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Role of dorsal hippocampal muscarinic receptor activity in acquisition and retention of single- versus multiple-trial contextual fear conditioning in adolescent rats.
Behav Neurosci. 2021 Aug; 135(4):540-549.BN

Abstract

The present study examined the effects of the muscarinic acetylcholine receptor (mAChR) antagonist, scopolamine, on standard contextual fear conditioning (sCFC). It compared effects of the drug on acquisition (post-shock freezing) versus 24-hr retention of a context-shock association acquired after one or three pairings of a context with unsignaled shock. During single-trial sCFC, systemic scopolamine (0.5 mg/kg, i.p.) prior to training abolished both post-shock and retention freezing (Experiment 1). This same injection during multiple-trial sCFC also abolished post-shock freezing and impaired 24-hr retention freezing (Experiment 2). These results indicate that cholinergic signaling mediates both acquisition and 24-hr retention of a context-shock association across different trial parameters. Experiment 3 further explored these effects by infusing scopolamine (35 μg per side) into the dorsal hippocampus (dHPC) prior to training in single versus multiple-trial sCFC. This infusion spared post-shock but abolished retention test freezing in single-trial sCFC (Experiment 3A), and had no effect on multiple-trial sCFC (Experiment 3B). The current findings suggest that brain-wide cholinergic signaling mediates acquisition and retention of single-trial sCFC. Despite this, while muscarinic cholinergic signaling in the dHPC does mediate retention of single-trial sCFC, it is not required for acquisition of either variant, or retention of multiple-trial sCFC. These findings also rule out impaired sensory processing of contextual cues as a mechanism of impaired context learning by dHPC scopolamine. The results are discussed in relation to the role of cholinergic function across multiple brain memory systems in elemental versus configural forms of contextual fear conditioning (Fanselow, 2010; Rudy, 2009). (PsycInfo Database Record (c) 2021 APA, all rights reserved).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Pinizzotto CC
Department of Psychological and Brain Sciences, University of Delaware.
Partie ME
Department of Psychological and Brain Sciences, University of Delaware.
Heroux NA
Department of Psychological and Brain Sciences, University of Delaware.
Stanton ME
Department of Psychological and Brain Sciences, University of Delaware.

MeSH

AnimalsConditioning, ClassicalFearFreezing Reaction, CatalepticHippocampusRatsRats, Long-EvansReceptors, Muscarinic

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34165996

Citation

Pinizzotto, Claudia C., et al. "Role of Dorsal Hippocampal Muscarinic Receptor Activity in Acquisition and Retention of Single- Versus Multiple-trial Contextual Fear Conditioning in Adolescent Rats." Behavioral Neuroscience, vol. 135, no. 4, 2021, pp. 540-549.
Pinizzotto CC, Partie ME, Heroux NA, et al. Role of dorsal hippocampal muscarinic receptor activity in acquisition and retention of single- versus multiple-trial contextual fear conditioning in adolescent rats. Behav Neurosci. 2021;135(4):540-549.
Pinizzotto, C. C., Partie, M. E., Heroux, N. A., & Stanton, M. E. (2021). Role of dorsal hippocampal muscarinic receptor activity in acquisition and retention of single- versus multiple-trial contextual fear conditioning in adolescent rats. Behavioral Neuroscience, 135(4), 540-549. https://doi.org/10.1037/bne0000469
Pinizzotto CC, et al. Role of Dorsal Hippocampal Muscarinic Receptor Activity in Acquisition and Retention of Single- Versus Multiple-trial Contextual Fear Conditioning in Adolescent Rats. Behav Neurosci. 2021;135(4):540-549. PubMed PMID: 34165996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of dorsal hippocampal muscarinic receptor activity in acquisition and retention of single- versus multiple-trial contextual fear conditioning in adolescent rats. AU - Pinizzotto,Claudia C, AU - Partie,Miranda E, AU - Heroux,Nicholas A, AU - Stanton,Mark E, Y1 - 2021/06/24/ PY - 2021/6/25/pubmed PY - 2021/9/18/medline PY - 2021/6/24/entrez SP - 540 EP - 549 JF - Behavioral neuroscience JO - Behav Neurosci VL - 135 IS - 4 N2 - The present study examined the effects of the muscarinic acetylcholine receptor (mAChR) antagonist, scopolamine, on standard contextual fear conditioning (sCFC). It compared effects of the drug on acquisition (post-shock freezing) versus 24-hr retention of a context-shock association acquired after one or three pairings of a context with unsignaled shock. During single-trial sCFC, systemic scopolamine (0.5 mg/kg, i.p.) prior to training abolished both post-shock and retention freezing (Experiment 1). This same injection during multiple-trial sCFC also abolished post-shock freezing and impaired 24-hr retention freezing (Experiment 2). These results indicate that cholinergic signaling mediates both acquisition and 24-hr retention of a context-shock association across different trial parameters. Experiment 3 further explored these effects by infusing scopolamine (35 μg per side) into the dorsal hippocampus (dHPC) prior to training in single versus multiple-trial sCFC. This infusion spared post-shock but abolished retention test freezing in single-trial sCFC (Experiment 3A), and had no effect on multiple-trial sCFC (Experiment 3B). The current findings suggest that brain-wide cholinergic signaling mediates acquisition and retention of single-trial sCFC. Despite this, while muscarinic cholinergic signaling in the dHPC does mediate retention of single-trial sCFC, it is not required for acquisition of either variant, or retention of multiple-trial sCFC. These findings also rule out impaired sensory processing of contextual cues as a mechanism of impaired context learning by dHPC scopolamine. The results are discussed in relation to the role of cholinergic function across multiple brain memory systems in elemental versus configural forms of contextual fear conditioning (Fanselow, 2010; Rudy, 2009). (PsycInfo Database Record (c) 2021 APA, all rights reserved). SN - 1939-0084 UR - https://www.unboundmedicine.com/medline/citation/34165996/Role_of_dorsal_hippocampal_muscarinic_receptor_activity_in_acquisition_and_retention_of_single__versus_multiple_trial_contextual_fear_conditioning_in_adolescent_rats_ DB - PRIME DP - Unbound Medicine ER -