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Detection of Serum Cross-Reactive Antibodies and Memory Response to SARS-CoV-2 in Prepandemic and Post-COVID-19 Convalescent Samples.
J Infect Dis. 2021 10 28; 224(8):1305-1315.JI

Abstract

BACKGROUND

A notable feature of coronavirus disease 2019 (COVID-19) is that children are less susceptible to severe disease. Children are known to experience more infections with endemic human coronaviruses (HCoVs) compared to adults. Little is known whether HCoV infections lead to cross-reactive anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies.

METHODS

We investigated the presence of cross-reactive anti-SARS-CoV-2 IgG antibodies to spike 1 (S1), S1-receptor-binding domain (S1-RBD), and nucleocapsid protein (NP) by enzyme-linked immunosorbent assays, and neutralizing activity by a SARS-CoV-2 pseudotyped virus neutralization assay, in prepandemic sera collected from children (n = 50) and adults (n = 45), and compared with serum samples from convalescent COVID-19 patients (n = 16).

RESULTS

A significant proportion of children (up to 40%) had detectable cross-reactive antibodies to SARS-CoV-2 S1, S1-RBD, and NP antigens, and the anti-S1 and anti-S1-RBD antibody levels correlated with anti-HCoV-HKU1 and anti-HCoV-OC43 S1 antibody titers in prepandemic samples (P < .001). There were marked increases of anti-HCoV-HKU1 and - OC43 S1 (but not anti-NL63 and -229E S1-RBD) antibody titers in serum samples from convalescent COVID-19 patients (P < .001), indicating an activation of cross-reactive immunological memory to β-coronavirus spike.

CONCLUSIONS

We demonstrated cross-reactive anti-SARS-CoV-2 antibodies in prepandemic serum samples from children and young adults. Promoting this cross-reactive immunity and memory response derived from common HCoV may be an effective strategy against SARS-COV-2 and future novel coronaviruses.

Authors+Show Affiliations

Department of Clinical Infection, Microbiology, and Immunology, Institute of Infection, Veterinary, and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.Ear Nose and Throat Department, Alder Hey Children's Hospital, Liverpool, United Kingdom.Ear Nose and Throat Department, Alder Hey Children's Hospital, Liverpool, United Kingdom.Liverpool Head and Neck Centre, University of Liverpool, Liverpool, United Kingdom.Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent, Chatham, United Kingdom.Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent, Chatham, United Kingdom.Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent, Chatham, United Kingdom.Department of Clinical Infection, Microbiology, and Immunology, Institute of Infection, Veterinary, and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.Department of Clinical Infection, Microbiology, and Immunology, Institute of Infection, Veterinary, and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.Institute of Child Health, Alder Hey Children's Hospital, Liverpool, United Kingdom.Department of Clinical Infection, Microbiology, and Immunology, Institute of Infection, Veterinary, and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.Department of Clinical Infection, Microbiology, and Immunology, Institute of Infection, Veterinary, and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.Department of Clinical Infection, Microbiology, and Immunology, Institute of Infection, Veterinary, and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34161567

Citation

Shrwani, Khalid, et al. "Detection of Serum Cross-Reactive Antibodies and Memory Response to SARS-CoV-2 in Prepandemic and Post-COVID-19 Convalescent Samples." The Journal of Infectious Diseases, vol. 224, no. 8, 2021, pp. 1305-1315.
Shrwani K, Sharma R, Krishnan M, et al. Detection of Serum Cross-Reactive Antibodies and Memory Response to SARS-CoV-2 in Prepandemic and Post-COVID-19 Convalescent Samples. J Infect Dis. 2021;224(8):1305-1315.
Shrwani, K., Sharma, R., Krishnan, M., Jones, T., Mayora-Neto, M., Cantoni, D., Temperton, N. J., Dobson, S. L., Subramaniam, K., McNamara, P. S., Cunliffe, N. A., Turtle, L., & Zhang, Q. (2021). Detection of Serum Cross-Reactive Antibodies and Memory Response to SARS-CoV-2 in Prepandemic and Post-COVID-19 Convalescent Samples. The Journal of Infectious Diseases, 224(8), 1305-1315. https://doi.org/10.1093/infdis/jiab333
Shrwani K, et al. Detection of Serum Cross-Reactive Antibodies and Memory Response to SARS-CoV-2 in Prepandemic and Post-COVID-19 Convalescent Samples. J Infect Dis. 2021 10 28;224(8):1305-1315. PubMed PMID: 34161567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Serum Cross-Reactive Antibodies and Memory Response to SARS-CoV-2 in Prepandemic and Post-COVID-19 Convalescent Samples. AU - Shrwani,Khalid, AU - Sharma,Ravi, AU - Krishnan,Madhan, AU - Jones,Terry, AU - Mayora-Neto,Martin, AU - Cantoni,Diego, AU - Temperton,Nigel J, AU - Dobson,Susan L, AU - Subramaniam,Krishanthi, AU - McNamara,Paul S, AU - Cunliffe,Nigel A, AU - Turtle,Lance, AU - Zhang,Qibo, PY - 2021/03/05/received PY - 2021/06/22/accepted PY - 2021/6/24/pubmed PY - 2021/11/9/medline PY - 2021/6/23/entrez KW - 19 KW - 2 KW - COVID KW - CoV KW - HCoV KW - NL63 KW - SARS KW - common human coronavirus (HCoV)-HKU1 KW - cross KW - immunological memory KW - reactive immunity KW - serum antibody SP - 1305 EP - 1315 JF - The Journal of infectious diseases JO - J Infect Dis VL - 224 IS - 8 N2 - BACKGROUND: A notable feature of coronavirus disease 2019 (COVID-19) is that children are less susceptible to severe disease. Children are known to experience more infections with endemic human coronaviruses (HCoVs) compared to adults. Little is known whether HCoV infections lead to cross-reactive anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies. METHODS: We investigated the presence of cross-reactive anti-SARS-CoV-2 IgG antibodies to spike 1 (S1), S1-receptor-binding domain (S1-RBD), and nucleocapsid protein (NP) by enzyme-linked immunosorbent assays, and neutralizing activity by a SARS-CoV-2 pseudotyped virus neutralization assay, in prepandemic sera collected from children (n = 50) and adults (n = 45), and compared with serum samples from convalescent COVID-19 patients (n = 16). RESULTS: A significant proportion of children (up to 40%) had detectable cross-reactive antibodies to SARS-CoV-2 S1, S1-RBD, and NP antigens, and the anti-S1 and anti-S1-RBD antibody levels correlated with anti-HCoV-HKU1 and anti-HCoV-OC43 S1 antibody titers in prepandemic samples (P < .001). There were marked increases of anti-HCoV-HKU1 and - OC43 S1 (but not anti-NL63 and -229E S1-RBD) antibody titers in serum samples from convalescent COVID-19 patients (P < .001), indicating an activation of cross-reactive immunological memory to β-coronavirus spike. CONCLUSIONS: We demonstrated cross-reactive anti-SARS-CoV-2 antibodies in prepandemic serum samples from children and young adults. Promoting this cross-reactive immunity and memory response derived from common HCoV may be an effective strategy against SARS-COV-2 and future novel coronaviruses. SN - 1537-6613 UR - https://www.unboundmedicine.com/medline/citation/34161567/Detection_of_Serum_Cross_Reactive_Antibodies_and_Memory_Response_to_SARS_CoV_2_in_Prepandemic_and_Post_COVID_19_Convalescent_Samples_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/jiab333 DB - PRIME DP - Unbound Medicine ER -