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Comparative uptake of exogenous thiamine and subsequent metabolic footprint in Saccharomyces cerevisiae and Kluyveromyces marxianus under simulated oenological conditions.
Int J Food Microbiol. 2021 Sep 16; 354:109206.IJ

Abstract

Managed inoculation of non-Saccharomyces yeast species is regarded as a practical way to introduce new characteristics to wine. However, these yeasts struggle to survive until fermentation is complete. Kluyveromyces marxianus IWBT Y885 is one such yeast. Although it displays interesting oenological properties, a longer persistence during alcoholic fermentation would warranty a stronger impact on wine composition. A key factor for survival, growth and sustained metabolic activity of all yeasts is their nutrient requirements. Thus, identifying nutrients that are essential for maximising fermentation performance, and subsequently ensuring adequate levels of nutrients, is a means to ensure significant contribution of yeasts to wine properties. This study aimed to identify essential nutrients, other than previously studied sugars and nitrogen, for maximum impact of K. marxianus Y885, as well as to characterise the outcomes of their utilisation. A multifactorial experimental design was employed to investigate the impact of nutrient concentrations on fermentation performance with K. marxianus Y885 in synthetic must. B-complex vitamins most significantly impacted fermentation performance of K. marxianus Y885 compared to other nutrient groups investigated. Considering the well-established role of the vitamin, thiamine, for maximum fermentation performance during winemaking and the fact that it may be supplemented to wine fermentations legally, the responses to specifically exogenous thiamine concentration for K. marxianus Y885 and Saccharomyces cerevisiae EC1118 were compared in terms of population viability, fermentation rate, total sugars utilised, thiamine assimilation kinetics, and final wine composition. A saturation effect for initial thiamine concentration of K. marxianus Y885 fermentations was characterised, with a maximum fermentation rate and over 90% of available sugars utilisation obtained at 0.25 mg/L. An appreciably larger comparative increase in exponential cell growth rate, maximum population, fermentation rate and total CO2 production for K. marxianus Y885 compared to S. cerevisiae EC1118 revealed a greater necessity for thiamine to ensure maximum fermentation performance. A delayed uptake of thiamine at higher concentrations for K. marxianus Y885 suggested differential regulation of thiamine uptake compared to S. cerevisiae EC1118. In addition, different trends in metabolites produced between species suggest that thiamine concentration impacts the carbon metabolic flux differently in these two yeasts, potentially impacting final wine properties.

Authors+Show Affiliations

South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Private Bag X1, Matieland 7602, South Africa.South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Private Bag X1, Matieland 7602, South Africa.South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Private Bag X1, Matieland 7602, South Africa. Electronic address: divol@sun.ac.za.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34088559

Citation

Labuschagne, Pieter W J., et al. "Comparative Uptake of Exogenous Thiamine and Subsequent Metabolic Footprint in Saccharomyces Cerevisiae and Kluyveromyces Marxianus Under Simulated Oenological Conditions." International Journal of Food Microbiology, vol. 354, 2021, p. 109206.
Labuschagne PWJ, Rollero S, Divol B. Comparative uptake of exogenous thiamine and subsequent metabolic footprint in Saccharomyces cerevisiae and Kluyveromyces marxianus under simulated oenological conditions. Int J Food Microbiol. 2021;354:109206.
Labuschagne, P. W. J., Rollero, S., & Divol, B. (2021). Comparative uptake of exogenous thiamine and subsequent metabolic footprint in Saccharomyces cerevisiae and Kluyveromyces marxianus under simulated oenological conditions. International Journal of Food Microbiology, 354, 109206. https://doi.org/10.1016/j.ijfoodmicro.2021.109206
Labuschagne PWJ, Rollero S, Divol B. Comparative Uptake of Exogenous Thiamine and Subsequent Metabolic Footprint in Saccharomyces Cerevisiae and Kluyveromyces Marxianus Under Simulated Oenological Conditions. Int J Food Microbiol. 2021 Sep 16;354:109206. PubMed PMID: 34088559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative uptake of exogenous thiamine and subsequent metabolic footprint in Saccharomyces cerevisiae and Kluyveromyces marxianus under simulated oenological conditions. AU - Labuschagne,Pieter W J, AU - Rollero,Stéphanie, AU - Divol,Benoit, Y1 - 2021/04/21/ PY - 2020/10/28/received PY - 2021/04/12/revised PY - 2021/04/13/accepted PY - 2021/6/6/pubmed PY - 2021/9/25/medline PY - 2021/6/5/entrez KW - Kluyveromyces marxianus KW - Saccharomyces cerevisiae KW - Thiamine KW - Wine alcoholic fermentation SP - 109206 EP - 109206 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 354 N2 - Managed inoculation of non-Saccharomyces yeast species is regarded as a practical way to introduce new characteristics to wine. However, these yeasts struggle to survive until fermentation is complete. Kluyveromyces marxianus IWBT Y885 is one such yeast. Although it displays interesting oenological properties, a longer persistence during alcoholic fermentation would warranty a stronger impact on wine composition. A key factor for survival, growth and sustained metabolic activity of all yeasts is their nutrient requirements. Thus, identifying nutrients that are essential for maximising fermentation performance, and subsequently ensuring adequate levels of nutrients, is a means to ensure significant contribution of yeasts to wine properties. This study aimed to identify essential nutrients, other than previously studied sugars and nitrogen, for maximum impact of K. marxianus Y885, as well as to characterise the outcomes of their utilisation. A multifactorial experimental design was employed to investigate the impact of nutrient concentrations on fermentation performance with K. marxianus Y885 in synthetic must. B-complex vitamins most significantly impacted fermentation performance of K. marxianus Y885 compared to other nutrient groups investigated. Considering the well-established role of the vitamin, thiamine, for maximum fermentation performance during winemaking and the fact that it may be supplemented to wine fermentations legally, the responses to specifically exogenous thiamine concentration for K. marxianus Y885 and Saccharomyces cerevisiae EC1118 were compared in terms of population viability, fermentation rate, total sugars utilised, thiamine assimilation kinetics, and final wine composition. A saturation effect for initial thiamine concentration of K. marxianus Y885 fermentations was characterised, with a maximum fermentation rate and over 90% of available sugars utilisation obtained at 0.25 mg/L. An appreciably larger comparative increase in exponential cell growth rate, maximum population, fermentation rate and total CO2 production for K. marxianus Y885 compared to S. cerevisiae EC1118 revealed a greater necessity for thiamine to ensure maximum fermentation performance. A delayed uptake of thiamine at higher concentrations for K. marxianus Y885 suggested differential regulation of thiamine uptake compared to S. cerevisiae EC1118. In addition, different trends in metabolites produced between species suggest that thiamine concentration impacts the carbon metabolic flux differently in these two yeasts, potentially impacting final wine properties. SN - 1879-3460 UR - https://www.unboundmedicine.com/medline/citation/34088559/Comparative_uptake_of_exogenous_thiamine_and_subsequent_metabolic_footprint_in_Saccharomyces_cerevisiae_and_Kluyveromyces_marxianus_under_simulated_oenological_conditions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(21)00165-3 DB - PRIME DP - Unbound Medicine ER -