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Silver Nanoparticle-Anchored Human Hair Kerateine/PEO/PVA Nanofibers for Antibacterial Application and Cell Proliferation.
Molecules. 2021 May 08; 26(9)M

Abstract

The main core of wound treatment is cell growth and anti-infection. To accelerate the proliferation of fibroblasts in the wound and prevent wound infections, various strategies have been tried. It remains a challenge to obtain good cell proliferation and antibacterial effects. Here, human hair kerateine (HHK)/poly(ethylene oxide) (PEO)/poly(vinyl alcohol) (PVA) nanofibers were prepared using cysteine-rich HHK, and then, silver nanoparticles (AgNPs) were in situ anchored in the sulfur-containing amino acid residues of HHK. After the ultrasonic degradation test, HHK/PEO/PVA nanofibrous mats treated with 0.005-M silver nitrate were selected due to their relatively complete structures. It was observed by TEM-EDS that the sulfur-containing amino acids in HHK were the main anchor points of AgNPs. The results of FTIR, XRD and the thermal analysis suggested that the hydrogen bonds between PEO and PVA were broken by HHK and, further, by AgNPs. AgNPs could act as a catalyst to promote the thermal degradation reaction of PVA, PEO and HHK, which was beneficial for silver recycling and medical waste treatment. The antibacterial properties of AgNP-HHK/PEO/PVA nanofibers were examined by the disk diffusion method, and it was observed that they had potential antibacterial capability against Gram-positive bacteria, Gram-negative bacteria and fungi. In addition, HHK in the nanofibrous mats significantly improved the cell proliferation of NIH3T3 cells. These results illustrated that the AgNP-HHK/PEO/PVA nanofibrous mats exhibited excellent antibacterial activity and the ability to promote the proliferation of fibroblasts, reaching our target applications.

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Authors+Show Affiliations

Tang J
Department of Physiology and Hypoxic Biomedicine, Institute of Special Environmental Medicine, Nantong University, Nantong 226019, China. Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
Liu X
Department of Physiology and Hypoxic Biomedicine, Institute of Special Environmental Medicine, Nantong University, Nantong 226019, China. Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
Ge Y
School of Textile and Clothing, Nantong University, Nantong 226019, China. National & Local Joint Engineering Research Center of Technical Fiber Composites for Safety and Protection, Nantong University, Nantong 226019, China.
Wang F
College of Fine Arts and Design, Yangzhou University, Yangzhou 225009, China.

MeSH

AnimalsAnti-Bacterial AgentsCandida albicansCell ProliferationEscherichia coliHairHumansHydrogen BondingKeratins, Hair-SpecificMetal NanoparticlesMiceMicrobial Sensitivity TestsNIH 3T3 CellsNanofibersPolyethylene GlycolsPolyvinyl AlcoholSilverSilver NitrateStaphylococcus aureusWound Healing

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34066875

Citation

Tang, Jiapeng, et al. "Silver Nanoparticle-Anchored Human Hair Kerateine/PEO/PVA Nanofibers for Antibacterial Application and Cell Proliferation." Molecules (Basel, Switzerland), vol. 26, no. 9, 2021.
Tang J, Liu X, Ge Y, et al. Silver Nanoparticle-Anchored Human Hair Kerateine/PEO/PVA Nanofibers for Antibacterial Application and Cell Proliferation. Molecules. 2021;26(9).
Tang, J., Liu, X., Ge, Y., & Wang, F. (2021). Silver Nanoparticle-Anchored Human Hair Kerateine/PEO/PVA Nanofibers for Antibacterial Application and Cell Proliferation. Molecules (Basel, Switzerland), 26(9). https://doi.org/10.3390/molecules26092783
Tang J, et al. Silver Nanoparticle-Anchored Human Hair Kerateine/PEO/PVA Nanofibers for Antibacterial Application and Cell Proliferation. Molecules. 2021 May 8;26(9) PubMed PMID: 34066875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver Nanoparticle-Anchored Human Hair Kerateine/PEO/PVA Nanofibers for Antibacterial Application and Cell Proliferation. AU - Tang,Jiapeng, AU - Liu,Xiwen, AU - Ge,Yan, AU - Wang,Fangfang, Y1 - 2021/05/08/ PY - 2021/04/09/received PY - 2021/05/01/revised PY - 2021/05/06/accepted PY - 2021/6/2/entrez PY - 2021/6/3/pubmed PY - 2021/7/8/medline KW - antibacterial activity KW - cell proliferation KW - kerateine anchoring KW - nanofibers KW - silver nanoparticles JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 9 N2 - The main core of wound treatment is cell growth and anti-infection. To accelerate the proliferation of fibroblasts in the wound and prevent wound infections, various strategies have been tried. It remains a challenge to obtain good cell proliferation and antibacterial effects. Here, human hair kerateine (HHK)/poly(ethylene oxide) (PEO)/poly(vinyl alcohol) (PVA) nanofibers were prepared using cysteine-rich HHK, and then, silver nanoparticles (AgNPs) were in situ anchored in the sulfur-containing amino acid residues of HHK. After the ultrasonic degradation test, HHK/PEO/PVA nanofibrous mats treated with 0.005-M silver nitrate were selected due to their relatively complete structures. It was observed by TEM-EDS that the sulfur-containing amino acids in HHK were the main anchor points of AgNPs. The results of FTIR, XRD and the thermal analysis suggested that the hydrogen bonds between PEO and PVA were broken by HHK and, further, by AgNPs. AgNPs could act as a catalyst to promote the thermal degradation reaction of PVA, PEO and HHK, which was beneficial for silver recycling and medical waste treatment. The antibacterial properties of AgNP-HHK/PEO/PVA nanofibers were examined by the disk diffusion method, and it was observed that they had potential antibacterial capability against Gram-positive bacteria, Gram-negative bacteria and fungi. In addition, HHK in the nanofibrous mats significantly improved the cell proliferation of NIH3T3 cells. These results illustrated that the AgNP-HHK/PEO/PVA nanofibrous mats exhibited excellent antibacterial activity and the ability to promote the proliferation of fibroblasts, reaching our target applications. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/34066875/Silver_Nanoparticle_Anchored_Human_Hair_Kerateine/PEO/PVA_Nanofibers_for_Antibacterial_Application_and_Cell_Proliferation_ DB - PRIME DP - Unbound Medicine ER -