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Quantification of fentanyl analogs in oral fluid using LC-QTOF-MS.
J Forensic Sci. 2021 Sep; 66(5):1871-1878.JF

Abstract

Oral fluid is a valuable alternative matrix for forensic toxicologists due to ease of observed collection, limited biohazardous exposure, and indications of recent drug use. Limited information is available for fentanyl analog prevalence, interpretation, or analysis in oral fluid. With increasing numbers of fentanyl-related driving under the influence of drug (DUID) cases appearing in the United States, the development of detection methods is critical. The purpose of the present study was to develop and validate a quantitative method for fentanyl analogs in oral fluid (collected via Quantisal™) using liquid chromatography-quadrupole-time-of-flight-mass spectrometry (LC-QTOF-MS). Validation resulted in limits of detection and quantification ranging from 0.5 to 1 ng/mL. Established linear range was 1-100 ng/mL for all analytes, except acetyl fentanyl at 0.5-100 ng/mL (R2 > 0.994). Within- and between-run precision and bias were considered acceptable with maximum values of ±15.2%CV and ±14.1%, respectively. Matrix effects exhibited ionization enhancement for all analytes with intensified enhancement at a low concentration (9.3-47.4%). No interferences or carryover was observed. Fentanyl analogs were stable in processed extracts stored in the autosampler (4⁰ C) for 48h. The validated method was used to quantify fentanyl analogs in authentic oral fluid samples (n=17) from probationers/parolees. Fentanyl and 4-ANPP concentrations were 1.0-104.5 ng/mL and 1.2-5.7 ng/mL, respectively.

Authors+Show Affiliations

Department of Forensic Science, College of Criminal Justice, Sam Houston State University, Huntsville, TX, USA.Department of Forensic Science, College of Criminal Justice, Sam Houston State University, Huntsville, TX, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34287912

Citation

Palmquist, Kaitlyn B., and Madeleine J. Swortwood. "Quantification of Fentanyl Analogs in Oral Fluid Using LC-QTOF-MS." Journal of Forensic Sciences, vol. 66, no. 5, 2021, pp. 1871-1878.
Palmquist KB, Swortwood MJ. Quantification of fentanyl analogs in oral fluid using LC-QTOF-MS. J Forensic Sci. 2021;66(5):1871-1878.
Palmquist, K. B., & Swortwood, M. J. (2021). Quantification of fentanyl analogs in oral fluid using LC-QTOF-MS. Journal of Forensic Sciences, 66(5), 1871-1878. https://doi.org/10.1111/1556-4029.14813
Palmquist KB, Swortwood MJ. Quantification of Fentanyl Analogs in Oral Fluid Using LC-QTOF-MS. J Forensic Sci. 2021;66(5):1871-1878. PubMed PMID: 34287912.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of fentanyl analogs in oral fluid using LC-QTOF-MS. AU - Palmquist,Kaitlyn B, AU - Swortwood,Madeleine J, Y1 - 2021/07/20/ PY - 2021/07/06/revised PY - 2021/05/24/received PY - 2021/07/08/accepted PY - 2021/7/22/pubmed PY - 2021/9/16/medline PY - 2021/7/21/entrez KW - alternative matrix KW - fentanyl analogs KW - forensic toxicology KW - liquid-chromatography-quadrupole-time-of-flight-mass spectrometry (LC-QTOF-MS) KW - novel synthetic opioids KW - oral fluid SP - 1871 EP - 1878 JF - Journal of forensic sciences JO - J Forensic Sci VL - 66 IS - 5 N2 - Oral fluid is a valuable alternative matrix for forensic toxicologists due to ease of observed collection, limited biohazardous exposure, and indications of recent drug use. Limited information is available for fentanyl analog prevalence, interpretation, or analysis in oral fluid. With increasing numbers of fentanyl-related driving under the influence of drug (DUID) cases appearing in the United States, the development of detection methods is critical. The purpose of the present study was to develop and validate a quantitative method for fentanyl analogs in oral fluid (collected via Quantisal™) using liquid chromatography-quadrupole-time-of-flight-mass spectrometry (LC-QTOF-MS). Validation resulted in limits of detection and quantification ranging from 0.5 to 1 ng/mL. Established linear range was 1-100 ng/mL for all analytes, except acetyl fentanyl at 0.5-100 ng/mL (R2 > 0.994). Within- and between-run precision and bias were considered acceptable with maximum values of ±15.2%CV and ±14.1%, respectively. Matrix effects exhibited ionization enhancement for all analytes with intensified enhancement at a low concentration (9.3-47.4%). No interferences or carryover was observed. Fentanyl analogs were stable in processed extracts stored in the autosampler (4⁰ C) for 48h. The validated method was used to quantify fentanyl analogs in authentic oral fluid samples (n=17) from probationers/parolees. Fentanyl and 4-ANPP concentrations were 1.0-104.5 ng/mL and 1.2-5.7 ng/mL, respectively. SN - 1556-4029 UR - https://www.unboundmedicine.com/medline/citation/34287912/Quantification_of_fentanyl_analogs_in_oral_fluid_using_LC_QTOF_MS_ L2 - https://doi.org/10.1111/1556-4029.14813 DB - PRIME DP - Unbound Medicine ER -