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CsKTN1 for a katanin p60 subunit is associated with the regulation of fruit elongation in cucumber (Cucumis sativus L.).
Theor Appl Genet. 2021 Aug; 134(8):2429-2441.TA

Abstract

KEY MESSAGE

We identified a short fruit3 (sf3) mutant in cucumber. Map-based cloning revealed that CsKTN1 gene encodes a katanin p60 subunit, which is associated with the regulation of fruit elongation. Fruit length is an important horticultural trait for both fruit yield and quality of cucumber (Cucumis sativus L.). Knowledge on the molecular regulation of fruit elongation in cucumber is very limited. In this study, we identified and characterized a cucumber short fruit3 (sf3) mutant. Histological examination indicated that the shorter fruit in the mutant was due to reduced cell numbers. Genetic analysis revealed that the phenotype of the sf3 mutant was controlled by a single gene with semi-dominant inheritance. By map-based cloning and Arabidopsis genetic transformation, we showed that Sf3 was a homolog of KTN1 (CsKTN1) encoding a katanin p60 subunit. A non-synonymous mutation in the fifth exon of CsKTN1 resulted in an amino acid substitution from Serine in the wild type to Phenylalanine in the sf3 mutant. CsKTN1 expressed in all tissues of both the wild type and the sf3 mutant. However, there was no significant difference in CsKTN1 expression levels between the wild type and the sf3 mutant. The hormone quantitation and RNA-seq analysis suggested that auxin and gibberellin contents are decreased in sf3 by changing the expression levels of genes related with auxin and gibberellin metabolism and signaling. This work helps understand the function of the katanin and the molecular mechanisms of fruit growth regulation in cucumber.

Authors+Show Affiliations

College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.Horticulture Department, USDA-ARS Vegetable Crops Research Unit, University of Wisconsin, Madison, WI, 53706, USA.College of Life Science, Northwest A&F University, Yangling, 712100, Shaanxi, China.Tianjin Vegetable Research Center, Tianjin, 300192, China. National Key Laboratory of Vegetable Germplasm Innovation, Tianjin, 300192, China.Tianjin Vegetable Research Center, Tianjin, 300192, China. waimin163@163.com. National Key Laboratory of Vegetable Germplasm Innovation, Tianjin, 300192, China. waimin163@163.com.College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China. liyuhong73@nwsuaf.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34043036

Citation

Wang, Hui, et al. "CsKTN1 for a Katanin P60 Subunit Is Associated With the Regulation of Fruit Elongation in Cucumber (Cucumis Sativus L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 134, no. 8, 2021, pp. 2429-2441.
Wang H, Sun J, Yang F, et al. CsKTN1 for a katanin p60 subunit is associated with the regulation of fruit elongation in cucumber (Cucumis sativus L.). Theor Appl Genet. 2021;134(8):2429-2441.
Wang, H., Sun, J., Yang, F., Weng, Y., Chen, P., Du, S., Wei, A., & Li, Y. (2021). CsKTN1 for a katanin p60 subunit is associated with the regulation of fruit elongation in cucumber (Cucumis sativus L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 134(8), 2429-2441. https://doi.org/10.1007/s00122-021-03833-y
Wang H, et al. CsKTN1 for a Katanin P60 Subunit Is Associated With the Regulation of Fruit Elongation in Cucumber (Cucumis Sativus L.). Theor Appl Genet. 2021;134(8):2429-2441. PubMed PMID: 34043036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CsKTN1 for a katanin p60 subunit is associated with the regulation of fruit elongation in cucumber (Cucumis sativus L.). AU - Wang,Hui, AU - Sun,Jing, AU - Yang,Fan, AU - Weng,Yiqun, AU - Chen,Peng, AU - Du,Shengli, AU - Wei,Aimin, AU - Li,Yuhong, Y1 - 2021/05/27/ PY - 2020/12/23/received PY - 2021/04/03/accepted PY - 2021/5/28/pubmed PY - 2021/10/1/medline PY - 2021/5/27/entrez SP - 2429 EP - 2441 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 134 IS - 8 N2 - KEY MESSAGE: We identified a short fruit3 (sf3) mutant in cucumber. Map-based cloning revealed that CsKTN1 gene encodes a katanin p60 subunit, which is associated with the regulation of fruit elongation. Fruit length is an important horticultural trait for both fruit yield and quality of cucumber (Cucumis sativus L.). Knowledge on the molecular regulation of fruit elongation in cucumber is very limited. In this study, we identified and characterized a cucumber short fruit3 (sf3) mutant. Histological examination indicated that the shorter fruit in the mutant was due to reduced cell numbers. Genetic analysis revealed that the phenotype of the sf3 mutant was controlled by a single gene with semi-dominant inheritance. By map-based cloning and Arabidopsis genetic transformation, we showed that Sf3 was a homolog of KTN1 (CsKTN1) encoding a katanin p60 subunit. A non-synonymous mutation in the fifth exon of CsKTN1 resulted in an amino acid substitution from Serine in the wild type to Phenylalanine in the sf3 mutant. CsKTN1 expressed in all tissues of both the wild type and the sf3 mutant. However, there was no significant difference in CsKTN1 expression levels between the wild type and the sf3 mutant. The hormone quantitation and RNA-seq analysis suggested that auxin and gibberellin contents are decreased in sf3 by changing the expression levels of genes related with auxin and gibberellin metabolism and signaling. This work helps understand the function of the katanin and the molecular mechanisms of fruit growth regulation in cucumber. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/34043036/CsKTN1_for_a_katanin_p60_subunit_is_associated_with_the_regulation_of_fruit_elongation_in_cucumber__Cucumis_sativus_L___ L2 - https://dx.doi.org/10.1007/s00122-021-03833-y DB - PRIME DP - Unbound Medicine ER -