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Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars.
Int J Mol Sci. 2021 Jul 19; 22(14)IJ

Abstract

The low-molecular weight glutenin subunit (LMW-GS) composition of wheat (Triticum aestivum) flour has important effects on end-use quality. However, assessing the contributions of each LMW-GS to flour quality remains challenging because of the complex LMW-GS composition and allelic variation among wheat cultivars. Therefore, accurate and reliable determination of LMW-GS alleles in germplasm remains an important challenge for wheat breeding. In this study, we used an optimized reversed-phase HPLC method and proteomics approach comprising 2-D gels coupled with liquid chromatography-tandem mass spectrometry (MS/MS) to discriminate individual LMW-GSs corresponding to alleles encoded by the Glu-A3, Glu-B3, and Glu-D3 loci in the 'Aroona' cultivar and 12 'Aroona' near-isogenic lines (ARILs), which contain unique LMW-GS alleles in the same genetic background. The LMW-GS separation patterns for 'Aroona' and ARILs on chromatograms and 2-D gels were consistent with those from a set of 10 standard wheat cultivars for Glu-3. Furthermore, 12 previously uncharacterized spots in 'Aroona' and ARILs were excised from 2-D gels, digested with chymotrypsin, and subjected to MS/MS. We identified their gene haplotypes and created a 2-D gel map of LMW-GS alleles in the germplasm for breeding and screening for desirable LMW-GS alleles for wheat quality improvement.

Authors+Show Affiliations

Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea.National Institute of Agricultural Science, RDA, Jeonju 54874, Korea.Division of Horticultural Biotechnology, Hankyong National University, Anseong 17579, Korea.USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA. Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, 20146 Hamburg, Germany.National Institute of Agricultural Science, RDA, Jeonju 54874, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34299329

Citation

Cho, Kyoungwon, et al. "Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars." International Journal of Molecular Sciences, vol. 22, no. 14, 2021.
Cho K, Jang YR, Lim SH, et al. Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars. Int J Mol Sci. 2021;22(14).
Cho, K., Jang, Y. R., Lim, S. H., Altenbach, S. B., Gu, Y. Q., Simon-Buss, A., & Lee, J. Y. (2021). Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars. International Journal of Molecular Sciences, 22(14). https://doi.org/10.3390/ijms22147709
Cho K, et al. Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars. Int J Mol Sci. 2021 Jul 19;22(14) PubMed PMID: 34299329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars. AU - Cho,Kyoungwon, AU - Jang,You-Ran, AU - Lim,Sun-Hyung, AU - Altenbach,Susan B, AU - Gu,Yong Q, AU - Simon-Buss,Annamaria, AU - Lee,Jong-Yeol, Y1 - 2021/07/19/ PY - 2021/06/24/received PY - 2021/07/13/revised PY - 2021/07/15/accepted PY - 2021/7/24/entrez PY - 2021/7/25/pubmed PY - 2021/8/12/medline KW - Aroona near-isogenic lines KW - LMW-GS KW - RP-HPLC KW - proteomic analysis KW - standard wheat cultivars JF - International journal of molecular sciences JO - Int J Mol Sci VL - 22 IS - 14 N2 - The low-molecular weight glutenin subunit (LMW-GS) composition of wheat (Triticum aestivum) flour has important effects on end-use quality. However, assessing the contributions of each LMW-GS to flour quality remains challenging because of the complex LMW-GS composition and allelic variation among wheat cultivars. Therefore, accurate and reliable determination of LMW-GS alleles in germplasm remains an important challenge for wheat breeding. In this study, we used an optimized reversed-phase HPLC method and proteomics approach comprising 2-D gels coupled with liquid chromatography-tandem mass spectrometry (MS/MS) to discriminate individual LMW-GSs corresponding to alleles encoded by the Glu-A3, Glu-B3, and Glu-D3 loci in the 'Aroona' cultivar and 12 'Aroona' near-isogenic lines (ARILs), which contain unique LMW-GS alleles in the same genetic background. The LMW-GS separation patterns for 'Aroona' and ARILs on chromatograms and 2-D gels were consistent with those from a set of 10 standard wheat cultivars for Glu-3. Furthermore, 12 previously uncharacterized spots in 'Aroona' and ARILs were excised from 2-D gels, digested with chymotrypsin, and subjected to MS/MS. We identified their gene haplotypes and created a 2-D gel map of LMW-GS alleles in the germplasm for breeding and screening for desirable LMW-GS alleles for wheat quality improvement. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/34299329/Proteomic_Determination_of_Low_Molecular_Weight_Glutenin_Subunit_Composition_in_Aroona_Near_Isogenic_Lines_and_Standard_Wheat_Cultivars_ L2 - https://www.mdpi.com/resolver?pii=ijms22147709 DB - PRIME DP - Unbound Medicine ER -