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Identification of a recessive gene YrZ15-1370 conferring adult plant resistance to stripe rust in wheat-Triticum boeoticum introgression line.
Theor Appl Genet. 2021 Sep; 134(9):2891-2900.TA

Abstract

KEY MESSAGE

A novel recessive gene YrZ15-1370 derived from Triticum boeoticum confers adult-plant resistance to wheat stripe rust. Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat globally and resistance is the effectively control strategy. Triticum boeoticum Boiss (T. monococcum L. ssp. aegilopoides, 2n = 2x = 14, AbAb) accession G52 confers a high level of adult-plant resistance against a mixture of the Chinese prevalent Pst races. To transfer the resistance to common wheat, a cross was made between G52 and susceptible common wheat genotype Crocus. A highly resistant wheat-T. boeoticum introgression line Z15-1370 (F5 generation) with 42 chromosomes was selected cytologically and by testing with Pst races. F1, F2, and F2:3 generations of the cross between Z15-1370 and stripe rust susceptible common wheat Mingxian169 were developed. Genetic analysis revealed that the resistance in Z15-1370 was controlled by a single recessive gene, tentatively designated YrZ15-1370. Using the bulked segregant RNA-Seq (BSR-Seq) analysis, YrZ15-1370 was mapped to chromosome 6AL and flanked by markers KASP1370-3 and KASP-1370-5 within a 4.3 cM genetic interval corresponding to 1.8 Mb physical region in the Chinese Spring genome, in which a number of disease resistance-related genes were annotated. YrZ15-1370 differed from previously Yr genes identified on chromosome 6A based on its position and/or origin. The YrZ15-1370 would be a valuable resource for wheat resistance improvement and the flanking markers developed here could be useful tools for marker-assisted selection (MAS) in breeding and further cloning the gene.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China. lhuang@sicau.edu.cn. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China. lhuang@sicau.edu.cn.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China. zhanglianquan1977@126.com. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China. zhanglianquan1977@126.com.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, China.

MeSH

BasidiomycotaDisease ResistanceGene Expression Regulation, PlantGenes, RecessivePlant BreedingPlant DiseasesPlant ProteinsRNA-SeqSeasonsTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34089337

Citation

Zhang, Minghu, et al. "Identification of a Recessive Gene YrZ15-1370 Conferring Adult Plant Resistance to Stripe Rust in wheat-Triticum Boeoticum Introgression Line." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 134, no. 9, 2021, pp. 2891-2900.

Zhang M, Liu X, Peng T, et al. Identification of a recessive gene YrZ15-1370 conferring adult plant resistance to stripe rust in wheat-Triticum boeoticum introgression line. Theor Appl Genet. 2021;134(9):2891-2900.

Zhang, M., Liu, X., Peng, T., Wang, D., Liang, D., Li, H., Hao, M., Ning, S., Yuan, Z., Jiang, B., Chen, X., Chen, X., Huang, L., Zhang, L., & Liu, D. (2021). Identification of a recessive gene YrZ15-1370 conferring adult plant resistance to stripe rust in wheat-Triticum boeoticum introgression line. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 134(9), 2891-2900. https://doi.org/10.1007/s00122-021-03866-3

Zhang M, et al. Identification of a Recessive Gene YrZ15-1370 Conferring Adult Plant Resistance to Stripe Rust in wheat-Triticum Boeoticum Introgression Line. Theor Appl Genet. 2021;134(9):2891-2900. PubMed PMID: 34089337.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Identification of a recessive gene YrZ15-1370 conferring adult plant resistance to stripe rust in wheat-Triticum boeoticum introgression line. AU - Zhang,Minghu, AU - Liu,Xin, AU - Peng,Ting, AU - Wang,Dinghao, AU - Liang,Dongyu, AU - Li,Hongyu, AU - Hao,Ming, AU - Ning,Shunzong, AU - Yuan,Zhongwei, AU - Jiang,Bo, AU - Chen,Xuejiao, AU - Chen,Xue, AU - Huang,Lin, AU - Zhang,Lianquan, AU - Liu,Dengcai, Y1 - 2021/06/05/ PY - 2021/02/25/received PY - 2021/05/17/accepted PY - 2021/6/6/pubmed PY - 2021/10/2/medline PY - 2021/6/5/entrez SP - 2891 EP - 2900 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 134 IS - 9 N2 - KEY MESSAGE: A novel recessive gene YrZ15-1370 derived from Triticum boeoticum confers adult-plant resistance to wheat stripe rust. Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat globally and resistance is the effectively control strategy. Triticum boeoticum Boiss (T. monococcum L. ssp. aegilopoides, 2n = 2x = 14, AbAb) accession G52 confers a high level of adult-plant resistance against a mixture of the Chinese prevalent Pst races. To transfer the resistance to common wheat, a cross was made between G52 and susceptible common wheat genotype Crocus. A highly resistant wheat-T. boeoticum introgression line Z15-1370 (F5 generation) with 42 chromosomes was selected cytologically and by testing with Pst races. F1, F2, and F2:3 generations of the cross between Z15-1370 and stripe rust susceptible common wheat Mingxian169 were developed. Genetic analysis revealed that the resistance in Z15-1370 was controlled by a single recessive gene, tentatively designated YrZ15-1370. Using the bulked segregant RNA-Seq (BSR-Seq) analysis, YrZ15-1370 was mapped to chromosome 6AL and flanked by markers KASP1370-3 and KASP-1370-5 within a 4.3 cM genetic interval corresponding to 1.8 Mb physical region in the Chinese Spring genome, in which a number of disease resistance-related genes were annotated. YrZ15-1370 differed from previously Yr genes identified on chromosome 6A based on its position and/or origin. The YrZ15-1370 would be a valuable resource for wheat resistance improvement and the flanking markers developed here could be useful tools for marker-assisted selection (MAS) in breeding and further cloning the gene. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/34089337/Identification_of_a_recessive_gene_YrZ15_1370_conferring_adult_plant_resistance_to_stripe_rust_in_wheat_Triticum_boeoticum_introgression_line_ DB - PRIME DP - Unbound Medicine ER -