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Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and Its Evaluation in Marker-Assisted Selection.
Plant Dis. 2021 Dec; 105(12):4042-4050.PD

Abstract

Wheat powdery mildew, caused by Blumeria graminis (DC.) Speer f. sp. tritici is a destructive disease seriously threatening yield and quality of common wheat (Triticum aestivum L., 2n=6x=42, AABBDD). Characterization of resistance genes against powdery mildew is useful in parental selection and for developing disease-resistant cultivars. Chinese wheat breeding line KN0816 has superior agronomic performance and resistance to powdery mildew at all growth stages. Genetic analysis using populations of KN0816 crossed with different susceptible parents indicated that a single dominant gene, tentatively designated PmKN0816, conferred seedling resistance to different B. graminis f. sp. tritici isolates. Using a bulked segregant analysis, PmKN0816 was mapped to the Pm6 interval on chromosome arm 2BL using polymorphic markers linked to the cataloged genes Pm6, Pm52, and Pm64, and flanked by the markers CISSR02g-6 and CIT02g-2, both with genetic distances of 0.7 cM. Analysis of closely linked molecular markers indicated that the marker alleles of PmKN0816 differed from those of other powdery mildew resistance genes on 2BL, including Pm6, Pm33, Pm51, Pm64, and PmQ. Based on the genetic and physical locations and response pattern to different B. graminis f. sp. tritici isolates, PmKN0816 is most likely a new powdery mildew resistance gene and possesses effective resistance to all the 14 tested B. graminis f. sp. tritici isolates. In view of the elite agronomic performance of KN0816 combined with the resistance, PmKN0816 is expected to become a valuable resistance gene in wheat breeding. To transfer PmKN0816 to different genetic backgrounds using marker-assisted selection (MAS), closely linked markers of PmKN0816 were evaluated, and four of them (CIT02g-2, CISSR02g-6, CIT02g-10, and CIT02g-17) were confirmed to be applicable for MAS in different genetic backgrounds.

Authors+Show Affiliations

College of Life Sciences, Yantai University, Yantai, Shandong 264005, China.School of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, China.College of Food and Biology, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China.State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, Henan 475004, China.College of Life Sciences, Yantai University, Yantai, Shandong 264005, China.College of Life Sciences, Yantai University, Yantai, Shandong 264005, China.Yantai Jien Biological Science & Technology Ltd, Yantai, Shandong 265100, China.College of Life Sciences, Yantai University, Yantai, Shandong 264005, China. Yantai Jien Biological Science & Technology Ltd, Yantai, Shandong 265100, China.Crop Research Institute, Shandong Academy of Agriculture Sciences, Jinan, Shandong 250100, China.Crop Research Institute, Shandong Academy of Agriculture Sciences, Jinan, Shandong 250100, China.College of Life Sciences, Yantai University, Yantai, Shandong 264005, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34260283

Citation

Wang, Wenrui, et al. "Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and Its Evaluation in Marker-Assisted Selection." Plant Disease, vol. 105, no. 12, 2021, pp. 4042-4050.
Wang W, He H, Gao H, et al. Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and Its Evaluation in Marker-Assisted Selection. Plant Dis. 2021;105(12):4042-4050.
Wang, W., He, H., Gao, H., Xu, H., Song, W., Zhang, X., Zhang, L., Song, J., Liu, C., Liu, K., & Ma, P. (2021). Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and Its Evaluation in Marker-Assisted Selection. Plant Disease, 105(12), 4042-4050. https://doi.org/10.1094/PDIS-05-21-0896-RE
Wang W, et al. Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and Its Evaluation in Marker-Assisted Selection. Plant Dis. 2021;105(12):4042-4050. PubMed PMID: 34260283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the Powdery Mildew Resistance Gene in Wheat Breeding Line KN0816 and Its Evaluation in Marker-Assisted Selection. AU - Wang,Wenrui, AU - He,Huagang, AU - Gao,Huiming, AU - Xu,Hongxing, AU - Song,Wenyue, AU - Zhang,Xu, AU - Zhang,Lipei, AU - Song,Jiancheng, AU - Liu,Cheng, AU - Liu,Kaichang, AU - Ma,Pengtao, Y1 - 2021/12/01/ PY - 2021/7/15/pubmed PY - 2022/1/27/medline PY - 2021/7/14/entrez KW - cereals and grains KW - cultivar/resistance KW - disease management KW - fungi SP - 4042 EP - 4050 JF - Plant disease JO - Plant Dis VL - 105 IS - 12 N2 - Wheat powdery mildew, caused by Blumeria graminis (DC.) Speer f. sp. tritici is a destructive disease seriously threatening yield and quality of common wheat (Triticum aestivum L., 2n=6x=42, AABBDD). Characterization of resistance genes against powdery mildew is useful in parental selection and for developing disease-resistant cultivars. Chinese wheat breeding line KN0816 has superior agronomic performance and resistance to powdery mildew at all growth stages. Genetic analysis using populations of KN0816 crossed with different susceptible parents indicated that a single dominant gene, tentatively designated PmKN0816, conferred seedling resistance to different B. graminis f. sp. tritici isolates. Using a bulked segregant analysis, PmKN0816 was mapped to the Pm6 interval on chromosome arm 2BL using polymorphic markers linked to the cataloged genes Pm6, Pm52, and Pm64, and flanked by the markers CISSR02g-6 and CIT02g-2, both with genetic distances of 0.7 cM. Analysis of closely linked molecular markers indicated that the marker alleles of PmKN0816 differed from those of other powdery mildew resistance genes on 2BL, including Pm6, Pm33, Pm51, Pm64, and PmQ. Based on the genetic and physical locations and response pattern to different B. graminis f. sp. tritici isolates, PmKN0816 is most likely a new powdery mildew resistance gene and possesses effective resistance to all the 14 tested B. graminis f. sp. tritici isolates. In view of the elite agronomic performance of KN0816 combined with the resistance, PmKN0816 is expected to become a valuable resistance gene in wheat breeding. To transfer PmKN0816 to different genetic backgrounds using marker-assisted selection (MAS), closely linked markers of PmKN0816 were evaluated, and four of them (CIT02g-2, CISSR02g-6, CIT02g-10, and CIT02g-17) were confirmed to be applicable for MAS in different genetic backgrounds. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/34260283/Characterization_of_the_Powdery_Mildew_Resistance_Gene_in_Wheat_Breeding_Line_KN0816_and_Its_Evaluation_in_Marker_Assisted_Selection_ L2 - https://apsjournals.apsnet.org/doi/10.1094/PDIS-05-21-0896-RE?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -