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Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor.
Emerg Microbes Infect. 2021 Dec; 10(1):1507-1514.EM

Abstract

Severe respiratory disease coronavirus-2 (SARS-CoV-2) has been the most devastating disease COVID-19 in the century. One of the unsolved scientific questions of SARS-CoV-2 is the animal origin of this virus. Bats and pangolins are recognized as the most probable reservoir hosts that harbour highly similar SARS-CoV-2 related viruses (SARSr-CoV-2). This study identified a novel lineage of SARSr-CoVs, including RaTG15 and seven other viruses, from bats at the same location where we found RaTG13 in 2015. Although RaTG15 and the related viruses share 97.2% amino acid sequence identities with SARS-CoV-2 in the conserved ORF1b region, it only shows less than 77.6% nucleotide identity to all known SARSr-CoVs at the genome level, thus forming a distinct lineage in the Sarbecovirus phylogenetic tree. We found that the RaTG15 receptor-binding domain (RBD) can bind to ACE2 from Rhinolophus affinis, Malayan pangolin, and use it as an entry receptor, except for ACE2 from humans. However, it contains a short deletion and has different key residues responsible for ACE2 binding. In addition, we showed that none of the known viruses in bat SARSr-CoV-2 lineage discovered uses human ACE2 as efficiently as the pangolin-derived SARSr-CoV-2 or some viruses in the SARSr-CoV-1 lineage. Therefore, further systematic and longitudinal studies in bats are needed to prevent future spillover events caused by SARSr-CoVs or to understand the origin of SARS-CoV-2 better.

Authors+Show Affiliations

CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China. University of Chinese Academy of Sciences, Beijing, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China. University of Chinese Academy of Sciences, Beijing, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China. University of Chinese Academy of Sciences, Beijing, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China. University of Chinese Academy of Sciences, Beijing, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China. University of Chinese Academy of Sciences, Beijing, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34263709

Citation

Guo, Hua, et al. "Identification of a Novel Lineage Bat SARS-related Coronaviruses That Use Bat ACE2 Receptor." Emerging Microbes & Infections, vol. 10, no. 1, 2021, pp. 1507-1514.
Guo H, Hu B, Si HR, et al. Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor. Emerg Microbes Infect. 2021;10(1):1507-1514.
Guo, H., Hu, B., Si, H. R., Zhu, Y., Zhang, W., Li, B., Li, A., Geng, R., Lin, H. F., Yang, X. L., Zhou, P., & Shi, Z. L. (2021). Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor. Emerging Microbes & Infections, 10(1), 1507-1514. https://doi.org/10.1080/22221751.2021.1956373
Guo H, et al. Identification of a Novel Lineage Bat SARS-related Coronaviruses That Use Bat ACE2 Receptor. Emerg Microbes Infect. 2021;10(1):1507-1514. PubMed PMID: 34263709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor. AU - Guo,Hua, AU - Hu,Ben, AU - Si,Hao-Rui, AU - Zhu,Yan, AU - Zhang,Wei, AU - Li,Bei, AU - Li,Ang, AU - Geng,Rong, AU - Lin,Hao-Feng, AU - Yang,Xing-Lou, AU - Zhou,Peng, AU - Shi,Zheng-Li, PY - 2021/7/16/pubmed PY - 2021/8/10/medline PY - 2021/7/15/entrez KW - ACE2 KW - SARS-related coronavirus KW - bat KW - novel lineage KW - reservoir host SP - 1507 EP - 1514 JF - Emerging microbes & infections JO - Emerg Microbes Infect VL - 10 IS - 1 N2 - Severe respiratory disease coronavirus-2 (SARS-CoV-2) has been the most devastating disease COVID-19 in the century. One of the unsolved scientific questions of SARS-CoV-2 is the animal origin of this virus. Bats and pangolins are recognized as the most probable reservoir hosts that harbour highly similar SARS-CoV-2 related viruses (SARSr-CoV-2). This study identified a novel lineage of SARSr-CoVs, including RaTG15 and seven other viruses, from bats at the same location where we found RaTG13 in 2015. Although RaTG15 and the related viruses share 97.2% amino acid sequence identities with SARS-CoV-2 in the conserved ORF1b region, it only shows less than 77.6% nucleotide identity to all known SARSr-CoVs at the genome level, thus forming a distinct lineage in the Sarbecovirus phylogenetic tree. We found that the RaTG15 receptor-binding domain (RBD) can bind to ACE2 from Rhinolophus affinis, Malayan pangolin, and use it as an entry receptor, except for ACE2 from humans. However, it contains a short deletion and has different key residues responsible for ACE2 binding. In addition, we showed that none of the known viruses in bat SARSr-CoV-2 lineage discovered uses human ACE2 as efficiently as the pangolin-derived SARSr-CoV-2 or some viruses in the SARSr-CoV-1 lineage. Therefore, further systematic and longitudinal studies in bats are needed to prevent future spillover events caused by SARSr-CoVs or to understand the origin of SARS-CoV-2 better. SN - 2222-1751 UR - https://www.unboundmedicine.com/medline/citation/34263709/Identification_of_a_novel_lineage_bat_SARS_related_coronaviruses_that_use_bat_ACE2_receptor_ L2 - https://www.tandfonline.com/doi/full/10.1080/22221751.2021.1956373 DB - PRIME DP - Unbound Medicine ER -