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Development and Molecular Cytogenetic Identification of a New Wheat-Psathyrostachys huashanica Keng Translocation Line Resistant to Powdery Mildew.
Front Plant Sci. 2021; 12:689502.FP

Abstract

Psathyrostachys huashanica Keng, a wild relative of common wheat with many desirable traits, is an invaluable source of genetic material for wheat improvement. Few wheat-P. huashanica translocation lines resistant to powdery mildew have been reported. In this study, a wheat-P. huashanica line, E24-3-1-6-2-1, was generated via distant hybridization, ethyl methanesulfonate (EMS) mutagenesis, and backcross breeding. A chromosome karyotype of 2n = 44 was observed at the mitotic stage in E24-3-1-6-2-1. Genomic in situ hybridization (GISH) analysis revealed four translocated chromosomes in E24-3-1-6-2-1, and P. huashanica chromosome-specific marker analysis showed that the alien chromosome fragment was from the P. huashanica 4Ns chromosome. Moreover, fluorescence in situ hybridization (FISH) analysis demonstrated that reciprocal translocation had occurred between the P. huashanica 4Ns chromosome and the wheat 3D chromosome; thus, E24-3-1-6-2-1 carried two translocations: T3DS·3DL-4NsL and T3DL-4NsS. Translocation also occurred between wheat chromosomes 2A and 4A. At the adult stage, E24-3-1-6-2-1 was highly resistant to powdery mildew, caused by prevalent pathotypes in China. Further, the spike length, numbers of fertile spikelets, kernels per spike, thousand-kernel weight, and grain yield of E24-3-1-6-2-1 were significantly higher than those of its wheat parent 7182 and addition line 24-6-3-1. Thus, this translocation line that is highly resistant to powdery mildew and has excellent agronomic traits can be used as a novel promising germplasm for breeding resistant and high-yielding cultivars.

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Authors+Show Affiliations

College of Agronomy, Northwest A&F University, Yangling, China.

College of Horticulture, Northwest A&F University, Yangling, China.

College of Agronomy, Northwest A&F University, Yangling, China.

College of Agronomy, Northwest A&F University, Yangling, China.

College of Agronomy, Northwest A&F University, Yangling, China.

College of Agronomy, Northwest A&F University, Yangling, China.

College of Agronomy, Northwest A&F University, Yangling, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34163516

Citation

Liu, Yuxiu, et al. "Development and Molecular Cytogenetic Identification of a New Wheat-Psathyrostachys Huashanica Keng Translocation Line Resistant to Powdery Mildew." Frontiers in Plant Science, vol. 12, 2021, p. 689502.

Liu Y, Huang S, Han J, et al. Development and Molecular Cytogenetic Identification of a New Wheat-Psathyrostachys huashanica Keng Translocation Line Resistant to Powdery Mildew. Front Plant Sci. 2021;12:689502.

Liu, Y., Huang, S., Han, J., Hou, C., Zheng, D., Zhang, Z., & Wu, J. (2021). Development and Molecular Cytogenetic Identification of a New Wheat-Psathyrostachys huashanica Keng Translocation Line Resistant to Powdery Mildew. Frontiers in Plant Science, 12, 689502. https://doi.org/10.3389/fpls.2021.689502

Liu Y, et al. Development and Molecular Cytogenetic Identification of a New Wheat-Psathyrostachys Huashanica Keng Translocation Line Resistant to Powdery Mildew. Front Plant Sci. 2021;12:689502. PubMed PMID: 34163516.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Development and Molecular Cytogenetic Identification of a New Wheat-Psathyrostachys huashanica Keng Translocation Line Resistant to Powdery Mildew. AU - Liu,Yuxiu, AU - Huang,Shuhua, AU - Han,Jing, AU - Hou,Chenchen, AU - Zheng,Dasheng, AU - Zhang,Zhengmao, AU - Wu,Jun, Y1 - 2021/06/07/ PY - 2021/04/01/received PY - 2021/05/18/accepted PY - 2021/6/24/entrez PY - 2021/6/25/pubmed PY - 2021/6/25/medline KW - P. huashanica KW - agronomic performance KW - translocation line KW - wheat KW - wheat powdery mildew SP - 689502 EP - 689502 JF - Frontiers in plant science JO - Front Plant Sci VL - 12 N2 - Psathyrostachys huashanica Keng, a wild relative of common wheat with many desirable traits, is an invaluable source of genetic material for wheat improvement. Few wheat-P. huashanica translocation lines resistant to powdery mildew have been reported. In this study, a wheat-P. huashanica line, E24-3-1-6-2-1, was generated via distant hybridization, ethyl methanesulfonate (EMS) mutagenesis, and backcross breeding. A chromosome karyotype of 2n = 44 was observed at the mitotic stage in E24-3-1-6-2-1. Genomic in situ hybridization (GISH) analysis revealed four translocated chromosomes in E24-3-1-6-2-1, and P. huashanica chromosome-specific marker analysis showed that the alien chromosome fragment was from the P. huashanica 4Ns chromosome. Moreover, fluorescence in situ hybridization (FISH) analysis demonstrated that reciprocal translocation had occurred between the P. huashanica 4Ns chromosome and the wheat 3D chromosome; thus, E24-3-1-6-2-1 carried two translocations: T3DS·3DL-4NsL and T3DL-4NsS. Translocation also occurred between wheat chromosomes 2A and 4A. At the adult stage, E24-3-1-6-2-1 was highly resistant to powdery mildew, caused by prevalent pathotypes in China. Further, the spike length, numbers of fertile spikelets, kernels per spike, thousand-kernel weight, and grain yield of E24-3-1-6-2-1 were significantly higher than those of its wheat parent 7182 and addition line 24-6-3-1. Thus, this translocation line that is highly resistant to powdery mildew and has excellent agronomic traits can be used as a novel promising germplasm for breeding resistant and high-yielding cultivars. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/34163516/Development_and_Molecular_Cytogenetic_Identification_of_a_New_Wheat_Psathyrostachys_huashanica_Keng_Translocation_Line_Resistant_to_Powdery_Mildew_ DB - PRIME DP - Unbound Medicine ER -