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Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner.
Int J Mol Sci. 2021 May 19; 22(10)IJ

Abstract

In tobacco, the efficiency of Zn translocation to shoots depends on Zn/Cd status. Previous studies pointed to the specific contribution of root parts in the regulation of this process, as well as the role of NtZIP4A/B (from the ZIP family; Zrt Irt-like Proteins). Here, to verify this hypothesis, NtZIP4A/B RNAi lines were generated. Then, in plants exposed to combinations of Zn and Cd concentrations in the medium, the consequences of NtZIP4A/B suppression for the translocation of both metals were determined. Furthermore, the apical, middle, and basal root parts were examined for accumulation of both metals, for Zn localization (using Zinpyr-1), and for modifications of the expression pattern of ZIP genes. Our results confirmed the role of NtZIP4A/B in the control of Zn/Cd-status-dependent transfer of both metals to shoots. Furthermore, they indicated that the middle and basal root parts contributed to the regulation of this process by acting as a reservoir for excess Zn and Cd. Expression studies identified several candidate ZIP genes that interact with NtZIP4A/B in the root in regulating Zn and Cd translocation to the shoot, primarily NtZIP1-like in the basal root part and NtZIP2 in the middle one.

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Authors+Show Affiliations

Maślińska-Gromadka K

Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, 1 Miecznikowa Str., 02-096 Warszawa, Poland.

Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, 1 Miecznikowa Str., 02-096 Warszawa, Poland.

Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, 1 Miecznikowa Str., 02-096 Warszawa, Poland.

Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, 1 Miecznikowa Str., 02-096 Warszawa, Poland.

Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, 1 Miecznikowa Str., 02-096 Warszawa, Poland.

MeSH

Adenosine TriphosphatasesBiological TransportCadmiumCation Transport ProteinsGene Expression Regulation, PlantHomeostasisPlant ProteinsPlant RootsPlant ShootsPlants, Genetically ModifiedTobaccoZinc

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34069632

Citation

Maślińska-Gromadka, Karolina, et al. "Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner." International Journal of Molecular Sciences, vol. 22, no. 10, 2021.

Maślińska-Gromadka K, Barabasz A, Palusińska M, et al. Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner. Int J Mol Sci. 2021;22(10).

Maślińska-Gromadka, K., Barabasz, A., Palusińska, M., Kozak, K., & Antosiewicz, D. M. (2021). Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner. International Journal of Molecular Sciences, 22(10). https://doi.org/10.3390/ijms22105355

Maślińska-Gromadka K, et al. Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner. Int J Mol Sci. 2021 May 19;22(10) PubMed PMID: 34069632.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner. AU - Maślińska-Gromadka,Karolina, AU - Barabasz,Anna, AU - Palusińska,Małgorzata, AU - Kozak,Katarzyna, AU - Antosiewicz,Danuta Maria, Y1 - 2021/05/19/ PY - 2021/03/22/received PY - 2021/04/26/revised PY - 2021/04/28/accepted PY - 2021/6/2/entrez PY - 2021/6/3/pubmed PY - 2021/7/3/medline KW - GUS KW - NtZIP4 KW - ZIP genes KW - Zinpyr-1 KW - cadmium KW - root-to-shoot translocation KW - tobacco KW - zinc JF - International journal of molecular sciences JO - Int J Mol Sci VL - 22 IS - 10 N2 - In tobacco, the efficiency of Zn translocation to shoots depends on Zn/Cd status. Previous studies pointed to the specific contribution of root parts in the regulation of this process, as well as the role of NtZIP4A/B (from the ZIP family; Zrt Irt-like Proteins). Here, to verify this hypothesis, NtZIP4A/B RNAi lines were generated. Then, in plants exposed to combinations of Zn and Cd concentrations in the medium, the consequences of NtZIP4A/B suppression for the translocation of both metals were determined. Furthermore, the apical, middle, and basal root parts were examined for accumulation of both metals, for Zn localization (using Zinpyr-1), and for modifications of the expression pattern of ZIP genes. Our results confirmed the role of NtZIP4A/B in the control of Zn/Cd-status-dependent transfer of both metals to shoots. Furthermore, they indicated that the middle and basal root parts contributed to the regulation of this process by acting as a reservoir for excess Zn and Cd. Expression studies identified several candidate ZIP genes that interact with NtZIP4A/B in the root in regulating Zn and Cd translocation to the shoot, primarily NtZIP1-like in the basal root part and NtZIP2 in the middle one. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/34069632/Suppression_of_NtZIP4A/B_Changes_Zn_and_Cd_Root_to_Shoot_Translocation_in_a_Zn/Cd_Status_Dependent_Manner_ DB - PRIME DP - Unbound Medicine ER -