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Evaluation of the AGCU Expressmarker 30 Kit composed of 31 loci for forensic application.
Forensic Sci Int. 2021 Jul; 324:110849.FS

Abstract

With the widespread use of STR in identification of individuals, paternity testing, as well as population genetics, many commercially robust and validated STR multiplex kits were developed. The AGCU Expressmarker 30 Kit is a new autosomal STR system that contains 29 autosomal STR loci (D3S1358, vWA, D1S1656, CSF1PO, D8S1132, D19S253, D3S3045, D8S1179, D21S11, D16S539, TPOX, D6S477, Penta D, D2S441, D5S818, TH01, FGA, D15S659, D22S1045, D19S433, D13S317, D7S820, D6S1043, D10S1435, D10S1248, D2S1338, D18S51, D12S391, and Penta E), one insertion/deletion polymorphic marker on the Y chromosome (Y indel), and the amelogenin locus. A series of validation studies were performed in this context according to the guidelines of "Validation Guidelines for Forensic DNA Analysis Methods". The sensitivity study showed that a full profile was observed with template DNA as low as 40 pg. In the stability study, all STR profiles were obtained at concentrations of humic acid up to 800 ng/μL, hematin up to 250 μM, and tannic acid up to 200 ng/μL. The mixture study demonstrated that all of the minor alleles could be called at ratios from 1:1-29:1 when the total DNA was 2 ng. In the population study, the total discrimination power for three population (Sichuan-Han, Gansu-Hui, and Guangxi-Zhuang) were above 0.9999999999999999999999999999999992, 0.999999999999999999999999999999998 and 0.999999999999999999999999999999994 as well as the cumulative probability of paternity exclusion were 0.999999999999953, 0.999999999999178, and 0.999999999999611 respectively. These results demonstrated that the AGCU Expressmarker 30 Kit is a useful tool for analyzing both forensic casework and database samples.

Authors+Show Affiliations

Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.Department of Obstetrics, Xiangya Hospital, Central South University, Changsha 410008, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China. Electronic address: luohaibo@scu.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

34030000

Citation

Zhang, Ke, et al. "Evaluation of the AGCU Expressmarker 30 Kit Composed of 31 Loci for Forensic Application." Forensic Science International, vol. 324, 2021, p. 110849.
Zhang K, Song F, Wang S, et al. Evaluation of the AGCU Expressmarker 30 Kit composed of 31 loci for forensic application. Forensic Sci Int. 2021;324:110849.
Zhang, K., Song, F., Wang, S., Wei, X., Gu, H., Xie, M., Zhou, Y., & Luo, H. (2021). Evaluation of the AGCU Expressmarker 30 Kit composed of 31 loci for forensic application. Forensic Science International, 324, 110849. https://doi.org/10.1016/j.forsciint.2021.110849
Zhang K, et al. Evaluation of the AGCU Expressmarker 30 Kit Composed of 31 Loci for Forensic Application. Forensic Sci Int. 2021;324:110849. PubMed PMID: 34030000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the AGCU Expressmarker 30 Kit composed of 31 loci for forensic application. AU - Zhang,Ke, AU - Song,Feng, AU - Wang,Shuangshuang, AU - Wei,Xiaowen, AU - Gu,Haoyu, AU - Xie,Mingkun, AU - Zhou,Yuxiang, AU - Luo,Haibo, Y1 - 2021/05/19/ PY - 2020/12/28/received PY - 2021/04/26/revised PY - 2021/05/16/accepted PY - 2021/5/25/pubmed PY - 2021/7/22/medline PY - 2021/5/24/entrez KW - AGCU Expressmarker 30 Kit KW - Forensic science KW - STR KW - Validation SP - 110849 EP - 110849 JF - Forensic science international JO - Forensic Sci Int VL - 324 N2 - With the widespread use of STR in identification of individuals, paternity testing, as well as population genetics, many commercially robust and validated STR multiplex kits were developed. The AGCU Expressmarker 30 Kit is a new autosomal STR system that contains 29 autosomal STR loci (D3S1358, vWA, D1S1656, CSF1PO, D8S1132, D19S253, D3S3045, D8S1179, D21S11, D16S539, TPOX, D6S477, Penta D, D2S441, D5S818, TH01, FGA, D15S659, D22S1045, D19S433, D13S317, D7S820, D6S1043, D10S1435, D10S1248, D2S1338, D18S51, D12S391, and Penta E), one insertion/deletion polymorphic marker on the Y chromosome (Y indel), and the amelogenin locus. A series of validation studies were performed in this context according to the guidelines of "Validation Guidelines for Forensic DNA Analysis Methods". The sensitivity study showed that a full profile was observed with template DNA as low as 40 pg. In the stability study, all STR profiles were obtained at concentrations of humic acid up to 800 ng/μL, hematin up to 250 μM, and tannic acid up to 200 ng/μL. The mixture study demonstrated that all of the minor alleles could be called at ratios from 1:1-29:1 when the total DNA was 2 ng. In the population study, the total discrimination power for three population (Sichuan-Han, Gansu-Hui, and Guangxi-Zhuang) were above 0.9999999999999999999999999999999992, 0.999999999999999999999999999999998 and 0.999999999999999999999999999999994 as well as the cumulative probability of paternity exclusion were 0.999999999999953, 0.999999999999178, and 0.999999999999611 respectively. These results demonstrated that the AGCU Expressmarker 30 Kit is a useful tool for analyzing both forensic casework and database samples. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/34030000/Evaluation_of_the_AGCU_Expressmarker_30_Kit_composed_of_31_loci_for_forensic_application_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(21)00169-9 DB - PRIME DP - Unbound Medicine ER -