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Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies.
Sci Rep. 2021 07 21; 11(1):14926.SR

Abstract

Infection with SARS-CoV-2, the Betacoronavirus, caused a pandemic that affected the globe negatively. The gold method, RT-PCR, can detect SARS-CoV-2 but it is time-consuming and needs sophisticated equipment and professional personnel. On the other hand, rapid tests offer fast results and can detect anti-SARS-CoV-2 antibodies (Abs). The aim of this study is to develop a new rapid and cost-effective method for the detection of anti-SARS-CoV-2 IgG/IgM Abs. A new top-loading detection device was developed and composed of a small piece of plastic (25 × 25 × 0.5 mm) with an opening in the center, a piece of nitrocellulose (NC) membrane enough to block the opening from one side and adhesive tape to affix the NC to the plastic piece. The NC is blotted with anti-human IgG/IgM and rabbit serum. The device was evaluated against a commercially available IgG/IgM ELISA detection kit using normal, Covid-19-positive, HCV, HBV, and Cytomegalovirus-positive sera. Outcomes demonstrated simplicity, reproducibility, and accuracy of the new device and results can be obtained in less than 5 min. We anticipate our developed assay method to be used widely in point of care before deciding on the use of expensive nucleic acid assays.

Authors+Show Affiliations

Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia. essamebrahim@hotmail.com. Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia. essamebrahim@hotmail.com. Blood Products Quality Control and Research Department, National Organization for Research and Control of Biologicals, Cairo, Egypt. essamebrahim@hotmail.com.Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia. Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia. Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia.Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia. Department of Microbiology, National Organization for Drug Control and Research (NODCAR), Cairo, Egypt.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34290350

Citation

Ibrahim, Essam H., et al. "Development of Rapid and Cost-effective Top-loading Device for the Detection of anti-SARS-CoV-2 IgG/IgM Antibodies." Scientific Reports, vol. 11, no. 1, 2021, p. 14926.
Ibrahim EH, Ghramh HA, Kilany M. Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies. Sci Rep. 2021;11(1):14926.
Ibrahim, E. H., Ghramh, H. A., & Kilany, M. (2021). Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies. Scientific Reports, 11(1), 14926. https://doi.org/10.1038/s41598-021-94444-6
Ibrahim EH, Ghramh HA, Kilany M. Development of Rapid and Cost-effective Top-loading Device for the Detection of anti-SARS-CoV-2 IgG/IgM Antibodies. Sci Rep. 2021 07 21;11(1):14926. PubMed PMID: 34290350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies. AU - Ibrahim,Essam H, AU - Ghramh,Hamed A, AU - Kilany,Mona, Y1 - 2021/07/21/ PY - 2021/01/23/received PY - 2021/07/12/accepted PY - 2021/7/22/entrez PY - 2021/7/23/pubmed PY - 2021/8/11/medline SP - 14926 EP - 14926 JF - Scientific reports JO - Sci Rep VL - 11 IS - 1 N2 - Infection with SARS-CoV-2, the Betacoronavirus, caused a pandemic that affected the globe negatively. The gold method, RT-PCR, can detect SARS-CoV-2 but it is time-consuming and needs sophisticated equipment and professional personnel. On the other hand, rapid tests offer fast results and can detect anti-SARS-CoV-2 antibodies (Abs). The aim of this study is to develop a new rapid and cost-effective method for the detection of anti-SARS-CoV-2 IgG/IgM Abs. A new top-loading detection device was developed and composed of a small piece of plastic (25 × 25 × 0.5 mm) with an opening in the center, a piece of nitrocellulose (NC) membrane enough to block the opening from one side and adhesive tape to affix the NC to the plastic piece. The NC is blotted with anti-human IgG/IgM and rabbit serum. The device was evaluated against a commercially available IgG/IgM ELISA detection kit using normal, Covid-19-positive, HCV, HBV, and Cytomegalovirus-positive sera. Outcomes demonstrated simplicity, reproducibility, and accuracy of the new device and results can be obtained in less than 5 min. We anticipate our developed assay method to be used widely in point of care before deciding on the use of expensive nucleic acid assays. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/34290350/Development_of_rapid_and_cost-effective_top-loading_device_for_the_detection_of_anti-SARS-CoV-2_IgG/IgM_antibodies. L2 - https://doi.org/10.1038/s41598-021-94444-6 DB - PRIME DP - Unbound Medicine ER -