Tags

Type your tag names separated by a space and hit enter

Cross-reactivity of some Micrurus venoms against experimental and therapeutic anti-Micrurus antivenoms.
Toxicon. 2021 09; 200:153-164.T

Abstract

We developed experimental equine polyvalent and monovalent antivenoms against the venoms of Micrurus (M.) fulvius, M. nigrocinctus and M. surinamensis and studied their immunochemical reactivity on the venoms used as immunogens and on M. pyrrhocryptus, M altirostris and M. balyocoriphus venoms. Assessment of the neutralizing capacity of the polyvalent experimental antivenom was based on inhibition of lethality (preincubation and rescue assay experiments in mice) and indirect hemolytic and phospholipase activities. The immunochemical reactivity and neutralizing capacity were compared with those of two therapeutic antivenoms used for the treatment of coral snake envenomation in North America and in Argentina. In general, the experimental antivenom conferred a comparable level of neutralization against the venoms used as immunogens when compared to the therapeutic antivenoms and a certain level of cross-neutralization against the other venoms. The results suggest the need for additional venoms in the immunogenic mixture used, in order to obtain a broad spectrum anti-Micrurus antivenom with a good neutralizing potency. Paraspecific neutralization of South American coral snake venoms, although present at a higher level than the neutralization conferred by available nonspecific Micrurus therapeutic antivenoms, was rather low in relation to the specific neutralizing capacity.

Authors+Show Affiliations

Instituto Nacional de Producción de Biológicos, ANLIS "Dr. Carlos G. Malbrán", Ministerio de Salud, Buenos Aires, Argentina; Primera Cátedra de Toxicología, Facultad de Medicina, Universidad de Buenos Aires, Argentina. Electronic address: aderoodt@gmail.com.Instituto Nacional de Producción de Biológicos, ANLIS "Dr. Carlos G. Malbrán", Ministerio de Salud, Buenos Aires, Argentina.Instituto de Diagnóstico y Referencia Epidemiológico INDRE, México DF, Mexico.Cátedra de Inmunología, Facultad de Ciencias Veterinarias de la Universidad Nacional de La Plata, Argentina.Cátedra de Inmunología, Facultad de Ciencias Veterinarias de la Universidad Nacional de La Plata, Argentina.Instituto Nacional de Producción de Biológicos, ANLIS "Dr. Carlos G. Malbrán", Ministerio de Salud, Buenos Aires, Argentina.Instituto Nacional de Producción de Biológicos, ANLIS "Dr. Carlos G. Malbrán", Ministerio de Salud, Buenos Aires, Argentina.Instituto Nacional de Producción de Biológicos, ANLIS "Dr. Carlos G. Malbrán", Ministerio de Salud, Buenos Aires, Argentina.Instituto Nacional de Producción de Biológicos, ANLIS "Dr. Carlos G. Malbrán", Ministerio de Salud, Buenos Aires, Argentina.Instituto Nacional de Producción de Biológicos, ANLIS "Dr. Carlos G. Malbrán", Ministerio de Salud, Buenos Aires, Argentina.Primera Cátedra de Toxicología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.Instituto de Biotecnología de la Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34303716

Citation

de Roodt, Adolfo Rafael, et al. "Cross-reactivity of some Micrurus Venoms Against Experimental and Therapeutic anti-Micrurus Antivenoms." Toxicon : Official Journal of the International Society On Toxinology, vol. 200, 2021, pp. 153-164.
de Roodt AR, Lanari LC, Ramírez JE, et al. Cross-reactivity of some Micrurus venoms against experimental and therapeutic anti-Micrurus antivenoms. Toxicon. 2021;200:153-164.
de Roodt, A. R., Lanari, L. C., Ramírez, J. E., Gómez, C., Barragán, J., Litwin, S., Henriët van Grootheest, J., Desio, M., Dokmetjian, J. C., Dolab, J. A., Damin, C. F., & Alagón, A. (2021). Cross-reactivity of some Micrurus venoms against experimental and therapeutic anti-Micrurus antivenoms. Toxicon : Official Journal of the International Society On Toxinology, 200, 153-164. https://doi.org/10.1016/j.toxicon.2021.07.011
de Roodt AR, et al. Cross-reactivity of some Micrurus Venoms Against Experimental and Therapeutic anti-Micrurus Antivenoms. Toxicon. 2021;200:153-164. PubMed PMID: 34303716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cross-reactivity of some Micrurus venoms against experimental and therapeutic anti-Micrurus antivenoms. AU - de Roodt,Adolfo Rafael, AU - Lanari,Laura Cecilia, AU - Ramírez,Judith Estévez, AU - Gómez,Carlos, AU - Barragán,Javier, AU - Litwin,Silvana, AU - Henriët van Grootheest,Jantine, AU - Desio,Marcela, AU - Dokmetjian,José Christian, AU - Dolab,Jorge Adrián, AU - Damin,Carlos Fabián, AU - Alagón,Alejandro, Y1 - 2021/07/23/ PY - 2021/04/15/received PY - 2021/06/25/revised PY - 2021/07/15/accepted PY - 2021/7/26/pubmed PY - 2021/9/8/medline PY - 2021/7/25/entrez KW - Antivenom KW - Coral snakes KW - Envenomation KW - Immunization KW - Micrurus KW - Therapeutic SP - 153 EP - 164 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 200 N2 - We developed experimental equine polyvalent and monovalent antivenoms against the venoms of Micrurus (M.) fulvius, M. nigrocinctus and M. surinamensis and studied their immunochemical reactivity on the venoms used as immunogens and on M. pyrrhocryptus, M altirostris and M. balyocoriphus venoms. Assessment of the neutralizing capacity of the polyvalent experimental antivenom was based on inhibition of lethality (preincubation and rescue assay experiments in mice) and indirect hemolytic and phospholipase activities. The immunochemical reactivity and neutralizing capacity were compared with those of two therapeutic antivenoms used for the treatment of coral snake envenomation in North America and in Argentina. In general, the experimental antivenom conferred a comparable level of neutralization against the venoms used as immunogens when compared to the therapeutic antivenoms and a certain level of cross-neutralization against the other venoms. The results suggest the need for additional venoms in the immunogenic mixture used, in order to obtain a broad spectrum anti-Micrurus antivenom with a good neutralizing potency. Paraspecific neutralization of South American coral snake venoms, although present at a higher level than the neutralization conferred by available nonspecific Micrurus therapeutic antivenoms, was rather low in relation to the specific neutralizing capacity. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/34303716/Cross_reactivity_of_some_Micrurus_venoms_against_experimental_and_therapeutic_anti_Micrurus_antivenoms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(21)00207-5 DB - PRIME DP - Unbound Medicine ER -