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Simultaneous inactivation of multidrug-resistant Escherichia coli and enterococci by peracetic acid in urban wastewater: Exposure-based kinetics and comparison with chlorine.
Water Res. 2021 Sep 01; 202:117403.WR

Abstract

The presence of antibiotic resistance in wastewater sparked a great interest in investigating the inactivation of antibiotic-resistant bacteria by disinfecting agents. In this study, the inactivation kinetics of multidrug-resistant E. coli and enterococci by an emerging environmentally-friendly disinfectant, peracetic acid (PAA), in wastewater and phosphate buffer at pH 6.5 and pH 7.5, were characterized. It was demonstrated that the inactivation of the studied multidrug-resistant bacteria was governed by their exposure to PAA, i.e., integral of the PAA concentration over time (integral CT or ICT). Both regimes of the PAA inactivation of bacteria, i.e., initial resistance followed by a faster inactivation, were described well by an ICT-based Chick-Watson inactivation kinetic model. In wastewater at pH 7.5, the model-predicted ICT requirements showed that the multidrug-resistant enterococci were less susceptible to PAA than E. coli, e.g., to achieve a 3-log reduction, an ICT of 32.7 mg min/L and 23.4 mg min/L was needed, respectively. No regrowth of the studied bacteria was observed after 72 h from PAA disinfection at 25 ± 1 °C. Soluble constituents of wastewater decreased the PAA inactivation of both multidrug-resistant bacteria, i.e., higher inactivation was observed in phosphate buffer than wastewater at the same pH of 7.5. In phosphate buffer, a lower pH of 6.5 resulted in higher inactivation of multidrug-resistant E. coli compared with pH 7.5, but it did not affect the PAA inactivation of multidrug-resistant enterococci. A comparison with the most commonly used chemical disinfectant, chlorine, showed higher inactivation of both multidrug-resistant bacteria by chlorine and higher chlorine decay than PAA. The results of the present study may have implications in designing a PAA disinfection process, aiming at controlling antibiotic resistance, in terms of selecting a suitable fecal indicator and optimizing disinfectant dosing.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Balachandran S
Institute of Urban Water Management, Department of Hydrosciences, Technische Universität Dresden (TU Dresden), Zellescher Weg 18, 01069 Dresden, Germany.
Charamba LVC
Institute of Urban Water Management, Department of Hydrosciences, Technische Universität Dresden (TU Dresden), Zellescher Weg 18, 01069 Dresden, Germany.
Manoli K
Nireas-International Water Research Center, University of Cyprus, P.O. Box 20537, CY-1678 Nicosia, Cyprus; Department of Civil and Environmental Engineering, University of Cyprus, P.O. Box 20537, CY-1678 Nicosia, Cyprus. Electronic address: manoli.kyriakos@ucy.ac.cy.
Karaolia P
Nireas-International Water Research Center, University of Cyprus, P.O. Box 20537, CY-1678 Nicosia, Cyprus.
Caucci S
Institute for Integrated Management of Material Fluxes and of Resources (UNU-FLORES), United Nations University, Ammonstrasse 74, 01067 Dresden, Germany.
Fatta-Kassinos D
Nireas-International Water Research Center, University of Cyprus, P.O. Box 20537, CY-1678 Nicosia, Cyprus; Department of Civil and Environmental Engineering, University of Cyprus, P.O. Box 20537, CY-1678 Nicosia, Cyprus. Electronic address: dfatta@ucy.ac.cy.

