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Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae.
BMC Microbiol. 2021 06 29; 21(1):198.BM

Abstract

BACKGROUND

Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum, isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae. Strains were evaluated for their physiological, safety, and antimicrobial characteristics.

RESULTS

The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae. All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated.

CONCLUSION

The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections.

Authors+Show Affiliations

Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900, Ilhéus, BA, Brazil.Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900, Ilhéus, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Professor Lineu Prestes, 2415, CEP 05508-900, São Paulo, SP, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900, Ilhéus, BA, Brazil.Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900, Ilhéus, BA, Brazil.Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900, Ilhéus, BA, Brazil. Departamento de Ciências Biológicas, Laboratório de Microbiologia da Agroindústria, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900, Ilhéus, BA, Brazil.Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil.Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900, Ilhéus, BA, Brazil. Departamento de Ciências Biológicas, Laboratório de Microbiologia da Agroindústria, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900, Ilhéus, BA, Brazil.Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Salobrinho, Rodovia Jorge Amado, Km 16, CEP 45662-900, Ilhéus, BA, Brazil. lmirandamarques@gmail.com. Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Campus Anísio Teixeira, Rua Hormindo Barros, 58, CEP 45029-094, Vitória da Conquista, BA, Brazil. lmirandamarques@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34187371

Citation

das Neves Selis, Nathan, et al. "Lactiplantibacillus Plantarum Strains Isolated From Spontaneously Fermented Cocoa Exhibit Potential Probiotic Properties Against Gardnerella Vaginalis and Neisseria Gonorrhoeae." BMC Microbiology, vol. 21, no. 1, 2021, p. 198.
das Neves Selis N, de Oliveira HBM, Leão HF, et al. Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae. BMC Microbiol. 2021;21(1):198.
das Neves Selis, N., de Oliveira, H. B. M., Leão, H. F., Dos Anjos, Y. B., Sampaio, B. A., Correia, T. M. L., Almeida, C. F., Pena, L. S. C., Reis, M. M., Brito, T. L. S., Brito, L. F., Campos, G. B., Timenetsky, J., Cruz, M. P., Rezende, R. P., Romano, C. C., da Costa, A. M., Yatsuda, R., Uetanabaro, A. P. T., & Marques, L. M. (2021). Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae. BMC Microbiology, 21(1), 198. https://doi.org/10.1186/s12866-021-02264-5
das Neves Selis N, et al. Lactiplantibacillus Plantarum Strains Isolated From Spontaneously Fermented Cocoa Exhibit Potential Probiotic Properties Against Gardnerella Vaginalis and Neisseria Gonorrhoeae. BMC Microbiol. 2021 06 29;21(1):198. PubMed PMID: 34187371.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae. AU - das Neves Selis,Nathan, AU - de Oliveira,Hellen Braga Martins, AU - Leão,Hiago Ferreira, AU - Dos Anjos,Yan Bento, AU - Sampaio,Beatriz Almeida, AU - Correia,Thiago Macêdo Lopes, AU - Almeida,Carolline Florentino, AU - Pena,Larissa Silva Carvalho, AU - Reis,Mariane Mares, AU - Brito,Thamara Louisy Santos, AU - Brito,Laís Ferraz, AU - Campos,Guilherme Barreto, AU - Timenetsky,Jorge, AU - Cruz,Mariluze Peixoto, AU - Rezende,Rachel Passos, AU - Romano,Carla Cristina, AU - da Costa,Andréa Miura, AU - Yatsuda,Regiane, AU - Uetanabaro,Ana Paula Trovatti, AU - Marques,Lucas Miranda, Y1 - 2021/06/29/ PY - 2021/02/25/received PY - 2021/06/15/accepted PY - 2021/6/30/entrez PY - 2021/7/1/pubmed PY - 2021/7/1/medline KW - Cell-free culture supernatant KW - G. vaginalis KW - L. plantarum KW - Metabolome KW - N. gonorrhoeae KW - Organic acids KW - Probiotics SP - 198 EP - 198 JF - BMC microbiology JO - BMC Microbiol VL - 21 IS - 1 N2 - BACKGROUND: Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum, isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae. Strains were evaluated for their physiological, safety, and antimicrobial characteristics. RESULTS: The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae. All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated. CONCLUSION: The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/34187371/Lactiplantibacillus_plantarum_strains_isolated_from_spontaneously_fermented_cocoa_exhibit_potential_probiotic_properties_against_Gardnerella_vaginalis_and_Neisseria_gonorrhoeae_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-021-02264-5 DB - PRIME DP - Unbound Medicine ER -