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Molecular evidence suggesting the persistence of residual SARS-CoV-2 and immune responses in the placentas of pregnant patients recovered from COVID-19.
Cell Prolif. 2021 Jul 22 [Online ahead of print]CP

Abstract

OBJECTIVES

Recent studies have shown the presence of SARS-CoV-2 in the tissues of clinically recovered patients and persistent immune symptoms in discharged patients for up to several months. Pregnant patients were shown to be a high-risk group for COVID-19. Based on these findings, we assessed SARS-CoV-2 nucleic acid and protein retention in the placentas of pregnant women who had fully recovered from COVID-19 and cytokine fluctuations in maternal and foetal tissues.

MATERIALS AND METHODS

Remnant SARS-CoV-2 in the term placenta was detected using nucleic acid amplification and immunohistochemical staining of the SARS-CoV-2 protein. The infiltration of CD14+ macrophages into the placental villi was detected by immunostaining. The cytokines in the placenta, maternal plasma, neonatal umbilical cord, cord blood and amniotic fluid specimens at delivery were profiled using the Luminex assay.

RESULTS

Residual SARS-CoV-2 nucleic acid and protein were detected in the term placentas of recovered pregnant women. The infiltration of CD14+ macrophages into the placental villi of the recovered pregnant women was higher than that in the controls. Furthermore, the cytokine levels in the placenta, maternal plasma, neonatal umbilical cord, cord blood and amniotic fluid specimens fluctuated significantly.

CONCLUSIONS

Our study showed that SARS-CoV-2 nucleic acid (in one patient) and protein (in five patients) were present in the placentas of clinically recovered pregnant patients for more than 3 months after diagnosis. The immune responses induced by the virus may lead to prolonged and persistent symptoms in the maternal plasma, placenta, umbilical cord, cord blood and amniotic fluid.

Authors+Show Affiliations

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. University of Chinese Academy of Sciences, Beijing, China. Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. University of Chinese Academy of Sciences, Beijing, China. Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Obstetrics, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Obstetrics, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Obstetrics, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, China.Department of Obstetrics and Gynecology, People's Hospital of Huangmei Country, Huanggang City, China.Zhongnan Hospital, Wuhan University, Wuhan, China.State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.Department of Obstetrics, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. University of Chinese Academy of Sciences, Beijing, China. Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34291856

Citation

Wu, Hao, et al. "Molecular Evidence Suggesting the Persistence of Residual SARS-CoV-2 and Immune Responses in the Placentas of Pregnant Patients Recovered From COVID-19." Cell Proliferation, 2021, pp. e13091.
Wu H, Liao S, Wang Y, et al. Molecular evidence suggesting the persistence of residual SARS-CoV-2 and immune responses in the placentas of pregnant patients recovered from COVID-19. Cell Prolif. 2021.
Wu, H., Liao, S., Wang, Y., Guo, M., Lin, X., Wu, J., Wang, R., Lv, D., Wu, D., He, M., Hu, B., Long, R., Peng, J., Yang, H., Yin, H., Wang, X., Huang, Z., Lan, K., Zhou, Y., ... Wang, H. (2021). Molecular evidence suggesting the persistence of residual SARS-CoV-2 and immune responses in the placentas of pregnant patients recovered from COVID-19. Cell Proliferation, e13091. https://doi.org/10.1111/cpr.13091
Wu H, et al. Molecular Evidence Suggesting the Persistence of Residual SARS-CoV-2 and Immune Responses in the Placentas of Pregnant Patients Recovered From COVID-19. Cell Prolif. 2021 Jul 22;e13091. PubMed PMID: 34291856.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular evidence suggesting the persistence of residual SARS-CoV-2 and immune responses in the placentas of pregnant patients recovered from COVID-19. AU - Wu,Hao, AU - Liao,Shujie, AU - Wang,Yiming, AU - Guo,Ming, AU - Lin,Xingguang, AU - Wu,Jianli, AU - Wang,Renjie, AU - Lv,Dan, AU - Wu,Di, AU - He,Mengzhou, AU - Hu,Bai, AU - Long,Rui, AU - Peng,Jing, AU - Yang,Hui, AU - Yin,Heng, AU - Wang,Xin, AU - Huang,Zhixiang, AU - Lan,Ke, AU - Zhou,Yanbin, AU - Zhang,Wei, AU - Xiao,Zhenyu, AU - Zhao,Yun, AU - Deng,Dongrui, AU - Wang,Hongmei, Y1 - 2021/07/22/ PY - 2021/06/04/revised PY - 2021/04/20/received PY - 2021/06/18/accepted PY - 2021/7/22/entrez PY - 2021/7/23/pubmed PY - 2021/7/23/medline KW - COVID-19 KW - SARS-CoV-2 KW - cytokine KW - immune response KW - placenta KW - pregnant women SP - e13091 EP - e13091 JF - Cell proliferation JO - Cell Prolif N2 - OBJECTIVES: Recent studies have shown the presence of SARS-CoV-2 in the tissues of clinically recovered patients and persistent immune symptoms in discharged patients for up to several months. Pregnant patients were shown to be a high-risk group for COVID-19. Based on these findings, we assessed SARS-CoV-2 nucleic acid and protein retention in the placentas of pregnant women who had fully recovered from COVID-19 and cytokine fluctuations in maternal and foetal tissues. MATERIALS AND METHODS: Remnant SARS-CoV-2 in the term placenta was detected using nucleic acid amplification and immunohistochemical staining of the SARS-CoV-2 protein. The infiltration of CD14+ macrophages into the placental villi was detected by immunostaining. The cytokines in the placenta, maternal plasma, neonatal umbilical cord, cord blood and amniotic fluid specimens at delivery were profiled using the Luminex assay. RESULTS: Residual SARS-CoV-2 nucleic acid and protein were detected in the term placentas of recovered pregnant women. The infiltration of CD14+ macrophages into the placental villi of the recovered pregnant women was higher than that in the controls. Furthermore, the cytokine levels in the placenta, maternal plasma, neonatal umbilical cord, cord blood and amniotic fluid specimens fluctuated significantly. CONCLUSIONS: Our study showed that SARS-CoV-2 nucleic acid (in one patient) and protein (in five patients) were present in the placentas of clinically recovered pregnant patients for more than 3 months after diagnosis. The immune responses induced by the virus may lead to prolonged and persistent symptoms in the maternal plasma, placenta, umbilical cord, cord blood and amniotic fluid. SN - 1365-2184 UR - https://www.unboundmedicine.com/medline/citation/34291856/Molecular_evidence_suggesting_the_persistence_of_residual_SARS-CoV-2_and_immune_responses_in_the_placentas_of_pregnant_patients_recovered_from_COVID-19. L2 - https://doi.org/10.1111/cpr.13091 DB - PRIME DP - Unbound Medicine ER -
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