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Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based Covid-19 Vaccines: Possible Integration into the Human Genome - Are Adenoviral Genes Expressed in Vector-based Vaccines?
Virus Res. 2021 09; 302:198466.VR

Abstract

Vigorous vaccination programs against SARS-CoV-2-causing Covid-19 are the major chance to fight this dreadful pandemic. The currently administered vaccines depend on adenovirus DNA vectors or on SARS-CoV-2 mRNA that might become reverse transcribed into DNA, however infrequently. In some societies, people have become sensitized against the potential short- or long-term side effects of foreign DNA being injected into humans. In my laboratory, the fate of foreign DNA in mammalian (human) cells and organisms has been investigated for many years. In this review, a summary of the results obtained will be presented. This synopsis has been put in the evolutionary context of retrotransposon insertions into pre-human genomes millions of years ago. In addition, studies on adenovirus vector-based DNA, on the fate of food-ingested DNA as well as the long-term persistence of SARS-CoV-2 RNA/DNA will be described. Actual integration of viral DNA molecules and of adenovirus vector DNA will likely be chance events whose frequency and epigenetic consequences cannot with certainty be assessed. The review also addresses problems of remaining adenoviral gene expression in adenoviral-based vectors and their role in side effects of vaccines. Eventually, it will come down to weighing the possible risks of genomic insertions of vaccine-associated foreign DNA and unknown levels of vector-carried adenoviral gene expression versus protection against the dangers of Covid-19. A decision in favor of vaccination against life-threatening disease appears prudent. Informing the public about the complexities of biology will be a reliable guide when having to reach personal decisions about vaccinations.

Authors+Show Affiliations

Institute of Genetics, University of Cologne, D-50674 Cologne, Germany; Institute for Virology, Friedrich-Alexander University (FAU) Erlangen-Nürnberg, D-91054 Erlangen, Germany. Electronic address: walter.doerfler@t-online.de.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

34087261

Citation

Doerfler, Walter. "Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based Covid-19 Vaccines: Possible Integration Into the Human Genome - Are Adenoviral Genes Expressed in Vector-based Vaccines?" Virus Research, vol. 302, 2021, p. 198466.
Doerfler W. Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based Covid-19 Vaccines: Possible Integration into the Human Genome - Are Adenoviral Genes Expressed in Vector-based Vaccines? Virus Res. 2021;302:198466.
Doerfler, W. (2021). Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based Covid-19 Vaccines: Possible Integration into the Human Genome - Are Adenoviral Genes Expressed in Vector-based Vaccines? Virus Research, 302, 198466. https://doi.org/10.1016/j.virusres.2021.198466
Doerfler W. Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based Covid-19 Vaccines: Possible Integration Into the Human Genome - Are Adenoviral Genes Expressed in Vector-based Vaccines. Virus Res. 2021;302:198466. PubMed PMID: 34087261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based Covid-19 Vaccines: Possible Integration into the Human Genome - Are Adenoviral Genes Expressed in Vector-based Vaccines? A1 - Doerfler,Walter, Y1 - 2021/06/01/ PY - 2021/03/24/received PY - 2021/05/10/revised PY - 2021/05/25/accepted PY - 2021/6/5/pubmed PY - 2021/8/10/medline PY - 2021/6/4/entrez KW - Adenovirus DNA integration into mammalian genomes KW - adenovirus vector DNA & RNA-based SARS-CoV-2 vaccines KW - adenovirus vector DNA integration into human genome KW - epigenetic consequences of foreign DNA integration KW - expression of adenoviral genes in vector DNA KW - retrotranscription of SARS-CoV-2 RNA into DNA SP - 198466 EP - 198466 JF - Virus research JO - Virus Res VL - 302 N2 - Vigorous vaccination programs against SARS-CoV-2-causing Covid-19 are the major chance to fight this dreadful pandemic. The currently administered vaccines depend on adenovirus DNA vectors or on SARS-CoV-2 mRNA that might become reverse transcribed into DNA, however infrequently. In some societies, people have become sensitized against the potential short- or long-term side effects of foreign DNA being injected into humans. In my laboratory, the fate of foreign DNA in mammalian (human) cells and organisms has been investigated for many years. In this review, a summary of the results obtained will be presented. This synopsis has been put in the evolutionary context of retrotransposon insertions into pre-human genomes millions of years ago. In addition, studies on adenovirus vector-based DNA, on the fate of food-ingested DNA as well as the long-term persistence of SARS-CoV-2 RNA/DNA will be described. Actual integration of viral DNA molecules and of adenovirus vector DNA will likely be chance events whose frequency and epigenetic consequences cannot with certainty be assessed. The review also addresses problems of remaining adenoviral gene expression in adenoviral-based vectors and their role in side effects of vaccines. Eventually, it will come down to weighing the possible risks of genomic insertions of vaccine-associated foreign DNA and unknown levels of vector-carried adenoviral gene expression versus protection against the dangers of Covid-19. A decision in favor of vaccination against life-threatening disease appears prudent. Informing the public about the complexities of biology will be a reliable guide when having to reach personal decisions about vaccinations. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/34087261/Adenoviral_Vector_DNA__and_SARS_CoV_2_mRNA_Based_Covid_19_Vaccines:_Possible_Integration_into_the_Human_Genome___Are_Adenoviral_Genes_Expressed_in_Vector_based_Vaccines L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(21)00173-8 DB - PRIME DP - Unbound Medicine ER -