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DNA methylation and behavioral dysfunction in males with 47,XXY and 49,XXXXY: a pilot study.
Clin Epigenetics. 2021 07 01; 13(1):136.CE

Abstract

BACKGROUND

Equal dosage of X-linked genes between males and females is maintained by the X-inactivation of the second X chromosome in females through epigenetic mechanisms. Boys with aneuploidy of the X chromosome exhibit a host of symptoms such as low fertility, musculoskeletal anomalies, and cognitive and behavioral deficits that are presumed to be caused by the abnormal dosage of these genes. The objective of this pilot study is to assess the relationship between CpG methylation, an epigenetic modification, at several genes on the X chromosome and behavioral dysfunction in boys with supernumerary X chromosomes.

RESULTS

Two parental questionnaires, the Behavior Rating Inventory of Executive Function (BRIEF) and Child Behavior Checklist (CBCL), were analyzed, and they showed expected differences in both internal and external behaviors between neurotypical (46,XY) boys and boys with 49,XXXXY. There were several CpGs in AR and MAOA of boys with 49,XXXXY whose methylation levels were skewed from levels predicted from having one active (Xa) and three inactive (Xi) X chromosomes. Further, methylation levels of multiple CpGs in MAOA showed nominally significant association with externalizing behavior on the CBCL, and the methylation level of one CpG in AR showed nominally significant association with the BRIEF Regulation Index.

CONCLUSIONS

Boys with 49,XXXXY displayed higher levels of CpG methylation at regulatory intronic regions in X-linked genes encoding the androgen receptor (AR) and monoamine oxidase A (MAOA), compared to that in boys with 47,XXY and neurotypical boys. Our pilot study results suggest a link between CpG methylation levels and behavior in boys with 49,XXXXY.

Authors+Show Affiliations

The Mood Disorders Center, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.Department of Research, The Focus Foundation, Davidsonville, MD, USA.Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA.The Mood Disorders Center, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.Department of Research, The Focus Foundation, Davidsonville, MD, USA.Kennedy Krieger Institute, Baltimore, MD, USA.Kennedy Krieger Institute, Baltimore, MD, USA.Department of Neurology, George Washington University, Washington, DC, USA. Division of Neurogenetics and Developmental Pediatrics, Children's National Health System, Washington, DC, USA.Department of Research, The Focus Foundation, Davidsonville, MD, USA. cssprouse@email.gwu.edu. Department of Pediatrics, George Washington University, Washington, DC, USA. cssprouse@email.gwu.edu. Department of Human and Molecular Genetics, Florida International University, Miami, FL, USA. cssprouse@email.gwu.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34210361

Citation

Lee, Richard S., et al. "DNA Methylation and Behavioral Dysfunction in Males With 47,XXY and 49,XXXXY: a Pilot Study." Clinical Epigenetics, vol. 13, no. 1, 2021, p. 136.
Lee RS, Song SQ, Garrison-Desany HM, et al. DNA methylation and behavioral dysfunction in males with 47,XXY and 49,XXXXY: a pilot study. Clin Epigenetics. 2021;13(1):136.
Lee, R. S., Song, S. Q., Garrison-Desany, H. M., Carey, J. L., Lasutschinkow, P., Zabel, A., Bressler, J., Gropman, A., & Samango-Sprouse, C. (2021). DNA methylation and behavioral dysfunction in males with 47,XXY and 49,XXXXY: a pilot study. Clinical Epigenetics, 13(1), 136. https://doi.org/10.1186/s13148-021-01123-4
Lee RS, et al. DNA Methylation and Behavioral Dysfunction in Males With 47,XXY and 49,XXXXY: a Pilot Study. Clin Epigenetics. 2021 07 1;13(1):136. PubMed PMID: 34210361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA methylation and behavioral dysfunction in males with 47,XXY and 49,XXXXY: a pilot study. AU - Lee,Richard S, AU - Song,Sophia Q, AU - Garrison-Desany,Henri M, AU - Carey,Jenny L, AU - Lasutschinkow,Patricia, AU - Zabel,Andrew, AU - Bressler,Joseph, AU - Gropman,Andrea, AU - Samango-Sprouse,Carole, Y1 - 2021/07/01/ PY - 2021/04/01/received PY - 2021/06/27/accepted PY - 2021/7/2/entrez PY - 2021/7/3/pubmed PY - 2022/2/1/medline KW - 47,XXY KW - 49,XXXXY KW - DNA methylation KW - Executive functioning KW - Externalizing disorders KW - Internalizing disorders KW - MAOA KW - Sex chromosome aneuploidies SP - 136 EP - 136 JF - Clinical epigenetics JO - Clin Epigenetics VL - 13 IS - 1 N2 - BACKGROUND: Equal dosage of X-linked genes between males and females is maintained by the X-inactivation of the second X chromosome in females through epigenetic mechanisms. Boys with aneuploidy of the X chromosome exhibit a host of symptoms such as low fertility, musculoskeletal anomalies, and cognitive and behavioral deficits that are presumed to be caused by the abnormal dosage of these genes. The objective of this pilot study is to assess the relationship between CpG methylation, an epigenetic modification, at several genes on the X chromosome and behavioral dysfunction in boys with supernumerary X chromosomes. RESULTS: Two parental questionnaires, the Behavior Rating Inventory of Executive Function (BRIEF) and Child Behavior Checklist (CBCL), were analyzed, and they showed expected differences in both internal and external behaviors between neurotypical (46,XY) boys and boys with 49,XXXXY. There were several CpGs in AR and MAOA of boys with 49,XXXXY whose methylation levels were skewed from levels predicted from having one active (Xa) and three inactive (Xi) X chromosomes. Further, methylation levels of multiple CpGs in MAOA showed nominally significant association with externalizing behavior on the CBCL, and the methylation level of one CpG in AR showed nominally significant association with the BRIEF Regulation Index. CONCLUSIONS: Boys with 49,XXXXY displayed higher levels of CpG methylation at regulatory intronic regions in X-linked genes encoding the androgen receptor (AR) and monoamine oxidase A (MAOA), compared to that in boys with 47,XXY and neurotypical boys. Our pilot study results suggest a link between CpG methylation levels and behavior in boys with 49,XXXXY. SN - 1868-7083 UR - https://www.unboundmedicine.com/medline/citation/34210361/DNA_methylation_and_behavioral_dysfunction_in_males_with_47XXY_and_49XXXXY:_a_pilot_study_ DB - PRIME DP - Unbound Medicine ER -