth Fruit Induces Mitochondrial-dependent Apoptosis in Rat Liver." Journal of Complementary & Integrative Medicine, vol. 18, no. 4, 2021, pp. 737-744.
Oyedeji TA, Onireti DO, Lasisi OS, et al. Stigmasterol isolated from the chloroform fraction of Adenopus breviflorus Benth fruit induces mitochondrial-dependent apoptosis in rat liver. J Complement Integr Med. 2021;18(4):737-744.
Oyedeji, T. A., Onireti, D. O., Lasisi, O. S., Akobi, C. I., & Olorunsogo, O. O. (2021). Stigmasterol isolated from the chloroform fraction of Adenopus breviflorus Benth fruit induces mitochondrial-dependent apoptosis in rat liver. Journal of Complementary & Integrative Medicine, 18(4), 737-744. https://doi.org/10.1515/jcim-2020-0323
Oyedeji TA, et al. Stigmasterol Isolated From the Chloroform Fraction of Adenopus Breviflorus Benth Fruit Induces Mitochondrial-dependent Apoptosis in Rat Liver. J Complement Integr Med. 2021 May 10;18(4):737-744. PubMed PMID: 33964200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stigmasterol isolated from the chloroform fraction of Adenopus breviflorus Benth fruit induces mitochondrial-dependent apoptosis in rat liver. AU - Oyedeji,Tolulope A, AU - Onireti,Daniel O, AU - Lasisi,Olaitan S, AU - Akobi,Chibuzor I, AU - Olorunsogo,Olufunso O, Y1 - 2021/05/10/ PY - 2020/08/12/received PY - 2020/11/12/accepted PY - 2021/5/9/pubmed PY - 2021/12/29/medline PY - 2021/5/8/entrez KW - Adenopus breviflorus KW - apoptosis KW - cytochrome c release KW - mitochondrial permeability transition pore KW - stigmasterol SP - 737 EP - 744 JF - Journal of complementary & integrative medicine JO - J Complement Integr Med VL - 18 IS - 4 N2 - OBJECTIVES: Decoction of Adenopus breviflorus fruit is used in folkloric medicine for treating dysmenorrhea and gonorrhea. Phytochemicals from A. breviflorus may be potent in inducing mitochondrial-dependent apoptosis via the opening of the mitochondrial permeability transition (MPT) pore. Therefore, this study investigated the in vitro effects of stigmasterol isolated from the chloroform fraction of A. breviflorus (CFAB) and also the increasing concentration of CFAB on the opening of rat liver mitochondrial permeability transition (MPT) pore. METHODS: Fractionation of CFAB on column chromatography yielded a needle-like crystal which structure was elucidated by standard spectroscopic techniques. The effects of stigmasterol and CFAB on MPT pore opening were assayed spectrophotometrically. Also, the effect of CFAB on mitochondrial ATPase (mATPase) activity and cytochrome c (Cyt c) release were determined. RESULTS: Stigmasterol isolated from CFAB induced MPT pore opening significantly (p<0.05) when compared with the control. Similarly, CFAB significantly (p<0.05) induced MPT pore opening in rat liver mitochondria in a concentration-dependent manner in the presence and absence of the triggering agent - calcium ion. Furthermore, the increasing concentration of CFAB significantly (p<0.05) stimulated mitochondrial ATPase (mATPase) activity and Cyt c release in a concentration-dependent manner. CONCLUSIONS: The study showed that stigmasterol isolated from the chloroform fraction of A. breviflorus is a potent inducer of mitochondrial-dependent apoptosis. Also, the study further revealed that CFAB possesses potent bioactive compounds which can induce the mitochondrial-dependent apoptosis through the opening of the mitochondrial permeability transition pore, activation of mitochondrial ATPase (mATPase) activity and cytochrome c release. SN - 1553-3840 UR - https://www.unboundmedicine.com/medline/citation/33964200/Stigmasterol_isolated_from_the_chloroform_fraction_of_Adenopus_breviflorus_Benth_fruit_induces_mitochondrial_dependent_apoptosis_in_rat_liver_ L2 - https://www.degruyter.com/document/doi/10.1515/jcim-2020-0323 DB - PRIME DP - Unbound Medicine ER -