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Enhanced electrochemiluminescence ratiometric cytosensing based on surface plasmon resonance of Au nanoparticles and nanosucculent films.
Biosens Bioelectron. 2021 Oct 01; 189:113367.BB

Abstract

Ramos cells are human Burkitt's lymphoma cells, which are a kind of cancer cells to facilitate the monitoring of the relevant biological processes of cancers. Sensitive and accurate detection of Ramos cells using emerging ratiometric ECL biosensing technology shows increasing importance, however, the target analytes of current ratiometric ECL biosensors are mainly limited to DNA/RNA or proteins. In this study, we proposed a dual-potential ratiometric sensing strategy for the electrochemiluminescence detection of Ramos cells based on two types of electrochemiluminescence (ECL)-responding molecular. Au nanosucculent films (AuNFs) were electrodeposited on the fluorine doped tin oxide (FTO) electrode to increase the effective area of the electrode for more efficient assembly of DNA and effectively improving the conductivity of the sensing interfaces. In the presence of Ramos cells, aptamers capped with Au@luminol would conjugate with Ramos cells and then remove from AuNFs, accompanying the decrease of ECL signal from Au@luminol. Then, Au-DNA was captured and alternately hybridized with DNA-modified CdS nanocrystals (NCs) on the surface of AuNFs with the formation of a super reticulate structure. The reticulate structure not only raised another identified ECL signal from CdS NCs but also greatly promoted its ECL intensity from the surface plasmon resonance originating from Au NPs. The value of log (ECLCdS/ECLluminol) and the logarithm of the number of cells exhibit considerable linear relation ranging from 80 to 8 × 105 cells mL-1 with a low detection limit of 20 cells mL-1 (S/N = 3). The selectivity and specificity of this dual-potential ECL sensor showed good performance and indicated considerable promise in avoiding false-positive results in detection.

Authors+Show Affiliations

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China. Electronic address: zhouhong@qust.edu.cn.Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong, 266071, PR China.The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266071, PR China.College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, Shandong, 266590, PR China. Electronic address: jingliu@sdust.edu.cn.Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong, 266071, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34091285

Citation

Zhou, Hong, et al. "Enhanced Electrochemiluminescence Ratiometric Cytosensing Based On Surface Plasmon Resonance of Au Nanoparticles and Nanosucculent Films." Biosensors & Bioelectronics, vol. 189, 2021, p. 113367.
Zhou H, Ding K, Yu Q, et al. Enhanced electrochemiluminescence ratiometric cytosensing based on surface plasmon resonance of Au nanoparticles and nanosucculent films. Biosens Bioelectron. 2021;189:113367.
Zhou, H., Ding, K., Yu, Q., Wang, H., Liu, J., & Wang, Z. (2021). Enhanced electrochemiluminescence ratiometric cytosensing based on surface plasmon resonance of Au nanoparticles and nanosucculent films. Biosensors & Bioelectronics, 189, 113367. https://doi.org/10.1016/j.bios.2021.113367
Zhou H, et al. Enhanced Electrochemiluminescence Ratiometric Cytosensing Based On Surface Plasmon Resonance of Au Nanoparticles and Nanosucculent Films. Biosens Bioelectron. 2021 Oct 1;189:113367. PubMed PMID: 34091285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced electrochemiluminescence ratiometric cytosensing based on surface plasmon resonance of Au nanoparticles and nanosucculent films. AU - Zhou,Hong, AU - Ding,Kexin, AU - Yu,Qiao, AU - Wang,Haiyan, AU - Liu,Jing, AU - Wang,Zonghua, Y1 - 2021/06/01/ PY - 2021/04/12/received PY - 2021/05/20/revised PY - 2021/05/23/accepted PY - 2021/6/7/pubmed PY - 2021/6/22/medline PY - 2021/6/6/entrez KW - Au nanosucculent films KW - Au@luminol nanocomposite KW - CdS nanocrystals KW - Electrochemiluminescence KW - Ratiometric biosensor KW - Surface plasmon resonance SP - 113367 EP - 113367 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 189 N2 - Ramos cells are human Burkitt's lymphoma cells, which are a kind of cancer cells to facilitate the monitoring of the relevant biological processes of cancers. Sensitive and accurate detection of Ramos cells using emerging ratiometric ECL biosensing technology shows increasing importance, however, the target analytes of current ratiometric ECL biosensors are mainly limited to DNA/RNA or proteins. In this study, we proposed a dual-potential ratiometric sensing strategy for the electrochemiluminescence detection of Ramos cells based on two types of electrochemiluminescence (ECL)-responding molecular. Au nanosucculent films (AuNFs) were electrodeposited on the fluorine doped tin oxide (FTO) electrode to increase the effective area of the electrode for more efficient assembly of DNA and effectively improving the conductivity of the sensing interfaces. In the presence of Ramos cells, aptamers capped with Au@luminol would conjugate with Ramos cells and then remove from AuNFs, accompanying the decrease of ECL signal from Au@luminol. Then, Au-DNA was captured and alternately hybridized with DNA-modified CdS nanocrystals (NCs) on the surface of AuNFs with the formation of a super reticulate structure. The reticulate structure not only raised another identified ECL signal from CdS NCs but also greatly promoted its ECL intensity from the surface plasmon resonance originating from Au NPs. The value of log (ECLCdS/ECLluminol) and the logarithm of the number of cells exhibit considerable linear relation ranging from 80 to 8 × 105 cells mL-1 with a low detection limit of 20 cells mL-1 (S/N = 3). The selectivity and specificity of this dual-potential ECL sensor showed good performance and indicated considerable promise in avoiding false-positive results in detection. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/34091285/Enhanced_electrochemiluminescence_ratiometric_cytosensing_based_on_surface_plasmon_resonance_of_Au_nanoparticles_and_nanosucculent_films_ DB - PRIME DP - Unbound Medicine ER -