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Chitosan nanoparticles alleviated endocrine disruption, oxidative damage, and genotoxicity of Bisphenol-A- intoxicated female African catfish.

Abstract

Bisphenol-A (BPA) is widely used in production of plastic products. It can reach the ecosystems affecting aquatic organisms most likely fishes. The purpose of this study was to study the toxic effects of BPA on the biochemical variables and oxidative stress in female African catfish, Clarias gariepinus and to estimate the protective role of chitosan nanoparticles (CSNPs) against BPA toxicity. Five groups in triplicates of fish were divided as follows: group I was control, group II was treated with CSNPs (0.66 ml/L), group III was exposed to BPA (1.43 μg/L), group IV was treated with BPA (1.43 μg/L) plus CSNPs (0.33 ml/L), and group V was treated with BPA (1.43 μg/L) plus CSNPs (0.66 ml/L) for 30 days. Blood and liver tissue samples were collected at the end of experiment for the biochemical and oxidative stress biomarkers analyses. Results exhibited that serum Follicle Stimulating Hormone (FSH) and 17-β Estradiol (E2) were significantly decreased in female catfish. While, serum Testosterone (T.) and Luteinizing Hormone (LH) were increased after exposure to BPA. Marked increment in superoxide dismutase (SOD) and malondialdehyde (MDA) levels of hepatic tissue of catfish exposed to BPA. Furthermore, significant reduction in hepatic catalase (CAT), glutathione peroxidase (GSH-px), total antioxidant capacity (TAC), reduced glutathione (GSH), and glutathione S-transferase (GST) levels were decreased significantly in BPA-exposed catfish compared to the control group. However, administration of female C. gariepinus with the low and high doses (0.33 ml/L and 0.66 ml/L) of CNPs restored the biochemical parameters to be close to the normal values of the control group and also, reduced oxidative stress induced by BPA toxicity. This improvement was evident in fish administrated with the high CSNPs dose (0.66 ml/L) compared to catfish exposed to BPA in group (III). Furthermore, the percentage of hepatic DNA damage was detected in group III exposed to BPA alone. However, it was declined after co- administration with both the low and high doses of CSNPs. The study has revealed that treatment with CSNPs has antagonistic functions against the toxicity of BPA in female African catfish.

Authors+Show Affiliations

Department of Zoology, Faculty of women for Arts, Science & Education, Ain Shams University, Cairo 11757, Egypt. Electronic address: hebasalah84@women.asu.edu.eg.Department of Zoology, Faculty of women for Arts, Science & Education, Ain Shams University, Cairo 11757, Egypt.Department of Aquatic animals Diseases and Management, Faculty of Veterinary Medicine, Banha University, Banha, Egypt.Department of Zoology, Faculty of women for Arts, Science & Education, Ain Shams University, Cairo 11757, Egypt.

Pub Type(s)

Clinical Trial, Veterinary
Journal Article

Language

eng

PubMed ID

34146699

Citation

Hamed, Heba S., et al. "Chitosan Nanoparticles Alleviated Endocrine Disruption, Oxidative Damage, and Genotoxicity of Bisphenol-A- Intoxicated Female African Catfish." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 248, 2021, p. 109104.
Hamed HS, Ali RM, Shaheen AA, et al. Chitosan nanoparticles alleviated endocrine disruption, oxidative damage, and genotoxicity of Bisphenol-A- intoxicated female African catfish. Comp Biochem Physiol C Toxicol Pharmacol. 2021;248:109104.
Hamed, H. S., Ali, R. M., Shaheen, A. A., & Hussein, N. M. (2021). Chitosan nanoparticles alleviated endocrine disruption, oxidative damage, and genotoxicity of Bisphenol-A- intoxicated female African catfish. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 248, 109104. https://doi.org/10.1016/j.cbpc.2021.109104
Hamed HS, et al. Chitosan Nanoparticles Alleviated Endocrine Disruption, Oxidative Damage, and Genotoxicity of Bisphenol-A- Intoxicated Female African Catfish. Comp Biochem Physiol C Toxicol Pharmacol. 2021;248:109104. PubMed PMID: 34146699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chitosan nanoparticles alleviated endocrine disruption, oxidative damage, and genotoxicity of Bisphenol-A- intoxicated female African catfish. AU - Hamed,Heba S, AU - Ali,Rokaya M, AU - Shaheen,Adel A, AU - Hussein,Naema M, Y1 - 2021/06/17/ PY - 2021/04/10/received PY - 2021/05/31/revised PY - 2021/06/06/accepted PY - 2021/6/20/pubmed PY - 2021/12/24/medline PY - 2021/6/19/entrez KW - Bisphenol–A KW - Chitosan nanoparticles KW - Clarias gariepinus KW - DNA damage KW - Oxidative stress KW - Reproductive hormones SP - 109104 EP - 109104 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 248 N2 - Bisphenol-A (BPA) is widely used in production of plastic products. It can reach the ecosystems affecting aquatic organisms most likely fishes. The purpose of this study was to study the toxic effects of BPA on the biochemical variables and oxidative stress in female African catfish, Clarias gariepinus and to estimate the protective role of chitosan nanoparticles (CSNPs) against BPA toxicity. Five groups in triplicates of fish were divided as follows: group I was control, group II was treated with CSNPs (0.66 ml/L), group III was exposed to BPA (1.43 μg/L), group IV was treated with BPA (1.43 μg/L) plus CSNPs (0.33 ml/L), and group V was treated with BPA (1.43 μg/L) plus CSNPs (0.66 ml/L) for 30 days. Blood and liver tissue samples were collected at the end of experiment for the biochemical and oxidative stress biomarkers analyses. Results exhibited that serum Follicle Stimulating Hormone (FSH) and 17-β Estradiol (E2) were significantly decreased in female catfish. While, serum Testosterone (T.) and Luteinizing Hormone (LH) were increased after exposure to BPA. Marked increment in superoxide dismutase (SOD) and malondialdehyde (MDA) levels of hepatic tissue of catfish exposed to BPA. Furthermore, significant reduction in hepatic catalase (CAT), glutathione peroxidase (GSH-px), total antioxidant capacity (TAC), reduced glutathione (GSH), and glutathione S-transferase (GST) levels were decreased significantly in BPA-exposed catfish compared to the control group. However, administration of female C. gariepinus with the low and high doses (0.33 ml/L and 0.66 ml/L) of CNPs restored the biochemical parameters to be close to the normal values of the control group and also, reduced oxidative stress induced by BPA toxicity. This improvement was evident in fish administrated with the high CSNPs dose (0.66 ml/L) compared to catfish exposed to BPA in group (III). Furthermore, the percentage of hepatic DNA damage was detected in group III exposed to BPA alone. However, it was declined after co- administration with both the low and high doses of CSNPs. The study has revealed that treatment with CSNPs has antagonistic functions against the toxicity of BPA in female African catfish. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/34146699/Chitosan_nanoparticles_alleviated_endocrine_disruption_oxidative_damage_and_genotoxicity_of_Bisphenol_A__intoxicated_female_African_catfish_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(21)00131-9 DB - PRIME DP - Unbound Medicine ER -