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Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity.
BMC Biotechnol. 2021 06 16; 21(1):40.BB

Abstract

BACKGROUND

Most commercial phycocyanins are extracted from a filamentous cyanobacterium, Arthrospira (Spirulina) platensis. Owing to the expenses of culture and complexities of the physical and chemical methods of phycocyanin purification, a more effective and simple method is required.

RESULTS

We developed a new method for efficiently recovering the blue pigment protein, phycocyanin, from unique filamentous cyanobacteria, Pseudanabaena sp. ABRG5-3 and Limnothrix sp. SK1-2-1. The cells were cultivated in economy medium BG11 and lysed by adding water in a 1:16 ratio of wet cells to water. After extraction and purification, 28-30% dry cell weight of phycocyanin was obtained and its purity was confirmed. The stabilities of the phycocyanins at different pH in the presence of high temperature and light conditions and their antioxidant abilities were assessed. Results indicated that the phycocyanins were stable and possessed antioxidant properties. Interestingly, the Pseudanabaena phycocyanin was less likely to deteriorate under acidic conditions.

CONCLUSIONS

Overall, we developed a promising and novel method for producing high functional phycocyanin concentrations at a low cost. The possibilities of adapting this new phycocyanin biorefinery to unique bioreactor utilization have also been discussed.

Links

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Authors+Show Affiliations

Aoki J
College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu-shi, Tokyo 183-8509, Japan.
Sasaki D
BioX Chemical Industries Co. Ltd., 2-20-11 Inokuchidai, Nishi-ku, Hiroshima 733-0844, Japan.
Asayama M
College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan. munehiko.asayama.777@vc.ibaraki.ac.jp. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu-shi, Tokyo 183-8509, Japan. munehiko.asayama.777@vc.ibaraki.ac.jp.

MeSH

AntioxidantsChemical FractionationHot TemperatureHydrogen-Ion ConcentrationPhycocyaninSpirulina

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34134665

Citation

Aoki, Jinichi, et al. "Development of a Method for Phycocyanin Recovery From Filamentous Cyanobacteria and Evaluation of Its Stability and Antioxidant Capacity." BMC Biotechnology, vol. 21, no. 1, 2021, p. 40.
Aoki J, Sasaki D, Asayama M. Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity. BMC Biotechnol. 2021;21(1):40.
Aoki, J., Sasaki, D., & Asayama, M. (2021). Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity. BMC Biotechnology, 21(1), 40. https://doi.org/10.1186/s12896-021-00692-9
Aoki J, Sasaki D, Asayama M. Development of a Method for Phycocyanin Recovery From Filamentous Cyanobacteria and Evaluation of Its Stability and Antioxidant Capacity. BMC Biotechnol. 2021 06 16;21(1):40. PubMed PMID: 34134665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity. AU - Aoki,Jinichi, AU - Sasaki,Daisaku, AU - Asayama,Munehiko, Y1 - 2021/06/16/ PY - 2020/12/29/received PY - 2021/04/12/accepted PY - 2021/6/17/entrez PY - 2021/6/18/pubmed PY - 2021/12/29/medline KW - Antioxidant KW - Arthrospira platensis KW - Cell lysis KW - Limnothrix sp. KW - Phycocyanin KW - Pseudanabaena sp. SP - 40 EP - 40 JF - BMC biotechnology JO - BMC Biotechnol VL - 21 IS - 1 N2 - BACKGROUND: Most commercial phycocyanins are extracted from a filamentous cyanobacterium, Arthrospira (Spirulina) platensis. Owing to the expenses of culture and complexities of the physical and chemical methods of phycocyanin purification, a more effective and simple method is required. RESULTS: We developed a new method for efficiently recovering the blue pigment protein, phycocyanin, from unique filamentous cyanobacteria, Pseudanabaena sp. ABRG5-3 and Limnothrix sp. SK1-2-1. The cells were cultivated in economy medium BG11 and lysed by adding water in a 1:16 ratio of wet cells to water. After extraction and purification, 28-30% dry cell weight of phycocyanin was obtained and its purity was confirmed. The stabilities of the phycocyanins at different pH in the presence of high temperature and light conditions and their antioxidant abilities were assessed. Results indicated that the phycocyanins were stable and possessed antioxidant properties. Interestingly, the Pseudanabaena phycocyanin was less likely to deteriorate under acidic conditions. CONCLUSIONS: Overall, we developed a promising and novel method for producing high functional phycocyanin concentrations at a low cost. The possibilities of adapting this new phycocyanin biorefinery to unique bioreactor utilization have also been discussed. SN - 1472-6750 UR - https://www.unboundmedicine.com/medline/citation/34134665/Development_of_a_method_for_phycocyanin_recovery_from_filamentous_cyanobacteria_and_evaluation_of_its_stability_and_antioxidant_capacity_ DB - PRIME DP - Unbound Medicine ER -