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Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides.
Mem Inst Oswaldo Cruz. 2021; 116:e200587.MI

Abstract

BACKGROUND

The inappropriate use of antibiotics has led to the accelerated growth of resistance to antibiotics. The search for new therapeutic strategies (i.e., antimicrobial peptides-AMPs) has thus become a pressing need.

OBJECTIVE

Characterising and evaluating Sarconesiopsis magellanica larval fat body-derived AMPs.

METHODS

Fat body extracts were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC); mass spectrometry was used for characterising the primary structure of the AMPs so found. ProtParam (Expasy) was used for analysing the AMPs' physico-chemical properties. Synthetic AMPs' antibacterial activity was evaluated.

FINDINGS

Four new AMPs were obtained and called sarconesin III, IV, V and VI. Sarconesin III had an α-helix structure and sarconesins IV, V and VI had linear formations. Oligomer prediction highlighted peptide-peptide interactions, suggesting that sarconesins III, V and VI could form self-aggregations when in contact with the microbial membrane. AMPs synthesised from their native molecules' sequences had potent activity against Gram-positive bacteria and, to a lesser extent, against Gram-negative and drug-resistant bacteria. Sarconesin VI was the most efficient AMP. None of the four synthetic AMPs had a cytotoxic effect.

MAIN CONCLUSIONS

S. magellanica larval fat body-derived antimicrobial peptides are an important source of AMPs and could be used in different antimicrobial therapies and overcoming bacterial resistance.

Links

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Authors+Show Affiliations

Pérez C
Universidad Antonio Nariño, Facultad de Medicina Veterinaria, Bogotá, Colombia.
Díaz-Roa A
Instituto Butantan, Laboratório de Toxinología Aplicada, São Paulo, SP, Brasil. Universidad Nacional Abierta y a Distancia, Escuela de Ciencias Agrícolas, Pecuarias y de Medio Ambiente, Bogotá, Colombia.
Bernal Y
Universidad Antonio Nariño, Facultad de Ciencias, Bogotá, Colombia.
Arenas NE
Universidad Antonio Nariño, Facultad de Ciencias, Bogotá, Colombia.
Kalume DE
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório Interdisciplinar de Pesquisas Médicas, Rio de Janeiro, RJ, Brasil.
Côrtes LMC
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Rio de Janeiro, RJ, Brasil.
da Silva Junior PI
Instituto Butantan, Laboratório de Toxinología Aplicada, São Paulo, SP, Brasil.
Varela Y
Fundación Instituto de Inmunología de Colombia, Molecular Biology and Immunology Department, Bogotá, Colombia.
Patarroyo MA
Fundación Instituto de Inmunología de Colombia, Molecular Biology and Immunology Department, Bogotá, Colombia. Universidad Nacional de Colombia, Faculty of Medicine, Microbiology Department, Bogotá, Colombia. Universidad Santo Tomás, Health Sciences Division, Bogotá, Colombia.
Torres O
Universidad Antonio Nariño, Facultad de Medicina Veterinaria, Bogotá, Colombia.
Bello FJ
Universidad de La Salle, Facultad de Ciencias Agropecuarias, Programa de Medicina Veterinaria, Bogotá, Colombia.

MeSH

AnimalsAnti-Bacterial AgentsCalliphoridaeDipteraFat BodyLarvaMicrobial Sensitivity TestsPore Forming Cytotoxic Proteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34287503

Citation

Pérez, Cindy, et al. "Characterising Four Sarconesiopsis Magellanica (Diptera: Calliphoridae) Larval Fat Body-derived Antimicrobial Peptides." Memorias Do Instituto Oswaldo Cruz, vol. 116, 2021, pp. e200587.
Pérez C, Díaz-Roa A, Bernal Y, et al. Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. Mem Inst Oswaldo Cruz. 2021;116:e200587.
Pérez, C., Díaz-Roa, A., Bernal, Y., Arenas, N. E., Kalume, D. E., Côrtes, L. M. C., da Silva Junior, P. I., Varela, Y., Patarroyo, M. A., Torres, O., & Bello, F. J. (2021). Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. Memorias Do Instituto Oswaldo Cruz, 116, e200587. https://doi.org/10.1590/0074-02760200587
Pérez C, et al. Characterising Four Sarconesiopsis Magellanica (Diptera: Calliphoridae) Larval Fat Body-derived Antimicrobial Peptides. Mem Inst Oswaldo Cruz. 2021;116:e200587. PubMed PMID: 34287503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides. AU - Pérez,Cindy, AU - Díaz-Roa,Andrea, AU - Bernal,Yuly, AU - Arenas,Nelson E, AU - Kalume,Dario Eluan, AU - Côrtes,Luzia Monteiro de Castro, AU - da Silva Junior,Pedro I, AU - Varela,Yahson, AU - Patarroyo,Manuel A, AU - Torres,Orlando, AU - Bello,Felio J, Y1 - 2021/07/16/ PY - 2020/11/14/received PY - 2021/06/07/accepted PY - 2021/7/21/entrez PY - 2021/7/22/pubmed PY - 2021/7/24/medline SP - e200587 EP - e200587 JF - Memorias do Instituto Oswaldo Cruz JO - Mem Inst Oswaldo Cruz VL - 116 N2 - BACKGROUND: The inappropriate use of antibiotics has led to the accelerated growth of resistance to antibiotics. The search for new therapeutic strategies (i.e., antimicrobial peptides-AMPs) has thus become a pressing need. OBJECTIVE: Characterising and evaluating Sarconesiopsis magellanica larval fat body-derived AMPs. METHODS: Fat body extracts were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC); mass spectrometry was used for characterising the primary structure of the AMPs so found. ProtParam (Expasy) was used for analysing the AMPs' physico-chemical properties. Synthetic AMPs' antibacterial activity was evaluated. FINDINGS: Four new AMPs were obtained and called sarconesin III, IV, V and VI. Sarconesin III had an α-helix structure and sarconesins IV, V and VI had linear formations. Oligomer prediction highlighted peptide-peptide interactions, suggesting that sarconesins III, V and VI could form self-aggregations when in contact with the microbial membrane. AMPs synthesised from their native molecules' sequences had potent activity against Gram-positive bacteria and, to a lesser extent, against Gram-negative and drug-resistant bacteria. Sarconesin VI was the most efficient AMP. None of the four synthetic AMPs had a cytotoxic effect. MAIN CONCLUSIONS: S. magellanica larval fat body-derived antimicrobial peptides are an important source of AMPs and could be used in different antimicrobial therapies and overcoming bacterial resistance. SN - 1678-8060 UR - https://www.unboundmedicine.com/medline/citation/34287503/Characterising_four_Sarconesiopsis_magellanica__Diptera:_Calliphoridae__larval_fat_body_derived_antimicrobial_peptides_ DB - PRIME DP - Unbound Medicine ER -