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Evaluation and comparison of conjunctival swab polymerase chain reaction results in SARS-CoV-2 patients with and without ocular manifestations.
Indian J Ophthalmol. 2021 Aug; 69(8):2211-2214.IJ

Abstract

Purpose

The aim of this study was to evaluate and compare the presence of SARS-CoV-2 in tears of patients with and without ocular symptoms in SARS-CoV-2 positive patients.

Methods

The prospective observational study conducted on 60 consecutive SARS-CoV-2 positive patients with ocular complaints was compared with 60 controls who had no ocular manifestations. The tear samples were taken within 48 h of admission from both the eyes of the enrolled patients for evaluating the presence SARS-CoV-2 by reverse transcription-polymerase chain reaction.

Results

Eleven cases (18.33%) tested positive for SARS-CoV-2 in tears on RT-PCR from cojunctival swab compared to 10 (16.66%) controls. The difference was not statistical significant. The difference between mean age of patients who tested positive or negative was also without statistical significance (P = 0.652), but the difference between patients who tested positive or negative by conjunctival swab for SARS-CoV-2 was statistically significant in terms of severity of COVID-19 disease (P = 0.0011), presence of comorbidity (P = 0.0015), mean TLC (P = 0.00498), and mean d dimer (P = 0.00465).

Conclusion

Though the percentage of patients with positive RT PCR from conjunctival secretions is significantly less than nasopharyngeal swabs, potential risk of transmission of SARS-Co-2 through tears cannot be ruled out. Moreover, SARS-CoV-2 can be present in tears irrespective of ocular involvement.

Authors+Show Affiliations

Department of Ophthalmology, Regional Institute of Ophthalmology, GMC, Amritsar, India.Department of Ophthalmology, Regional Institute of Ophthalmology, GMC, Amritsar, India.Department of Microbiology, GMC, Amritsar, Punjab, India.Department of Microbiology, GMC, Amritsar, Punjab, India.Department of Ophthalmology, Regional Institute of Ophthalmology, GMC, Amritsar, India.

Pub Type(s)

Journal Article
Observational Study

Language

eng

PubMed ID

34304212

Citation

Kaur, Prempal, et al. "Evaluation and Comparison of Conjunctival Swab Polymerase Chain Reaction Results in SARS-CoV-2 Patients With and Without Ocular Manifestations." Indian Journal of Ophthalmology, vol. 69, no. 8, 2021, pp. 2211-2214.
Kaur P, Sehgal G, Shailpreet , et al. Evaluation and comparison of conjunctival swab polymerase chain reaction results in SARS-CoV-2 patients with and without ocular manifestations. Indian J Ophthalmol. 2021;69(8):2211-2214.
Kaur, P., Sehgal, G., Shailpreet, ., Singh, K. D., & Singh, B. (2021). Evaluation and comparison of conjunctival swab polymerase chain reaction results in SARS-CoV-2 patients with and without ocular manifestations. Indian Journal of Ophthalmology, 69(8), 2211-2214. https://doi.org/10.4103/ijo.IJO_587_21
Kaur P, et al. Evaluation and Comparison of Conjunctival Swab Polymerase Chain Reaction Results in SARS-CoV-2 Patients With and Without Ocular Manifestations. Indian J Ophthalmol. 2021;69(8):2211-2214. PubMed PMID: 34304212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation and comparison of conjunctival swab polymerase chain reaction results in SARS-CoV-2 patients with and without ocular manifestations. AU - Kaur,Prempal, AU - Sehgal,Gaurang, AU - Shailpreet,, AU - Singh,K D, AU - Singh,Bhavkaran, PY - 2021/7/25/entrez PY - 2021/7/26/pubmed PY - 2021/7/31/medline KW - Conjunctival secretions KW - RT PCR KW - S-CoV-2 KW - SAR KW - ocular manifestations SP - 2211 EP - 2214 JF - Indian journal of ophthalmology JO - Indian J Ophthalmol VL - 69 IS - 8 N2 - Purpose: The aim of this study was to evaluate and compare the presence of SARS-CoV-2 in tears of patients with and without ocular symptoms in SARS-CoV-2 positive patients. Methods: The prospective observational study conducted on 60 consecutive SARS-CoV-2 positive patients with ocular complaints was compared with 60 controls who had no ocular manifestations. The tear samples were taken within 48 h of admission from both the eyes of the enrolled patients for evaluating the presence SARS-CoV-2 by reverse transcription-polymerase chain reaction. Results: Eleven cases (18.33%) tested positive for SARS-CoV-2 in tears on RT-PCR from cojunctival swab compared to 10 (16.66%) controls. The difference was not statistical significant. The difference between mean age of patients who tested positive or negative was also without statistical significance (P = 0.652), but the difference between patients who tested positive or negative by conjunctival swab for SARS-CoV-2 was statistically significant in terms of severity of COVID-19 disease (P = 0.0011), presence of comorbidity (P = 0.0015), mean TLC (P = 0.00498), and mean d dimer (P = 0.00465). Conclusion: Though the percentage of patients with positive RT PCR from conjunctival secretions is significantly less than nasopharyngeal swabs, potential risk of transmission of SARS-Co-2 through tears cannot be ruled out. Moreover, SARS-CoV-2 can be present in tears irrespective of ocular involvement. SN - 1998-3689 UR - https://www.unboundmedicine.com/medline/citation/34304212/Evaluation_and_comparison_of_conjunctival_swab_polymerase_chain_reaction_results_in_SARS_CoV_2_patients_with_and_without_ocular_manifestations_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=34304212.ui DB - PRIME DP - Unbound Medicine ER -