MeSH

ChlorineDisinfectantsDisinfectionEnterococcusEscherichia coliKineticsPeracetic AcidWastewaterWater Purification

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34274900

Citation

Balachandran, Sanjana, et al. "Simultaneous Inactivation of Multidrug-resistant Escherichia Coli and Enterococci By Peracetic Acid in Urban Wastewater: Exposure-based Kinetics and Comparison With Chlorine." Water Research, vol. 202, 2021, p. 117403.
Balachandran S, Charamba LVC, Manoli K, et al. Simultaneous inactivation of multidrug-resistant Escherichia coli and enterococci by peracetic acid in urban wastewater: Exposure-based kinetics and comparison with chlorine. Water Res. 2021;202:117403.
Balachandran, S., Charamba, L. V. C., Manoli, K., Karaolia, P., Caucci, S., & Fatta-Kassinos, D. (2021). Simultaneous inactivation of multidrug-resistant Escherichia coli and enterococci by peracetic acid in urban wastewater: Exposure-based kinetics and comparison with chlorine. Water Research, 202, 117403. https://doi.org/10.1016/j.watres.2021.117403
Balachandran S, et al. Simultaneous Inactivation of Multidrug-resistant Escherichia Coli and Enterococci By Peracetic Acid in Urban Wastewater: Exposure-based Kinetics and Comparison With Chlorine. Water Res. 2021 Sep 1;202:117403. PubMed PMID: 34274900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous inactivation of multidrug-resistant Escherichia coli and enterococci by peracetic acid in urban wastewater: Exposure-based kinetics and comparison with chlorine. AU - Balachandran,Sanjana, AU - Charamba,Livia V C, AU - Manoli,Kyriakos, AU - Karaolia,Popi, AU - Caucci,Serena, AU - Fatta-Kassinos,Despo, Y1 - 2021/07/04/ PY - 2021/04/28/received PY - 2021/06/23/revised PY - 2021/06/29/accepted PY - 2021/7/19/pubmed PY - 2021/9/7/medline PY - 2021/7/18/entrez KW - Antibiotic resistance KW - Bacteria KW - Chlorine KW - Disinfection KW - Peracetic acid KW - Wastewater SP - 117403 EP - 117403 JF - Water research JO - Water Res VL - 202 N2 - The presence of antibiotic resistance in wastewater sparked a great interest in investigating the inactivation of antibiotic-resistant bacteria by disinfecting agents. In this study, the inactivation kinetics of multidrug-resistant E. coli and enterococci by an emerging environmentally-friendly disinfectant, peracetic acid (PAA), in wastewater and phosphate buffer at pH 6.5 and pH 7.5, were characterized. It was demonstrated that the inactivation of the studied multidrug-resistant bacteria was governed by their exposure to PAA, i.e., integral of the PAA concentration over time (integral CT or ICT). Both regimes of the PAA inactivation of bacteria, i.e., initial resistance followed by a faster inactivation, were described well by an ICT-based Chick-Watson inactivation kinetic model. In wastewater at pH 7.5, the model-predicted ICT requirements showed that the multidrug-resistant enterococci were less susceptible to PAA than E. coli, e.g., to achieve a 3-log reduction, an ICT of 32.7 mg min/L and 23.4 mg min/L was needed, respectively. No regrowth of the studied bacteria was observed after 72 h from PAA disinfection at 25 ± 1 °C. Soluble constituents of wastewater decreased the PAA inactivation of both multidrug-resistant bacteria, i.e., higher inactivation was observed in phosphate buffer than wastewater at the same pH of 7.5. In phosphate buffer, a lower pH of 6.5 resulted in higher inactivation of multidrug-resistant E. coli compared with pH 7.5, but it did not affect the PAA inactivation of multidrug-resistant enterococci. A comparison with the most commonly used chemical disinfectant, chlorine, showed higher inactivation of both multidrug-resistant bacteria by chlorine and higher chlorine decay than PAA. The results of the present study may have implications in designing a PAA disinfection process, aiming at controlling antibiotic resistance, in terms of selecting a suitable fecal indicator and optimizing disinfectant dosing. SN - 1879-2448 UR - https://www.unboundmedicine.com/medline/citation/34274900/Simultaneous_inactivation_of_multidrug_resistant_Escherichia_coli_and_enterococci_by_peracetic_acid_in_urban_wastewater:_Exposure_based_kinetics_and_comparison_with_chlorine_ DB - PRIME DP - Unbound Medicine ER -