Tags

Type your tag names separated by a space and hit enter

Protective effects of citicoline-containing eye drops against UVB-Induced corneal oxidative damage in a rat model.
Exp Eye Res. 2021 07; 208:108612.EE

Abstract

It has been reported that citicoline increases antioxidant activity in some tissues. However, the effect of citicoline on corneal wound-healing has not yet been demonstrated. The aim was to investigate the protective effects of citicoline on ultraviolet B (UVB) radiation-induced corneal oxidative damage in a rat model. Four groups (eight animals each) were investigated: controls; UVB only; UVB/citicoline; and citicoline only. Corneal oxidative damage was induced by exposure to UVB radiation at 560 μW/cm2 for five days in the UVB-exposed groups and 1% citicoline eye drops were applied (3xday) for eight days in the two citicoline groups. Corneal surface damage was evaluated by opacity and fluorescein staining. Corneal injury was assessed biochemically by measuring the concentrations of glutathione (GSH) and malondialdehyde (MDA) and the activity of corneal superoxide dismutase (SOD) and catalase. Matrix metalloproteinase (MMP) -2 and -9 and caspase-3 were evaluated by immunofluorescent staining and microscopic examination and by Western blot analysis. Corneal gene expression analysis was performed for vascular endothelial growth factor (VEGF), interleukin-1 beta (IL-1β) and transforming growth factor-beta (TGF-β). UVB radiation caused significant epithelial damage and evident opacity in the cornea, together with a local decrease in SOD, catalase and GSH activity. Corneal MDA concentrations increased with UVB exposure. The UVB/Citicoline group had significantly less corneal damage, greater SOD, catalase and GSH activity, and decreased MDA concentrations compared to the UVB only group (p < 0.05). Expression of TGF-β, IL-1β and VEGF was significantly lower in the citicoline/UVB group compared to the UVB group (p < 0.05). Interestingly, TGF-β expression was lower in the citicoline only group compared with controls. Immunfluorescent staining and Western blot analysis showed increased MMP-2, -9 and caspase-3 in the UVB only group compared with the UVB/citicoline group. It was shown that citicoline treatment may be effective in suppressing oxidative stress and controlling inflammation in UVB corneal injury.

Authors+Show Affiliations

Derince Training and Research Hospital, Department of Ophthalmology, Kocaeli, Turkey. Electronic address: drecem@yandex.com.Kocaeli University School of Medicine, Department of Ophthalmology, Kocaeli, Turkey.Kocaeli University School of Medicine, Department of Histology and Embryology, Kocaeli, Turkey.Kocaeli University, Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli, Turkey.Kocaeli University, Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli, Turkey.Kocaeli University School of Medicine, Department of Histology and Embryology, Kocaeli, Turkey; Kocaeli University, Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli, Turkey.Kocaeli University, Ford Otosan Ihsaniye Automotive Vocational School, Department of Motor Vehicles and Transportation Technologies, Kocaeli, Turkey.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33992625

Citation

Tokuc, Ecem Onder, et al. "Protective Effects of Citicoline-containing Eye Drops Against UVB-Induced Corneal Oxidative Damage in a Rat Model." Experimental Eye Research, vol. 208, 2021, p. 108612.
Tokuc EO, Yuksel N, Rencber SF, et al. Protective effects of citicoline-containing eye drops against UVB-Induced corneal oxidative damage in a rat model. Exp Eye Res. 2021;208:108612.
Tokuc, E. O., Yuksel, N., Rencber, S. F., Ozturk, A., Duruksu, G., Yazir, Y., & Ergun, R. E. (2021). Protective effects of citicoline-containing eye drops against UVB-Induced corneal oxidative damage in a rat model. Experimental Eye Research, 208, 108612. https://doi.org/10.1016/j.exer.2021.108612
Tokuc EO, et al. Protective Effects of Citicoline-containing Eye Drops Against UVB-Induced Corneal Oxidative Damage in a Rat Model. Exp Eye Res. 2021;208:108612. PubMed PMID: 33992625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of citicoline-containing eye drops against UVB-Induced corneal oxidative damage in a rat model. AU - Tokuc,Ecem Onder, AU - Yuksel,Nursen, AU - Rencber,Selenay Furat, AU - Ozturk,Ahmet, AU - Duruksu,Gokhan, AU - Yazir,Yusufhan, AU - Ergun,Rıza Emre, Y1 - 2021/05/13/ PY - 2020/08/16/received PY - 2021/04/04/revised PY - 2021/04/29/accepted PY - 2021/5/17/pubmed PY - 2021/9/29/medline PY - 2021/5/16/entrez KW - Citicoline KW - Cornea KW - Oxidative damage KW - UVB SP - 108612 EP - 108612 JF - Experimental eye research JO - Exp Eye Res VL - 208 N2 - It has been reported that citicoline increases antioxidant activity in some tissues. However, the effect of citicoline on corneal wound-healing has not yet been demonstrated. The aim was to investigate the protective effects of citicoline on ultraviolet B (UVB) radiation-induced corneal oxidative damage in a rat model. Four groups (eight animals each) were investigated: controls; UVB only; UVB/citicoline; and citicoline only. Corneal oxidative damage was induced by exposure to UVB radiation at 560 μW/cm2 for five days in the UVB-exposed groups and 1% citicoline eye drops were applied (3xday) for eight days in the two citicoline groups. Corneal surface damage was evaluated by opacity and fluorescein staining. Corneal injury was assessed biochemically by measuring the concentrations of glutathione (GSH) and malondialdehyde (MDA) and the activity of corneal superoxide dismutase (SOD) and catalase. Matrix metalloproteinase (MMP) -2 and -9 and caspase-3 were evaluated by immunofluorescent staining and microscopic examination and by Western blot analysis. Corneal gene expression analysis was performed for vascular endothelial growth factor (VEGF), interleukin-1 beta (IL-1β) and transforming growth factor-beta (TGF-β). UVB radiation caused significant epithelial damage and evident opacity in the cornea, together with a local decrease in SOD, catalase and GSH activity. Corneal MDA concentrations increased with UVB exposure. The UVB/Citicoline group had significantly less corneal damage, greater SOD, catalase and GSH activity, and decreased MDA concentrations compared to the UVB only group (p < 0.05). Expression of TGF-β, IL-1β and VEGF was significantly lower in the citicoline/UVB group compared to the UVB group (p < 0.05). Interestingly, TGF-β expression was lower in the citicoline only group compared with controls. Immunfluorescent staining and Western blot analysis showed increased MMP-2, -9 and caspase-3 in the UVB only group compared with the UVB/citicoline group. It was shown that citicoline treatment may be effective in suppressing oxidative stress and controlling inflammation in UVB corneal injury. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/33992625/Protective_effects_of_citicoline_containing_eye_drops_against_UVB_Induced_corneal_oxidative_damage_in_a_rat_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(21)00178-0 DB - PRIME DP - Unbound Medicine ER -