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Anti-P 200 pemphigoid - The most common floor binding subepidermal autoimmune bullous disease in a tertiary care center in south India.
Indian J Dermatol Venereol Leprol. 2021 Nov-Dec; 87(6):787-791.IJ

Abstract

BACKGROUND

The pemphigoid group of diseases may present clinically and immunologically in a very similar fashion. Indirect immunofluorescence microscopy with readily available salt-split human skin in a BIOCHIP™ helps to classify these conditions as those with either with roof binding or floor binding of immunoreactants. Epidermolysis bullosa acquisita, anti-laminin 332 pemphigoid and anti-p200 pemphigoid show floor binding, while in the most frequent type of pemphigoid disease, bullous pemphigoid, epidermal side staining pattern is seen on salt-split skin Aims: The aim of the study was to detect the target antigens in sub-epidermal bullous diseases.

METHODS

Forty patients with bullous pemphigoid diagnosed by lesional histopathology and direct immunofluorescence microscopy were re-evaluated by a BIOCHIP™ mosaic containing both tissue substrates and recombinant target antigens. Sera with floor pattern staining on salt-split skin were further evaluated by immunoblotting with dermal extract.

RESULTS

Five patients with floor staining had anti-p200 pemphigoid.

LIMITATIONS

We could not perform serration pattern analysis of direct immunofluorescence in our patients.

CONCLUSION

Histopathology and direct immunofluorescence microscopy cannot differentiate between various entities of pemphigoid diseases. A multivariant approach using a BIOCHIP™ mosaic including salt-split skin followed by immunoblotting with dermal extract helps to identify the target antigen.

Authors+Show Affiliations

Department of Dermatology, PSG IMSR, Coimbatore, Tamil Nadu, India.Department of Dermatology, PSG IMSR, Coimbatore, Tamil Nadu, India.Department of Dermatology, PSG IMSR, Coimbatore, Tamil Nadu, India.Department of Dermatology, PSG IMSR, Coimbatore, Tamil Nadu, India.Department of Pathology, PSG IMSR, Coimbatore, Tamil Nadu, India.Department of Dermatology, University of Lübeck, Lübeck, Germany.Department of Dermatology, University of Lübeck, Lübeck, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34160166

Citation

Rai, Reena, et al. "Anti-P 200 Pemphigoid - the Most Common Floor Binding Subepidermal Autoimmune Bullous Disease in a Tertiary Care Center in South India." Indian Journal of Dermatology, Venereology and Leprology, vol. 87, no. 6, 2021, pp. 787-791.
Rai R, Anand JB, Shanmugasekar C, et al. Anti-P 200 pemphigoid - The most common floor binding subepidermal autoimmune bullous disease in a tertiary care center in south India. Indian J Dermatol Venereol Leprol. 2021;87(6):787-791.
Rai, R., Anand, J. B., Shanmugasekar, C., Arunprasath, P., Chaitra, V., Zillikens, D., & Schimdt, E. (2021). Anti-P 200 pemphigoid - The most common floor binding subepidermal autoimmune bullous disease in a tertiary care center in south India. Indian Journal of Dermatology, Venereology and Leprology, 87(6), 787-791. https://doi.org/10.25259/IJDVL_79_20
Rai R, et al. Anti-P 200 Pemphigoid - the Most Common Floor Binding Subepidermal Autoimmune Bullous Disease in a Tertiary Care Center in South India. Indian J Dermatol Venereol Leprol. 2021 Nov-Dec;87(6):787-791. PubMed PMID: 34160166.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-P 200 pemphigoid - The most common floor binding subepidermal autoimmune bullous disease in a tertiary care center in south India. AU - Rai,Reena, AU - Anand,J Bede, AU - Shanmugasekar,C, AU - Arunprasath,P, AU - Chaitra,V, AU - Zillikens,Detlef, AU - Schimdt,Enno, PY - 2020/01/01/received PY - 2021/01/01/accepted PY - 2021/6/24/pubmed PY - 2022/2/3/medline PY - 2021/6/23/entrez KW - Anti-p200 pemphigoid KW - biochip KW - floor binding KW - immunoblotting SP - 787 EP - 791 JF - Indian journal of dermatology, venereology and leprology JO - Indian J Dermatol Venereol Leprol VL - 87 IS - 6 N2 - BACKGROUND: The pemphigoid group of diseases may present clinically and immunologically in a very similar fashion. Indirect immunofluorescence microscopy with readily available salt-split human skin in a BIOCHIP™ helps to classify these conditions as those with either with roof binding or floor binding of immunoreactants. Epidermolysis bullosa acquisita, anti-laminin 332 pemphigoid and anti-p200 pemphigoid show floor binding, while in the most frequent type of pemphigoid disease, bullous pemphigoid, epidermal side staining pattern is seen on salt-split skin Aims: The aim of the study was to detect the target antigens in sub-epidermal bullous diseases. METHODS: Forty patients with bullous pemphigoid diagnosed by lesional histopathology and direct immunofluorescence microscopy were re-evaluated by a BIOCHIP™ mosaic containing both tissue substrates and recombinant target antigens. Sera with floor pattern staining on salt-split skin were further evaluated by immunoblotting with dermal extract. RESULTS: Five patients with floor staining had anti-p200 pemphigoid. LIMITATIONS: We could not perform serration pattern analysis of direct immunofluorescence in our patients. CONCLUSION: Histopathology and direct immunofluorescence microscopy cannot differentiate between various entities of pemphigoid diseases. A multivariant approach using a BIOCHIP™ mosaic including salt-split skin followed by immunoblotting with dermal extract helps to identify the target antigen. SN - 0973-3922 UR - https://www.unboundmedicine.com/medline/citation/34160166/Anti_P_200_pemphigoid___The_most_common_floor_binding_subepidermal_autoimmune_bullous_disease_in_a_tertiary_care_center_in_south_India_ L2 - https://doi.org/10.25259/IJDVL_79_20 DB - PRIME DP - Unbound Medicine ER -