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Genomic characterization of SARS-CoV-2 isolates from patients in Turkey reveals the presence of novel mutations in spike and nsp12 proteins.
J Med Virol. 2021 10; 93(10):6016-6026.JM

Abstract

Novel mutations have been emerging in the genome of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2); consequently, the evolving of more virulent and treatment resistance strains have the potential to increase transmissibility and mortality rates. The characterization of full-length SARS-CoV-2 genomes is critical for understanding the origin and transmission pathways of the virus, as well as identifying mutations that affect the transmissibility and pathogenicity of the virus. We present an analysis of the mutation pattern and clade distribution of full-length SARS-CoV-2 genome sequences obtained from specimens tested at Gazi University Medical Virology Laboratory. Viral RNA was extracted from nasopharyngeal specimens. Next-generation sequencing libraries were prepared and sequenced on Illumina iSeq 100 platform. Raw sequencing data were processed to obtain full-length genome sequences and variant calling was performed to analyze amino acid changes. Clade distribution was determined to understand the phylogenetic background in relation to global data. A total of 293 distinct mutations were identified, of which 152 missense, 124 synonymous, 12 noncoding, and 5 deletions. The most frequent mutations were P323L (nsp12), D614G (ORF2/S), and 2421C>T (5'-untranslated region) found simultaneously in all sequences. Novel mutations were found in nsp12 (V111A, H133R, Y453C, M626K) and ORF2/S (R995G, V1068L). Nine different Pangolin lineages were detected. The most frequently assigned lineage was B.1.1 (17 sequences), followed by B.1 (7 sequences) and B.1.1.36 (3 sequences). Sequence information is essential for revealing genomic diversity. Mutations might have significant functional implications and analysis of these mutations provides valuable information for therapeutic and vaccine development studies. Our findings point to the introduction of the virus into Turkey through various sources and the subsequent spread of several key variants.

Authors+Show Affiliations

Division of Medical Virology, Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.Division of Medical Virology, Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.Division of Medical Virology, Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.Division of Medical Virology, Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.Department of Infectious Diseases and Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.Department of Infectious Diseases and Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.Division of Medical Virology, Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.Division of Medical Virology, Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34241906

Citation

Sahin, Erdem, et al. "Genomic Characterization of SARS-CoV-2 Isolates From Patients in Turkey Reveals the Presence of Novel Mutations in Spike and Nsp12 Proteins." Journal of Medical Virology, vol. 93, no. 10, 2021, pp. 6016-6026.
Sahin E, Bozdayi G, Yigit S, et al. Genomic characterization of SARS-CoV-2 isolates from patients in Turkey reveals the presence of novel mutations in spike and nsp12 proteins. J Med Virol. 2021;93(10):6016-6026.
Sahin, E., Bozdayi, G., Yigit, S., Muftah, H., Dizbay, M., Tunccan, O. G., Fidan, I., & Caglar, K. (2021). Genomic characterization of SARS-CoV-2 isolates from patients in Turkey reveals the presence of novel mutations in spike and nsp12 proteins. Journal of Medical Virology, 93(10), 6016-6026. https://doi.org/10.1002/jmv.27188
Sahin E, et al. Genomic Characterization of SARS-CoV-2 Isolates From Patients in Turkey Reveals the Presence of Novel Mutations in Spike and Nsp12 Proteins. J Med Virol. 2021;93(10):6016-6026. PubMed PMID: 34241906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic characterization of SARS-CoV-2 isolates from patients in Turkey reveals the presence of novel mutations in spike and nsp12 proteins. AU - Sahin,Erdem, AU - Bozdayi,Gulendam, AU - Yigit,Selin, AU - Muftah,Hager, AU - Dizbay,Murat, AU - Tunccan,Ozlem G, AU - Fidan,Isil, AU - Caglar,Kayhan, Y1 - 2021/07/16/ PY - 2021/06/17/received PY - 2021/07/06/accepted PY - 2021/7/10/pubmed PY - 2021/8/20/medline PY - 2021/7/9/entrez KW - SARS-CoV-2 KW - Turkey KW - full-length genome KW - mutation analysis KW - next-generation sequencing KW - phylogenetic tree SP - 6016 EP - 6026 JF - Journal of medical virology JO - J Med Virol VL - 93 IS - 10 N2 - Novel mutations have been emerging in the genome of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2); consequently, the evolving of more virulent and treatment resistance strains have the potential to increase transmissibility and mortality rates. The characterization of full-length SARS-CoV-2 genomes is critical for understanding the origin and transmission pathways of the virus, as well as identifying mutations that affect the transmissibility and pathogenicity of the virus. We present an analysis of the mutation pattern and clade distribution of full-length SARS-CoV-2 genome sequences obtained from specimens tested at Gazi University Medical Virology Laboratory. Viral RNA was extracted from nasopharyngeal specimens. Next-generation sequencing libraries were prepared and sequenced on Illumina iSeq 100 platform. Raw sequencing data were processed to obtain full-length genome sequences and variant calling was performed to analyze amino acid changes. Clade distribution was determined to understand the phylogenetic background in relation to global data. A total of 293 distinct mutations were identified, of which 152 missense, 124 synonymous, 12 noncoding, and 5 deletions. The most frequent mutations were P323L (nsp12), D614G (ORF2/S), and 2421C>T (5'-untranslated region) found simultaneously in all sequences. Novel mutations were found in nsp12 (V111A, H133R, Y453C, M626K) and ORF2/S (R995G, V1068L). Nine different Pangolin lineages were detected. The most frequently assigned lineage was B.1.1 (17 sequences), followed by B.1 (7 sequences) and B.1.1.36 (3 sequences). Sequence information is essential for revealing genomic diversity. Mutations might have significant functional implications and analysis of these mutations provides valuable information for therapeutic and vaccine development studies. Our findings point to the introduction of the virus into Turkey through various sources and the subsequent spread of several key variants. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/34241906/Genomic_characterization_of_SARS_CoV_2_isolates_from_patients_in_Turkey_reveals_the_presence_of_novel_mutations_in_spike_and_nsp12_proteins_ L2 - https://doi.org/10.1002/jmv.27188 DB - PRIME DP - Unbound Medicine ER -