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Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family during Development and Stress.
Int J Mol Sci. 2021 Jul 19; 22(14)IJ

Abstract

Adenylate kinase (ADK) is widely distributed in organisms and plays an important role in cellular energy homeostasis. In plants, ADK has important functions in plant growth and development regulation as well as in adaptation to the environment. However, little information is available about the ADK genes in tomato (Solanum lycopersicum), an important economic crop. To investigate the characteristics and functions of ADK genes in tomato, a total of 11 ADK genes were identified and named according to their chromosomal locations. The ADK family in Arabidopsis, tomato, potato, and rice was divided into six groups, and motif analysis revealed that each SlADK protein contained five to eight conserved motifs. A total of 4 to 19 exons were identified in tomato ADK gene family members, and interestingly, most members possessed 4 exons. Several stress response elements were identified in the promoter regions of SlADKs. The 11 SlADKs were randomly distributed on 9 of the 12 tomato chromosomes. Three duplication events were observed between tomato chromosomes, and a high degree of conservation of synteny was demonstrated between tomato and potato. The online TomExpress platform prediction revealed that SlADKs were expressed in various tissues and organs, basically consistent with the data obtained from real-time quantitative PCR (qPCR). The qPCR verification was also performed to determine the expression level of SlADKs and demonstrated that the genes responded to multiple abiotic stresses, such as drought, salt, and cold. Besides, the qPCR results showed that SlADK transcription was responsive to most of the applied hormone treatment. For correlation network analysis under 44 global conditions, the results showed that the number of 17, 3, 4, and 6 coexpressed genes matched with SlADK5, 8, 9, and 11, respectively. For specific gene function analysis, expression of SlADK10 was inhibited using virus-induced gene silencing (VIGS). Compared to wild-type plants, plants with silenced SlADK10 gene had poor drought resistance, indicating SlADK10 regulated drought tolerance of tomato positively. In summary, the information provided in the present study will be helpful to understand the evolutionary relationship and their roles of tomato ADK gene family in further research.

Authors+Show Affiliations

Key Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 241000, China.Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing 400044, China.Key Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 241000, China.Key Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 241000, China.Key Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 241000, China.Key Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 241000, China.Key Laboratory for the Conservation and Utilization of Important Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 241000, China.Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing 400044, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34299327

Citation

Yang, Lu, et al. "Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family During Development and Stress." International Journal of Molecular Sciences, vol. 22, no. 14, 2021.
Yang L, Cao H, Zhang X, et al. Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family during Development and Stress. Int J Mol Sci. 2021;22(14).
Yang, L., Cao, H., Zhang, X., Gui, L., Chen, Q., Qian, G., Xiao, J., & Li, Z. (2021). Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family during Development and Stress. International Journal of Molecular Sciences, 22(14). https://doi.org/10.3390/ijms22147708
Yang L, et al. Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family During Development and Stress. Int J Mol Sci. 2021 Jul 19;22(14) PubMed PMID: 34299327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-Wide Identification and Expression Analysis of Tomato ADK Gene Family during Development and Stress. AU - Yang,Lu, AU - Cao,Haohao, AU - Zhang,Xiaoping, AU - Gui,Liangxian, AU - Chen,Qiang, AU - Qian,Gui, AU - Xiao,Jiaxin, AU - Li,Zhengguo, Y1 - 2021/07/19/ PY - 2021/06/19/received PY - 2021/07/14/revised PY - 2021/07/15/accepted PY - 2021/7/24/entrez PY - 2021/7/25/pubmed PY - 2021/8/12/medline KW - ADK gene family KW - genome-wide KW - stress KW - tomato JF - International journal of molecular sciences JO - Int J Mol Sci VL - 22 IS - 14 N2 - Adenylate kinase (ADK) is widely distributed in organisms and plays an important role in cellular energy homeostasis. In plants, ADK has important functions in plant growth and development regulation as well as in adaptation to the environment. However, little information is available about the ADK genes in tomato (Solanum lycopersicum), an important economic crop. To investigate the characteristics and functions of ADK genes in tomato, a total of 11 ADK genes were identified and named according to their chromosomal locations. The ADK family in Arabidopsis, tomato, potato, and rice was divided into six groups, and motif analysis revealed that each SlADK protein contained five to eight conserved motifs. A total of 4 to 19 exons were identified in tomato ADK gene family members, and interestingly, most members possessed 4 exons. Several stress response elements were identified in the promoter regions of SlADKs. The 11 SlADKs were randomly distributed on 9 of the 12 tomato chromosomes. Three duplication events were observed between tomato chromosomes, and a high degree of conservation of synteny was demonstrated between tomato and potato. The online TomExpress platform prediction revealed that SlADKs were expressed in various tissues and organs, basically consistent with the data obtained from real-time quantitative PCR (qPCR). The qPCR verification was also performed to determine the expression level of SlADKs and demonstrated that the genes responded to multiple abiotic stresses, such as drought, salt, and cold. Besides, the qPCR results showed that SlADK transcription was responsive to most of the applied hormone treatment. For correlation network analysis under 44 global conditions, the results showed that the number of 17, 3, 4, and 6 coexpressed genes matched with SlADK5, 8, 9, and 11, respectively. For specific gene function analysis, expression of SlADK10 was inhibited using virus-induced gene silencing (VIGS). Compared to wild-type plants, plants with silenced SlADK10 gene had poor drought resistance, indicating SlADK10 regulated drought tolerance of tomato positively. In summary, the information provided in the present study will be helpful to understand the evolutionary relationship and their roles of tomato ADK gene family in further research. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/34299327/Genome_Wide_Identification_and_Expression_Analysis_of_Tomato_ADK_Gene_Family_during_Development_and_Stress_ L2 - https://www.mdpi.com/resolver?pii=ijms22147708 DB - PRIME DP - Unbound Medicine ER -