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Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies.
Int J Nanomedicine. 2021; 16:3343-3356.IJ

Abstract

Background

Abelmoschus esculentus (L.) Moench, an economically important malvaceous vegetable crop popularly known as okra, is used in various culinary preparations and is rich in vitamins, minerals, and nutrients. The biological properties of okra flowers in relation to nanoparticle synthesis have not yet been reported.

Materials and Methods

In the current study, silver nanoparticles (AgNPs) were synthesized using extracts of the flowers of A. esculentus. The characteristics of the AgNPs were studied using a UV-vis spectrometer, Fourier transmission infrared spectrophotometer (FTIR), X-ray diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive X-ray spectrometer (EDX). Antibacterial activity screening was performed using the agar well diffusion method, and cytotoxicity and cell viability studies were conducted using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

Results

The synthesized AgNPs were spherical and ranged in size from 5.52 to 31.96 nm, with an average size of 16.19 nm, as determined by UV-vis spectroscopy, FTIR, XRD, TEM and EDX. A. esculentus flower extract-mediated silver nanoparticles (AME-AgNPs) exhibited excellent activities in vitro studies, particularly in vitro cytotoxic and antiproliferative studies against cancer cell lines, such as the TERT-4 and A-549 cell lines. The antibacterial effects on the Gram-positive pathogens Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes and the Gram-negative pathogens Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium and Shigella sonnei were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values varied with the bacterial strain. The IC50 values of the synthesized NPs for the tested cell lines were close to that of a standard drug.

Conclusion

Compared to other NPs the NPs synthesized in this study were smaller in size and exhibited a higher level of antibacterial activity, cytotoxicity and apoptosis at minimal concentrations, and this is the first study on okra flower-induced anticancer and antimicrobial activities.

Authors+Show Affiliations

Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34017172

Citation

Devanesan, Sandhanasamy, and Mohamad S. AlSalhi. "Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus Esculentus for Cytotoxicity and Antimicrobial Studies." International Journal of Nanomedicine, vol. 16, 2021, pp. 3343-3356.
Devanesan S, AlSalhi MS. Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies. Int J Nanomedicine. 2021;16:3343-3356.
Devanesan, S., & AlSalhi, M. S. (2021). Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies. International Journal of Nanomedicine, 16, 3343-3356. https://doi.org/10.2147/IJN.S307676
Devanesan S, AlSalhi MS. Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus Esculentus for Cytotoxicity and Antimicrobial Studies. Int J Nanomedicine. 2021;16:3343-3356. PubMed PMID: 34017172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies. AU - Devanesan,Sandhanasamy, AU - AlSalhi,Mohamad S, Y1 - 2021/05/14/ PY - 2021/02/24/received PY - 2021/04/24/accepted PY - 2021/5/21/entrez PY - 2021/5/22/pubmed PY - 2021/5/27/medline KW - Abelmoschus esculentus KW - cell lines and cytotoxicity KW - okra green pods KW - silver nanoparticles SP - 3343 EP - 3356 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 16 N2 - Background: Abelmoschus esculentus (L.) Moench, an economically important malvaceous vegetable crop popularly known as okra, is used in various culinary preparations and is rich in vitamins, minerals, and nutrients. The biological properties of okra flowers in relation to nanoparticle synthesis have not yet been reported. Materials and Methods: In the current study, silver nanoparticles (AgNPs) were synthesized using extracts of the flowers of A. esculentus. The characteristics of the AgNPs were studied using a UV-vis spectrometer, Fourier transmission infrared spectrophotometer (FTIR), X-ray diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive X-ray spectrometer (EDX). Antibacterial activity screening was performed using the agar well diffusion method, and cytotoxicity and cell viability studies were conducted using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The synthesized AgNPs were spherical and ranged in size from 5.52 to 31.96 nm, with an average size of 16.19 nm, as determined by UV-vis spectroscopy, FTIR, XRD, TEM and EDX. A. esculentus flower extract-mediated silver nanoparticles (AME-AgNPs) exhibited excellent activities in vitro studies, particularly in vitro cytotoxic and antiproliferative studies against cancer cell lines, such as the TERT-4 and A-549 cell lines. The antibacterial effects on the Gram-positive pathogens Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes and the Gram-negative pathogens Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium and Shigella sonnei were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values varied with the bacterial strain. The IC50 values of the synthesized NPs for the tested cell lines were close to that of a standard drug. Conclusion: Compared to other NPs the NPs synthesized in this study were smaller in size and exhibited a higher level of antibacterial activity, cytotoxicity and apoptosis at minimal concentrations, and this is the first study on okra flower-induced anticancer and antimicrobial activities. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/34017172/Green_Synthesis_of_Silver_Nanoparticles_Using_the_Flower_Extract_of_Abelmoschus_esculentus_for_Cytotoxicity_and_Antimicrobial_Studies_ L2 - https://dx.doi.org/10.2147/IJN.S307676 DB - PRIME DP - Unbound Medicine ER -