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Fractions of Ageratum conyzoides L. (Compositae) induce mitochondrial-mediated apoptosis in rats: Possible option in monosodium glutamate-induced hepatic and uterine pathological disorder.
J Ethnopharmacol. 2021 Sep 15; 277:114192.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Several pathological disorders have been attributed to either oxidative stress or defect in apoptotic signaling pathway. Some bioactive compounds elicit their antiproliferative properties by induction of apoptosis via mitochondrial permeability transition (mPT) pore opening.

AIM OF STUDY

The present study therefore investigated the effects of various fractions of methanol extract of Ageratum conyzoides L. (MEAC) on mitochondrial-mediated apoptosis and the possible protective potential of the most potent against monosodium glutamate (MSG)-induced hepatic damage and uterine pathological disorder. The plant is folklorically used in the treatment of cancer and gynecological disorder.

MATERIALS AND METHODS

The MEAC was partitioned in succession and concentrated at 40 °C to obtain chloroform(CFAC), ethylacetate(EFAC) and methanol(MFAC) fractions. Mitochondria were isolated by differential centrifugation. The opening of mPT pore, mATPase activity and hepatic DNA fragmentation were assessed spectrophotometrically. Caspases 9 and 3, SOD and GSH-Px activities and MDA level were determined using ELISA technique. Histological assessment of the liver and uterine sections and GC-MS analysis of the most potent fraction were carried out.

RESULTS

The investigation showed that oral administration of the fractions caused induction of mPT pore opening, enhanced mATPase activity, upregulated the activities of caspases 9 and 3 and also, caused hepatic DNA fragmentation with CFAC being the most potent. The CFAC reversed severe MSG-induced hepatic damage and uterine hyperplasia. The MSG-induced oxidative stress was normalized by CFAC. The GC-MS analysis of CFAC revealed the presence of some pharmacologically relevant phytochemicals.

CONCLUSION

These findings therefore suggest that fractions of Ageratum conyzoides induce mitochondrial-mediated apoptosis. Moreover, CFAC, which is the most potent has a promising antioxidant and antiproliferative potential against MSG-induced hepatic and uterine pathological disorder.

Authors+Show Affiliations

Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria. Electronic address: mr_adeola@yahoo.com.Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria. Electronic address: funsoolorunsogo@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33974943

Citation

Olowofolahan, Adeola Oluwakemi, and Olufunso Olabode Olorunsogo. "Fractions of Ageratum Conyzoides L. (Compositae) Induce Mitochondrial-mediated Apoptosis in Rats: Possible Option in Monosodium Glutamate-induced Hepatic and Uterine Pathological Disorder." Journal of Ethnopharmacology, vol. 277, 2021, p. 114192.
Olowofolahan AO, Olorunsogo OO. Fractions of Ageratum conyzoides L. (Compositae) induce mitochondrial-mediated apoptosis in rats: Possible option in monosodium glutamate-induced hepatic and uterine pathological disorder. J Ethnopharmacol. 2021;277:114192.
Olowofolahan, A. O., & Olorunsogo, O. O. (2021). Fractions of Ageratum conyzoides L. (Compositae) induce mitochondrial-mediated apoptosis in rats: Possible option in monosodium glutamate-induced hepatic and uterine pathological disorder. Journal of Ethnopharmacology, 277, 114192. https://doi.org/10.1016/j.jep.2021.114192
Olowofolahan AO, Olorunsogo OO. Fractions of Ageratum Conyzoides L. (Compositae) Induce Mitochondrial-mediated Apoptosis in Rats: Possible Option in Monosodium Glutamate-induced Hepatic and Uterine Pathological Disorder. J Ethnopharmacol. 2021 Sep 15;277:114192. PubMed PMID: 33974943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fractions of Ageratum conyzoides L. (Compositae) induce mitochondrial-mediated apoptosis in rats: Possible option in monosodium glutamate-induced hepatic and uterine pathological disorder. AU - Olowofolahan,Adeola Oluwakemi, AU - Olorunsogo,Olufunso Olabode, Y1 - 2021/05/08/ PY - 2021/03/23/received PY - 2021/04/28/revised PY - 2021/05/04/accepted PY - 2021/5/12/pubmed PY - 2021/11/24/medline PY - 2021/5/11/entrez KW - Ageratum conyzoides KW - Apoptosis KW - Cancer KW - Mitochondrial permeability transition pore KW - Monosodium glutamate SP - 114192 EP - 114192 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 277 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Several pathological disorders have been attributed to either oxidative stress or defect in apoptotic signaling pathway. Some bioactive compounds elicit their antiproliferative properties by induction of apoptosis via mitochondrial permeability transition (mPT) pore opening. AIM OF STUDY: The present study therefore investigated the effects of various fractions of methanol extract of Ageratum conyzoides L. (MEAC) on mitochondrial-mediated apoptosis and the possible protective potential of the most potent against monosodium glutamate (MSG)-induced hepatic damage and uterine pathological disorder. The plant is folklorically used in the treatment of cancer and gynecological disorder. MATERIALS AND METHODS: The MEAC was partitioned in succession and concentrated at 40 °C to obtain chloroform(CFAC), ethylacetate(EFAC) and methanol(MFAC) fractions. Mitochondria were isolated by differential centrifugation. The opening of mPT pore, mATPase activity and hepatic DNA fragmentation were assessed spectrophotometrically. Caspases 9 and 3, SOD and GSH-Px activities and MDA level were determined using ELISA technique. Histological assessment of the liver and uterine sections and GC-MS analysis of the most potent fraction were carried out. RESULTS: The investigation showed that oral administration of the fractions caused induction of mPT pore opening, enhanced mATPase activity, upregulated the activities of caspases 9 and 3 and also, caused hepatic DNA fragmentation with CFAC being the most potent. The CFAC reversed severe MSG-induced hepatic damage and uterine hyperplasia. The MSG-induced oxidative stress was normalized by CFAC. The GC-MS analysis of CFAC revealed the presence of some pharmacologically relevant phytochemicals. CONCLUSION: These findings therefore suggest that fractions of Ageratum conyzoides induce mitochondrial-mediated apoptosis. Moreover, CFAC, which is the most potent has a promising antioxidant and antiproliferative potential against MSG-induced hepatic and uterine pathological disorder. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/33974943/Fractions_of_Ageratum_conyzoides_L___Compositae__induce_mitochondrial_mediated_apoptosis_in_rats:_Possible_option_in_monosodium_glutamate_induced_hepatic_and_uterine_pathological_disorder_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(21)00419-0 DB - PRIME DP - Unbound Medicine ER -