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Açai (Euterpe oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line.
Molecules. 2021 Jun 10; 26(12)M

Abstract

Euterpe oleracea Mart. (açai) is a native palm from the Amazon region. There are various chemical constituents of açai with bioactive properties. This study aimed to evaluate the chemical composition and cytotoxic effects of açai seed extract on breast cancer cell line (MCF-7). Global Natural Products Social Molecular Networking (GNPS) was applied to identify chemical compounds present in açai seed extract. LC-MS/MS and molecular networking were employed to detect the phenolic compounds of açai. The antioxidant activity of açai seed extract was measured by DPPH assay. MCF-7 breast cancer cell line viability was evaluated by MTT assay. Cell death was evaluated by flow cytometry and time-lapse microscopy. Autophagy was evaluated by orange acridin immunofluorescence assay. Reactive oxygen species (ROS) production was evaluated by DAF assay. From the molecular networking, fifteen compounds were identified, mainly phenolic compounds. The açai seed extract showed cytotoxic effects against MCF-7, induced morphologic changes in the cell line by autophagy and increased the ROS production pathway. The present study suggests that açai seed extract has a high cytotoxic capacity and may induce autophagy by increasing ROS production in breast cancer. Apart from its antioxidant activity, flavonoids with high radical scavenging activity present in açai also generated NO (nitric oxide), contributing to its cytotoxic effect and autophagy induction.

Authors+Show Affiliations

Post-Graduate Program in Internal Medicine, Faculty of Medical Science, Universidade Estadual de Campinas, Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz. CEP, Campinas 13083-887, SP, Brazil.Institute of Chemistry, Universidade Estadual de Campinas, CP 6154, Campinas 13083-970, SP, Brazil.Institute of Chemistry, Universidade Estadual de Campinas, CP 6154, Campinas 13083-970, SP, Brazil.Faculty of Pharmaceutical Sciences, Universidade Estadual de Campinas, Campinas 13083-859, SP, Brazil.Post-Graduate Program in Adult Heath, Department of Patology, Federal University of Maranhão (UFMA), São Luís 65080-805, MA, Brazil.Post-Graduate Program in Adult Heath, Department of Patology, Federal University of Maranhão (UFMA), São Luís 65080-805, MA, Brazil.Post-Graduate Program in Health Sicencies, Federal University of Maranhão (UFMA), São Luís 65080-805, MA, Brazil.Post-Graduate Program in Health Sicencies, Federal University of Maranhão (UFMA), São Luís 65080-805, MA, Brazil.Faculty of Pharmaceutical Sciences, Universidade Estadual de Campinas, Campinas 13083-859, SP, Brazil.Faculty of Pharmaceutical Sciences, Universidade Estadual de Campinas, Campinas 13083-859, SP, Brazil.Post-Graduate Program in Family Health, Department of Medicine I, Federal University of Maranhão (UFMA), São Luís 65080-805, MA, Brazil.Post-Graduate Program in Adult Heath, Department of Patology, Federal University of Maranhão (UFMA), São Luís 65080-805, MA, Brazil.Faculty of Pharmaceutical Sciences, Universidade Estadual de Campinas, Campinas 13083-859, SP, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34200718

Citation

Silva, Marcos Antonio Custódio Neto da, et al. "Açai (Euterpe Oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line." Molecules (Basel, Switzerland), vol. 26, no. 12, 2021.
Silva MACND, Costa JH, Pacheco-Fill T, et al. Açai (Euterpe oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line. Molecules. 2021;26(12).
Silva, M. A. C. N. D., Costa, J. H., Pacheco-Fill, T., Ruiz, A. L. T. G., Vidal, F. C. B., Borges, K. R. A., Guimarães, S. J. A., Azevedo-Santos, A. P. S., Buglio, K. E., Foglio, M. A., Barbosa, M. D. C. L., Nascimento, M. D. D. S. B., & de Carvalho, J. E. (2021). Açai (Euterpe oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line. Molecules (Basel, Switzerland), 26(12). https://doi.org/10.3390/molecules26123546
Silva MACND, et al. Açai (Euterpe Oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line. Molecules. 2021 Jun 10;26(12) PubMed PMID: 34200718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Açai (Euterpe oleracea Mart.) Seed Extract Induces ROS Production and Cell Death in MCF-7 Breast Cancer Cell Line. AU - Silva,Marcos Antonio Custódio Neto da, AU - Costa,Jonas Henrique, AU - Pacheco-Fill,Taícia, AU - Ruiz,Ana Lúcia Tasca Gois, AU - Vidal,Flávia Castello Branco, AU - Borges,Kátia Regina Assunção, AU - Guimarães,Sulayne Janaina Araújo, AU - Azevedo-Santos,Ana Paula Silva de, AU - Buglio,Kaio Eduardo, AU - Foglio,Mary Ann, AU - Barbosa,Maria do Carmo Lacerda, AU - Nascimento,Maria do Desterro Soares Brandão, AU - de Carvalho,João Ernesto, Y1 - 2021/06/10/ PY - 2021/05/11/received PY - 2021/06/06/revised PY - 2021/06/07/accepted PY - 2021/7/2/entrez PY - 2021/7/3/pubmed PY - 2021/7/14/medline KW - Euterpe oleracea Mart. KW - autophagy KW - breast cancer KW - flavonoids KW - mass spectrometry KW - reactive oxygen species JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 12 N2 - Euterpe oleracea Mart. (açai) is a native palm from the Amazon region. There are various chemical constituents of açai with bioactive properties. This study aimed to evaluate the chemical composition and cytotoxic effects of açai seed extract on breast cancer cell line (MCF-7). Global Natural Products Social Molecular Networking (GNPS) was applied to identify chemical compounds present in açai seed extract. LC-MS/MS and molecular networking were employed to detect the phenolic compounds of açai. The antioxidant activity of açai seed extract was measured by DPPH assay. MCF-7 breast cancer cell line viability was evaluated by MTT assay. Cell death was evaluated by flow cytometry and time-lapse microscopy. Autophagy was evaluated by orange acridin immunofluorescence assay. Reactive oxygen species (ROS) production was evaluated by DAF assay. From the molecular networking, fifteen compounds were identified, mainly phenolic compounds. The açai seed extract showed cytotoxic effects against MCF-7, induced morphologic changes in the cell line by autophagy and increased the ROS production pathway. The present study suggests that açai seed extract has a high cytotoxic capacity and may induce autophagy by increasing ROS production in breast cancer. Apart from its antioxidant activity, flavonoids with high radical scavenging activity present in açai also generated NO (nitric oxide), contributing to its cytotoxic effect and autophagy induction. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/34200718/Açai__Euterpe_oleracea_Mart___Seed_Extract_Induces_ROS_Production_and_Cell_Death_in_MCF_7_Breast_Cancer_Cell_Line_ L2 - https://www.mdpi.com/resolver?pii=molecules26123546 DB - PRIME DP - Unbound Medicine ER -