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Antigliadin antibodies detected by enzyme-linked immunosorbent assay as a marker of childhood celiac disease.
J Pediatr 1988; 113(2):286-9JPed

Abstract

Using an enzyme-linked immunosorbent assay (ELISA) assay, we studied the sera of 17 patients with celiac disease and 114 control subjects for the levels of IgG and IgA antigliadin antibodies. As a group, the patients with celiac disease had significantly higher levels of antigliadin antibodies of both IgG and IgA classes (p less than or equal to 0.001). However, there was a significant overlap of values, resulting in respective sensitivities and specificities of 88% and 90% for IgG antigliadin antibodies, and 73% and 65% for IgA antigliadin antibodies. The combined use of both IgG and IgA antigliadin antibody levels produced a sensitivity of 86% and a specificity of 90%. A gluten-free diet in celiac patients did not seem to affect these results. We conclude that the antigliadin antibody ELISA assay cannot be used as a definitive diagnostic test for celiac disease. The small-bowel biopsy remains the principal diagnostic method; the ELISA assay should, at best, be considered a screening test for this disease.

Authors+Show Affiliations

Department of Pediatrics, University of Texas Health Science Center, San Antonio 78284.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3397791

Citation

Tucker, N T., et al. "Antigliadin Antibodies Detected By Enzyme-linked Immunosorbent Assay as a Marker of Childhood Celiac Disease." The Journal of Pediatrics, vol. 113, no. 2, 1988, pp. 286-9.
Tucker NT, Barghuthy FS, Prihoda TJ, et al. Antigliadin antibodies detected by enzyme-linked immunosorbent assay as a marker of childhood celiac disease. J Pediatr. 1988;113(2):286-9.
Tucker, N. T., Barghuthy, F. S., Prihoda, T. J., Kumar, V., Lerner, A., & Lebenthal, E. (1988). Antigliadin antibodies detected by enzyme-linked immunosorbent assay as a marker of childhood celiac disease. The Journal of Pediatrics, 113(2), pp. 286-9.
Tucker NT, et al. Antigliadin Antibodies Detected By Enzyme-linked Immunosorbent Assay as a Marker of Childhood Celiac Disease. J Pediatr. 1988;113(2):286-9. PubMed PMID: 3397791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigliadin antibodies detected by enzyme-linked immunosorbent assay as a marker of childhood celiac disease. AU - Tucker,N T, AU - Barghuthy,F S, AU - Prihoda,T J, AU - Kumar,V, AU - Lerner,A, AU - Lebenthal,E, PY - 1988/8/1/pubmed PY - 1988/8/1/medline PY - 1988/8/1/entrez SP - 286 EP - 9 JF - The Journal of pediatrics JO - J. Pediatr. VL - 113 IS - 2 N2 - Using an enzyme-linked immunosorbent assay (ELISA) assay, we studied the sera of 17 patients with celiac disease and 114 control subjects for the levels of IgG and IgA antigliadin antibodies. As a group, the patients with celiac disease had significantly higher levels of antigliadin antibodies of both IgG and IgA classes (p less than or equal to 0.001). However, there was a significant overlap of values, resulting in respective sensitivities and specificities of 88% and 90% for IgG antigliadin antibodies, and 73% and 65% for IgA antigliadin antibodies. The combined use of both IgG and IgA antigliadin antibody levels produced a sensitivity of 86% and a specificity of 90%. A gluten-free diet in celiac patients did not seem to affect these results. We conclude that the antigliadin antibody ELISA assay cannot be used as a definitive diagnostic test for celiac disease. The small-bowel biopsy remains the principal diagnostic method; the ELISA assay should, at best, be considered a screening test for this disease. SN - 0022-3476 UR - https://www.unboundmedicine.com/medline/citation/3397791/Antigliadin_antibodies_detected_by_enzyme_linked_immunosorbent_assay_as_a_marker_of_childhood_celiac_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-3476(88)80266-X DB - PRIME DP - Unbound Medicine ER -