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Expression Profiles of Digestive Genes in the Gut and Salivary Glands of Tarnished Plant Bug (Hemiptera: Miridae).
J Insect Sci. 2021 May 01; 21(3)JI

Abstract

Host plant preference of agricultural pests may shift throughout the growing season, allowing the pests to persist on wild hosts when crops are not available. Lygus Hahn (Hemiptera: Miridae) bugs are severe pests of cotton during flowering and fruiting stages, but can persist on alternative crops, or on weed species. Diversity of digestive enzymes produced by salivary glands and gut tissues play a pivotal role in an organism's ability to utilize various food sources. Polyphagous insects produce an array of enzymes that can process carbohydrates, lipids, and proteins. In this study, the digestive enzyme repertoire of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), was identified by high-throughput sequencing followed by cDNA cloning and sequencing. This study identified 87 digestive genes, including 30 polygalacturonases (PG), one β-galactosidase, three α-glucosidases, six β-glucosidases, 28 trypsin-like proteases, three serine proteases, one apyrase-like protease, one cysteine protease, 12 lipases, and two transcripts with low similarity to a xylanase A-like genes. RNA-Seq expression profiles of these digestive genes in adult tarnished plant bugs revealed that 57 and 12 genes were differentially expressed in the salivary gland and gut (≥5-fold, P ≤ 0.01), respectively. All polygalacturonase genes, most proteases, and two xylanase-like genes were differentially expressed in salivary glands, while most of the carbohydrate and lipid processing enzymes were differentially expressed in the gut. Seven of the proteases (KF208689, KF208697, KF208698, KF208699, KF208700, KF208701, and KF208702) were not detected in either the gut or salivary glands.

Authors+Show Affiliations

Southern Insect Management Research Unit, USDA, Agricultural Research Service, Stoneville, MS 38776.Biological Control of Insects Research Laboratory, USDA, Agricultural Research Service, 1503 S. Providence Road, Columbia, MO 65203.Southern Insect Management Research Unit, USDA, Agricultural Research Service, Stoneville, MS 38776.Southern Insect Management Research Unit, USDA, Agricultural Research Service, Stoneville, MS 38776.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33974083

Citation

Perera, Omaththage P., et al. "Expression Profiles of Digestive Genes in the Gut and Salivary Glands of Tarnished Plant Bug (Hemiptera: Miridae)." Journal of Insect Science (Online), vol. 21, no. 3, 2021.
Perera OP, Shelby KS, Pierce CA, et al. Expression Profiles of Digestive Genes in the Gut and Salivary Glands of Tarnished Plant Bug (Hemiptera: Miridae). J Insect Sci. 2021;21(3).
Perera, O. P., Shelby, K. S., Pierce, C. A., & Snodgrass, G. L. (2021). Expression Profiles of Digestive Genes in the Gut and Salivary Glands of Tarnished Plant Bug (Hemiptera: Miridae). Journal of Insect Science (Online), 21(3). https://doi.org/10.1093/jisesa/ieab028
Perera OP, et al. Expression Profiles of Digestive Genes in the Gut and Salivary Glands of Tarnished Plant Bug (Hemiptera: Miridae). J Insect Sci. 2021 May 1;21(3) PubMed PMID: 33974083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression Profiles of Digestive Genes in the Gut and Salivary Glands of Tarnished Plant Bug (Hemiptera: Miridae). AU - Perera,Omaththage P, AU - Shelby,Kent S, AU - Pierce,Calvin A, AU - Snodgrass,Gordon L, PY - 2020/06/16/received PY - 2021/5/11/entrez PY - 2021/5/12/pubmed PY - 2021/5/22/medline KW - RNA-Seq KW - digestive enzyme KW - expression profile KW - salivary gland KW - tarnished plant bug JF - Journal of insect science (Online) JO - J Insect Sci VL - 21 IS - 3 N2 - Host plant preference of agricultural pests may shift throughout the growing season, allowing the pests to persist on wild hosts when crops are not available. Lygus Hahn (Hemiptera: Miridae) bugs are severe pests of cotton during flowering and fruiting stages, but can persist on alternative crops, or on weed species. Diversity of digestive enzymes produced by salivary glands and gut tissues play a pivotal role in an organism's ability to utilize various food sources. Polyphagous insects produce an array of enzymes that can process carbohydrates, lipids, and proteins. In this study, the digestive enzyme repertoire of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), was identified by high-throughput sequencing followed by cDNA cloning and sequencing. This study identified 87 digestive genes, including 30 polygalacturonases (PG), one β-galactosidase, three α-glucosidases, six β-glucosidases, 28 trypsin-like proteases, three serine proteases, one apyrase-like protease, one cysteine protease, 12 lipases, and two transcripts with low similarity to a xylanase A-like genes. RNA-Seq expression profiles of these digestive genes in adult tarnished plant bugs revealed that 57 and 12 genes were differentially expressed in the salivary gland and gut (≥5-fold, P ≤ 0.01), respectively. All polygalacturonase genes, most proteases, and two xylanase-like genes were differentially expressed in salivary glands, while most of the carbohydrate and lipid processing enzymes were differentially expressed in the gut. Seven of the proteases (KF208689, KF208697, KF208698, KF208699, KF208700, KF208701, and KF208702) were not detected in either the gut or salivary glands. SN - 1536-2442 UR - https://www.unboundmedicine.com/medline/citation/33974083/Expression_Profiles_of_Digestive_Genes_in_the_Gut_and_Salivary_Glands_of_Tarnished_Plant_Bug__Hemiptera:_Miridae__ L2 - https://academic.oup.com/jinsectscience/article-lookup/doi/10.1093/jisesa/ieab028 DB - PRIME DP - Unbound Medicine ER -