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A mutation in CsHY2 encoding a phytochromobilin (PΦB) synthase leads to an elongated hypocotyl 1(elh1) phenotype in cucumber (Cucumis sativus L.).
Theor Appl Genet. 2021 Aug; 134(8):2639-2652.TA

Abstract

KEY MESSAGE

The elongated hypocotyl1 (elh1) mutant in cucumber is due to a mutation in CsHY2, which is a homolog of the Arabidopsis HY2 encoding the phytochromobilin (PΦB) synthase for phytochrome biosynthesis Hypocotyl length is a critical determinant in establishing high quality seedlings for successful cucumber production, but knowledge on the molecular regulation of hypocotyl growth in cucumber is very limited. Here, we reported identification and characterization of a cucumber elongated hypocotyl 1 (elh1) mutant. We found that the longer hypocotyl in elh1 was due to longitudinal growth of hypocotyl cells. With fine mapping, the elh1 locus was delimited to a 20.9-kb region containing three annotated genes; only one polymorphism was identified in this region between two parental lines, which was a non-synonymous SNP (G28153633A) in the third exon of CsHY2 (CsGy1G030000) that encodes a phytochromobilin (PΦB) synthase. Uniqueness of the mutant allele at CsHY2 was verified in natural cucumber populations. Ectopic expression of CsHY2 in Arabidopsis hy2-1 long-hypocotyl mutant led to reduced hypocotyl length. The PΦB protein was targeted to the chloroplast. The expression levels of CsHY2 and five phytochrome genes CsPHYA1, CsPHYA2, CsPHYB, CsPHYC and CsPHYE were all significantly down-regulated while several cell elongation related genes were up-regulated in elh1 mutant compared to wild-type cucumber, which are correlated with dynamic hypocotyl elongation in the mutant. RNA-seq analysis in the WT and mutant revealed differentially expressed genes involved in porphyrin and chlorophyll metabolisms, cell elongation and plant hormone signal transduction pathways. This is the first report to characterize and clone the CsHY2 gene in cucumber. This work reveals the important of CsHY2 in regulating hypocotyl length and extends our understanding of the roles of CsHY2 in cucumber.

Authors+Show Affiliations

College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.Horticulture Department, USDA-ARS Vegetable Crops Research Unit, University of Wisconsin, Madison, WI, 53706, USA.College of Life Science, Northwest A & F University, Yangling, 712100, Shaanxi,, China.Tianjin Vegetable Research Center, Tianjin, 300192, China. National Key Laboratory of Vegetable Germplasm Innovation, Tianjin, 300192, China.Tianjin Vegetable Research Center, Tianjin, 300192, China. waimin163@163.com. National Key Laboratory of Vegetable Germplasm Innovation, Tianjin, 300192, China. waimin163@163.com.College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China. liyuhong73@nwsuaf.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34091695

Citation

Hu, Liangliang, et al. "A Mutation in CsHY2 Encoding a Phytochromobilin (PΦB) Synthase Leads to an Elongated Hypocotyl 1(elh1) Phenotype in Cucumber (Cucumis Sativus L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 134, no. 8, 2021, pp. 2639-2652.
Hu L, Liu P, Jin Z, et al. A mutation in CsHY2 encoding a phytochromobilin (PΦB) synthase leads to an elongated hypocotyl 1(elh1) phenotype in cucumber (Cucumis sativus L.). Theor Appl Genet. 2021;134(8):2639-2652.
Hu, L., Liu, P., Jin, Z., Sun, J., Weng, Y., Chen, P., Du, S., Wei, A., & Li, Y. (2021). A mutation in CsHY2 encoding a phytochromobilin (PΦB) synthase leads to an elongated hypocotyl 1(elh1) phenotype in cucumber (Cucumis sativus L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 134(8), 2639-2652. https://doi.org/10.1007/s00122-021-03849-4
Hu L, et al. A Mutation in CsHY2 Encoding a Phytochromobilin (PΦB) Synthase Leads to an Elongated Hypocotyl 1(elh1) Phenotype in Cucumber (Cucumis Sativus L.). Theor Appl Genet. 2021;134(8):2639-2652. PubMed PMID: 34091695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A mutation in CsHY2 encoding a phytochromobilin (PΦB) synthase leads to an elongated hypocotyl 1(elh1) phenotype in cucumber (Cucumis sativus L.). AU - Hu,Liangliang, AU - Liu,Peng, AU - Jin,Zhuoshuai, AU - Sun,Jing, AU - Weng,Yiqun, AU - Chen,Peng, AU - Du,Shengli, AU - Wei,Aimin, AU - Li,Yuhong, Y1 - 2021/06/06/ PY - 2021/01/21/received PY - 2021/04/29/accepted PY - 2021/6/7/pubmed PY - 2021/10/1/medline PY - 2021/6/6/entrez SP - 2639 EP - 2652 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 134 IS - 8 N2 - KEY MESSAGE: The elongated hypocotyl1 (elh1) mutant in cucumber is due to a mutation in CsHY2, which is a homolog of the Arabidopsis HY2 encoding the phytochromobilin (PΦB) synthase for phytochrome biosynthesis Hypocotyl length is a critical determinant in establishing high quality seedlings for successful cucumber production, but knowledge on the molecular regulation of hypocotyl growth in cucumber is very limited. Here, we reported identification and characterization of a cucumber elongated hypocotyl 1 (elh1) mutant. We found that the longer hypocotyl in elh1 was due to longitudinal growth of hypocotyl cells. With fine mapping, the elh1 locus was delimited to a 20.9-kb region containing three annotated genes; only one polymorphism was identified in this region between two parental lines, which was a non-synonymous SNP (G28153633A) in the third exon of CsHY2 (CsGy1G030000) that encodes a phytochromobilin (PΦB) synthase. Uniqueness of the mutant allele at CsHY2 was verified in natural cucumber populations. Ectopic expression of CsHY2 in Arabidopsis hy2-1 long-hypocotyl mutant led to reduced hypocotyl length. The PΦB protein was targeted to the chloroplast. The expression levels of CsHY2 and five phytochrome genes CsPHYA1, CsPHYA2, CsPHYB, CsPHYC and CsPHYE were all significantly down-regulated while several cell elongation related genes were up-regulated in elh1 mutant compared to wild-type cucumber, which are correlated with dynamic hypocotyl elongation in the mutant. RNA-seq analysis in the WT and mutant revealed differentially expressed genes involved in porphyrin and chlorophyll metabolisms, cell elongation and plant hormone signal transduction pathways. This is the first report to characterize and clone the CsHY2 gene in cucumber. This work reveals the important of CsHY2 in regulating hypocotyl length and extends our understanding of the roles of CsHY2 in cucumber. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/34091695/A_mutation_in_CsHY2_encoding_a_phytochromobilin__PΦB__synthase_leads_to_an_elongated_hypocotyl_1_elh1__phenotype_in_cucumber__Cucumis_sativus_L___ L2 - https://dx.doi.org/10.1007/s00122-021-03849-4 DB - PRIME DP - Unbound Medicine ER -