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Protective effect of the effective part of Andrographis paniculata (Burm.f.) Nees on PM2.5-induced lung injury in rats by modulating the NF-κB pathway.
J Ethnopharmacol. 2021 Nov 15; 280:114420.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Andrographis paniculata (Burm.f.) Nees, a traditional Chinese herb, has been widely used in various Asian countries as a treatment for upper respiratory tract infections for centuries.

AIM OF THE STUDY

Continuous inhalation of fine particulate matter (PM2.5) may induce various respiratory diseases. This study elucidated the protective effect of the effective part of Andrographis paniculata (Burm.f.) Nees (AEP) against PM2.5-induced lung injury and detailed the underlying mechanism.

MATERIALS AND METHODS

Male Wistar rats were orally administered 0.5% sodium carboxymethylcellulose (CMC-Na), andrographolide (AG) (200 mg/kg) and AEP (100 mg/kg, 200 mg/kg and 400 mg/kg) once a day for 28 days. The rats were intratracheally instilled with PM2.5 suspension (8 mg/kg) every other day beginning on the 24th day for a total of 3 times. On the 29th day, bronchoalveolar lavage fluid (BALF) was collected to analyze the levels of lactate dehydrogenase (LDH), acid phosphatase (ACP), alkaline phosphatase (AKP), total proteins (TP), tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6). Hematoxylin & eosin staining was conducted to evaluate the pathological changes in the lung tissues. The protein expression of NF-κB p65 in the lung tissues was analyzed by immunohistochemistry staining. Moreover, the nuclear translocation of NF-κB p65 and the phosphorylation of IκBα were analyzed by western blotting.

RESULTS

PM2.5 exposure caused lung toxicity, which was characterized by pathological injury and increased levels of LDH, ACP, AKP and TP in BALF. Meanwhile, PM2.5 exposure induced lung inflammatory response, including infiltration of inflammatory cells and increased levels of inflammatory factors, such as TNF-α and IL-6 in BALF. AEP treatment significantly ameliorated the PM2.5-induced lung toxicity and the inflammatory response in rats. Moreover, AEP significantly inhibited the PM2.5-induced upregulation of NF-κB p65 protein expression, phosphorylation of IκBα and nuclear translocation of NF-κB p65 in lung tissue. Compared to AG, AEP exhibited a better ability to alleviate PM2.5-induced pathological damage and decrease the TP level in the BALF.

CONCLUSION

AEP could be used to improve PM2.5-induced lung injury by modulating the NF-κB pathway, and multicomponent therapy with traditional Chinese medicine may be more effective than single-drug therapy.

Authors+Show Affiliations

Institute of Pharmacy, College of Pharmacy, Henan University, Kaifeng, 475000, China. Electronic address: 18739971617@163.com.Institute of Pharmacy, College of Pharmacy, Henan University, Kaifeng, 475000, China. Electronic address: 983683193@qq.com.Henan Provincial Institute of Food and Drug Control, Zhengzhou, 450008, China. Electronic address: 754977817@qq.com.Institute of Pharmacy, College of Pharmacy, Henan University, Kaifeng, 475000, China. Electronic address: 425213147@qq.com.Institute of Pharmacy, College of Pharmacy, Henan University, Kaifeng, 475000, China. Electronic address: 17856776263@163.com.Institute of Pharmacy, College of Pharmacy, Henan University, Kaifeng, 475000, China. Electronic address: 522841295@qq.com.Institute of Pharmacy, College of Pharmacy, Henan University, Kaifeng, 475000, China. Electronic address: 54484538@qq.com.Institute of Pharmacy, College of Pharmacy, Henan University, Kaifeng, 475000, China. Electronic address: hang@henu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34271116

