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Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles.
Nanomaterials (Basel). 2021 Jun 03; 11(6)N

Abstract

In this study, we aimed to develop a technique for colloidal silver nanoparticle (AgNP) modification in order to increase their stability in aqueous suspensions. For this purpose, 40-nm spherical AgNPs were modified by the addition of either human albumin or Tween-80 (Polysorbate-80). After detailed characterization of their physicochemical properties, the hemolytic activity of the nonmodified and modified AgNPs was investigated, as well as their cytotoxicity and antimicrobial effects. Both albumin- and Tween-80-coated AgNPs demonstrated excellent stability in 0.9% sodium chloride solution (>12 months) compared to nonmodified AgNPs, characterized by their rapid precipitation. Hemolytic activity of nonmodified and albumin-coated AgNPs was found to be minimal, while Tween-80-modified AgNPs produced significant hemolysis after 1, 2, and 24 h of incubation. In addition, both native and Tween-80-covered AgNPs showed dose-dependent cytotoxic effects on human adipose-tissue-derived mesenchymal stem cells. The albumin-coated AgNPs showed minimal cytotoxicity. The antimicrobial effects of native and albumin-coated AgNPs against S. aureus, K. pneumonia, P. aeruginosa, Corynebacterium spp., and Acinetobacter spp. were statistically significant. We conclude that albumin coating of AgNPs significantly contributes to improve stability, reduce cytotoxicity, and confers potent antimicrobial action.

Authors+Show Affiliations

Almazov National Research Centre, 197341 Saint-Petersburg, Russia. Pavlov First Saint-Petersburg State Medical University, 197022 Saint-Petersburg, Russia.Almazov National Research Centre, 197341 Saint-Petersburg, Russia. Saint-Petersburg Electrotechnical University "LETI-ETU" Named after V.I.Ulyanov (Lenin), 197376 Saint-Petersburg, Russia.Almazov National Research Centre, 197341 Saint-Petersburg, Russia.Almazov National Research Centre, 197341 Saint-Petersburg, Russia. Pavlov First Saint-Petersburg State Medical University, 197022 Saint-Petersburg, Russia.Almazov National Research Centre, 197341 Saint-Petersburg, Russia.Almazov National Research Centre, 197341 Saint-Petersburg, Russia.Saint-Petersburg Research Institute of Influenza, 197376 Saint-Petersburg, Russia.Pavlov First Saint-Petersburg State Medical University, 197022 Saint-Petersburg, Russia. Federal State Institution Saint-Petersburg Pasteur Research Institute of Epidemiology and Microbiology, 197101 Saint-Petersburg, Russia.Pavlov First Saint-Petersburg State Medical University, 197022 Saint-Petersburg, Russia.Saint-Petersburg Electrotechnical University "LETI-ETU" Named after V.I.Ulyanov (Lenin), 197376 Saint-Petersburg, Russia.Almazov National Research Centre, 197341 Saint-Petersburg, Russia. Pavlov First Saint-Petersburg State Medical University, 197022 Saint-Petersburg, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34205084

Citation

Korolev, Dmitry, et al. "Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles." Nanomaterials (Basel, Switzerland), vol. 11, no. 6, 2021.
Korolev D, Shumilo M, Shulmeyster G, et al. Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles. Nanomaterials (Basel). 2021;11(6).
Korolev, D., Shumilo, M., Shulmeyster, G., Krutikov, A., Golovkin, A., Mishanin, A., Gorshkov, A., Spiridonova, A., Domorad, A., Krasichkov, A., & Galagudza, M. (2021). Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles. Nanomaterials (Basel, Switzerland), 11(6). https://doi.org/10.3390/nano11061484
Korolev D, et al. Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles. Nanomaterials (Basel). 2021 Jun 3;11(6) PubMed PMID: 34205084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles. AU - Korolev,Dmitry, AU - Shumilo,Michael, AU - Shulmeyster,Galina, AU - Krutikov,Alexander, AU - Golovkin,Alexey, AU - Mishanin,Alexander, AU - Gorshkov,Andrew, AU - Spiridonova,Anna, AU - Domorad,Anna, AU - Krasichkov,Alexander, AU - Galagudza,Michael, Y1 - 2021/06/03/ PY - 2021/04/30/received PY - 2021/05/28/revised PY - 2021/05/31/accepted PY - 2021/7/2/entrez PY - 2021/7/3/pubmed PY - 2021/7/3/medline KW - antimicrobial activity KW - chemical synthesis KW - cytotoxicity KW - hemolysis KW - silver nanoparticles JF - Nanomaterials (Basel, Switzerland) JO - Nanomaterials (Basel) VL - 11 IS - 6 N2 - In this study, we aimed to develop a technique for colloidal silver nanoparticle (AgNP) modification in order to increase their stability in aqueous suspensions. For this purpose, 40-nm spherical AgNPs were modified by the addition of either human albumin or Tween-80 (Polysorbate-80). After detailed characterization of their physicochemical properties, the hemolytic activity of the nonmodified and modified AgNPs was investigated, as well as their cytotoxicity and antimicrobial effects. Both albumin- and Tween-80-coated AgNPs demonstrated excellent stability in 0.9% sodium chloride solution (>12 months) compared to nonmodified AgNPs, characterized by their rapid precipitation. Hemolytic activity of nonmodified and albumin-coated AgNPs was found to be minimal, while Tween-80-modified AgNPs produced significant hemolysis after 1, 2, and 24 h of incubation. In addition, both native and Tween-80-covered AgNPs showed dose-dependent cytotoxic effects on human adipose-tissue-derived mesenchymal stem cells. The albumin-coated AgNPs showed minimal cytotoxicity. The antimicrobial effects of native and albumin-coated AgNPs against S. aureus, K. pneumonia, P. aeruginosa, Corynebacterium spp., and Acinetobacter spp. were statistically significant. We conclude that albumin coating of AgNPs significantly contributes to improve stability, reduce cytotoxicity, and confers potent antimicrobial action. SN - 2079-4991 UR - https://www.unboundmedicine.com/medline/citation/34205084/Hemolytic_Activity,_Cytotoxicity,_and_Antimicrobial_Effects_of_Human_Albumin-_and_Polysorbate-80-Coated_Silver_Nanoparticles. L2 - https://www.mdpi.com/resolver?pii=nano11061484 DB - PRIME DP - Unbound Medicine ER -
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