TY - JOUR T1 - Molecular Detection and Genetic Characterization of Orientia tsutsugamushi from Hospitalized Acute Encephalitis Syndrome Cases During Two Consecutive Outbreaks in Eastern Uttar Pradesh, India. AU - Behera,Sthita Pragnya, AU - Kumar,Niraj, AU - Singh,Rajeev, AU - Deval,Hirawati, AU - Zaman,Kamran, AU - Misra,Brijranjan, AU - Pandey,Ashok, AU - Kant,Rajni, AU - Kavathekar,Asif, AU - Kumar,Sanjeev, AU - Nuthakki,Madhava Rao, AU - Bondre,Vijay P, Y1 - 2021/06/29/ PY - 2021/7/1/pubmed PY - 2022/1/27/medline PY - 2021/6/30/entrez KW - 56-kDa type-specific antigen gene KW - Orientia tsutsugamushi KW - acute encephalitis syndrome KW - phylogenetic analysis SP - 747 EP - 752 JF - Vector borne and zoonotic diseases (Larchmont, N.Y.) JO - Vector Borne Zoonotic Dis VL - 21 IS - 10 N2 - Background: Seasonal outbreaks of acute encephalitis syndrome (AES) have been reported especially in the pediatric population with a high case fatality rate in Eastern Uttar Pradesh, India. Orientia tsutsugamushi (OT) is a causative agent of scrub typhus that has been recently identified as a major cause of AES. However, the specific genotypes of OT responsible for AES cases of this region are not known. Therefore, the present study was undertaken to understand the molecular epidemiology of OT prevailing in the AES endemic Eastern Uttar Pradesh region of India. Methods: The study was conducted on 2529 hospitalized AES cases from August 2016 to December 2017. The presence of antibodies against OT from cerebrospinal fluid (CSF) and serum samples were tested using OT IgM enzyme-linked immunosorbent assay (ELISA), whereas OT DNA was tested from whole blood and CSF specimens targeting the partial gene of 56 kDa using nested PCR. Phylogenetic analysis was conducted with sequences (n = 241) generated in this study. Findings: Among the studied AES cases, 50% were found positive for antibodies against OT, whereas 37% of cases were positive for OT DNA. The genetic analysis study revealed that Gilliam (93.8%) is the prevailing genotype of OT followed by Karp (6.16%) genotype in AES cases. Furthermore, the Gilliam strains of this study showed they were >99% identical to earlier reported Gilliam strains from AES cases. Conclusion: We observed the presence of two main OT genotypes in AES cases, among which the majority of OT genotypes fall under the Gilliam clade. The understanding of predominant genotype will be beneficial for its future implications in vaccine development strategies and the development of rapid diagnostic tests. SN - 1557-7759 UR - https://www.unboundmedicine.com/medline/citation/34191633/Molecular_Detection_and_Genetic_Characterization_of_Orientia_tsutsugamushi_from_Hospitalized_Acute_Encephalitis_Syndrome_Cases_During_Two_Consecutive_Outbreaks_in_Eastern_Uttar_Pradesh_India_ DB - PRIME DP - Unbound Medicine ER -