Citation

Yao, Hailu, et al. "Protective Effect of the Effective Part of Andrographis Paniculata (Burm.f.) Nees On PM2.5-induced Lung Injury in Rats By Modulating the NF-κB Pathway." Journal of Ethnopharmacology, vol. 280, 2021, p. 114420.
Yao H, Zhao J, Zhu L, et al. Protective effect of the effective part of Andrographis paniculata (Burm.f.) Nees on PM2.5-induced lung injury in rats by modulating the NF-κB pathway. J Ethnopharmacol. 2021;280:114420.
Yao, H., Zhao, J., Zhu, L., Xie, Y., Zhao, N., Yao, R., Sun, H., & Han, G. (2021). Protective effect of the effective part of Andrographis paniculata (Burm.f.) Nees on PM2.5-induced lung injury in rats by modulating the NF-κB pathway. Journal of Ethnopharmacology, 280, 114420. https://doi.org/10.1016/j.jep.2021.114420
Yao H, et al. Protective Effect of the Effective Part of Andrographis Paniculata (Burm.f.) Nees On PM2.5-induced Lung Injury in Rats By Modulating the NF-κB Pathway. J Ethnopharmacol. 2021 Nov 15;280:114420. PubMed PMID: 34271116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of the effective part of Andrographis paniculata (Burm.f.) Nees on PM2.5-induced lung injury in rats by modulating the NF-κB pathway. AU - Yao,Hailu, AU - Zhao,Junli, AU - Zhu,Lingjia, AU - Xie,Yudan, AU - Zhao,Nana, AU - Yao,Ruiqi, AU - Sun,Huan, AU - Han,Guang, Y1 - 2021/07/13/ PY - 2021/05/31/received PY - 2021/07/04/revised PY - 2021/07/12/accepted PY - 2021/7/17/pubmed PY - 2022/1/12/medline PY - 2021/7/16/entrez KW - Andrographolide KW - Lung injury KW - NF-κB KW - PM(2.5) KW - The effective part of Andrographis paniculata (Burm.f.) Nees SP - 114420 EP - 114420 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 280 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Andrographis paniculata (Burm.f.) Nees, a traditional Chinese herb, has been widely used in various Asian countries as a treatment for upper respiratory tract infections for centuries. AIM OF THE STUDY: Continuous inhalation of fine particulate matter (PM2.5) may induce various respiratory diseases. This study elucidated the protective effect of the effective part of Andrographis paniculata (Burm.f.) Nees (AEP) against PM2.5-induced lung injury and detailed the underlying mechanism. MATERIALS AND METHODS: Male Wistar rats were orally administered 0.5% sodium carboxymethylcellulose (CMC-Na), andrographolide (AG) (200 mg/kg) and AEP (100 mg/kg, 200 mg/kg and 400 mg/kg) once a day for 28 days. The rats were intratracheally instilled with PM2.5 suspension (8 mg/kg) every other day beginning on the 24th day for a total of 3 times. On the 29th day, bronchoalveolar lavage fluid (BALF) was collected to analyze the levels of lactate dehydrogenase (LDH), acid phosphatase (ACP), alkaline phosphatase (AKP), total proteins (TP), tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6). Hematoxylin & eosin staining was conducted to evaluate the pathological changes in the lung tissues. The protein expression of NF-κB p65 in the lung tissues was analyzed by immunohistochemistry staining. Moreover, the nuclear translocation of NF-κB p65 and the phosphorylation of IκBα were analyzed by western blotting. RESULTS: PM2.5 exposure caused lung toxicity, which was characterized by pathological injury and increased levels of LDH, ACP, AKP and TP in BALF. Meanwhile, PM2.5 exposure induced lung inflammatory response, including infiltration of inflammatory cells and increased levels of inflammatory factors, such as TNF-α and IL-6 in BALF. AEP treatment significantly ameliorated the PM2.5-induced lung toxicity and the inflammatory response in rats. Moreover, AEP significantly inhibited the PM2.5-induced upregulation of NF-κB p65 protein expression, phosphorylation of IκBα and nuclear translocation of NF-κB p65 in lung tissue. Compared to AG, AEP exhibited a better ability to alleviate PM2.5-induced pathological damage and decrease the TP level in the BALF. CONCLUSION: AEP could be used to improve PM2.5-induced lung injury by modulating the NF-κB pathway, and multicomponent therapy with traditional Chinese medicine may be more effective than single-drug therapy. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/34271116/Protective_effect_of_the_effective_part_of_Andrographis_paniculata__Burm_f___Nees_on_PM2_5_induced_lung_injury_in_rats_by_modulating_the_NF_κB_pathway_ DB - PRIME DP - Unbound Medicine ER -