Amplified electrochemical antibiotic aptasensing based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework.
Mikrochim Acta. 2021 08 04; 188(8):286.MA

Abstract

A facile and versatile competitive electrochemical aptasensor for tobramycin (TOB) detection is described using electrochemical-deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework (AuNPs/P-MOF) as signal-amplification platform and a DNA probe labeled with methylene blue (MB) at the 3'-end (MB-Probe) as a signal producer. First, F-Probe (short complementary DNA strands of both the aptamer and the MB-Probe label with a sulfhydryl group at the 5'-end) was immobilized on the AuNPs/P-MOF modified electrode as detection probes, which competed with TOB in binding to the aptamer. TOB-aptamer binding resulted in F-Probe remaining unhybridized on the electrode surface, so that a significant current response was generated by hybridizing with MB-Probe instead. The developed strategy showed favorable repeatability, with a relative standard deviation (RSD) of 4.3% computed over five independent assays, and high stability, with only 6.8% degradation after 15 days of storage. Under optimal conditions, the proposed aptamer strategy exhibited a linear detection range from 100 pM to 500 nM with a limit of detection (LOD) of 56 pM (S/N = 3). The electrochemical aptasensor demonstrated remarkable selectivity, and its feasibility for accurate and quantitative detection of TOB in milk samples was confirmed (RSD < 4.5%). Due to its simple design, easy operation, and high sensitivity and selectivity, the proposed method could expect to detect other antibiotics by replacing the aptamers. In summary, this study provides a simple and effective new strategy for electrochemical aptasening based on MOF-based sensing interface. Scheme illustration of label-free competitive electrochemical aptamer-based detection of tobramycin based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework as signal-amplification platform.

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Authors+Show Affiliations

Zhang Y

Li B

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People's Republic of China. College of Food Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China.

College of Food Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China.

Xue L

Ding Y

Department of Food Science & Technology, Institute of Food Safety and Nutrition, Jinan University, Guangzhou, 510632, People's Republic of China. dingyu@jnu.edu.cn.

Wu Q

MeSH

AnimalsAnti-Bacterial AgentsAptamers, NucleotideBiosensing TechniquesDNAElectrochemical TechniquesFood ContaminationGoldImmobilized Nucleic AcidsIronLimit of DetectionMetal NanoparticlesMetal-Organic FrameworksMethylene BlueMilkOxidation-ReductionPolyethyleneimineReproducibility of ResultsTobramycin

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34345968

Citation

Zhang, Youxiong, et al. "Amplified Electrochemical Antibiotic Aptasensing Based On Electrochemically Deposited AuNPs Coordinated With PEI-functionalized Fe-based Metal-organic Framework." Mikrochimica Acta, vol. 188, no. 8, 2021, p. 286.

Zhang Y, Li B, Wei X, et al. Amplified electrochemical antibiotic aptasensing based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework. Mikrochim Acta. 2021;188(8):286.

Zhang, Y., Li, B., Wei, X., Gu, Q., Chen, M., Zhang, J., Mo, S., Wang, J., Xue, L., Ding, Y., & Wu, Q. (2021). Amplified electrochemical antibiotic aptasensing based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework. Mikrochimica Acta, 188(8), 286. https://doi.org/10.1007/s00604-021-04912-z

Zhang Y, et al. Amplified Electrochemical Antibiotic Aptasensing Based On Electrochemically Deposited AuNPs Coordinated With PEI-functionalized Fe-based Metal-organic Framework. Mikrochim Acta. 2021 08 4;188(8):286. PubMed PMID: 34345968.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Amplified electrochemical antibiotic aptasensing based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework. AU - Zhang,Youxiong, AU - Li,Bing, AU - Wei,Xianhu, AU - Gu,Qihui, AU - Chen,Moutong, AU - Zhang,Jumei, AU - Mo,Shuping, AU - Wang,Juan, AU - Xue,Liang, AU - Ding,Yu, AU - Wu,Qingping, Y1 - 2021/08/04/ PY - 2021/03/30/received PY - 2021/06/22/accepted PY - 2021/8/4/entrez PY - 2021/8/5/pubmed PY - 2022/1/4/medline KW - Aptasensor KW - AuNPs KW - Electrochemical sensing KW - Food contamination KW - Tobramycin KW - Voltammetry SP - 286 EP - 286 JF - Mikrochimica acta JO - Mikrochim Acta VL - 188 IS - 8 N2 - A facile and versatile competitive electrochemical aptasensor for tobramycin (TOB) detection is described using electrochemical-deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework (AuNPs/P-MOF) as signal-amplification platform and a DNA probe labeled with methylene blue (MB) at the 3'-end (MB-Probe) as a signal producer. First, F-Probe (short complementary DNA strands of both the aptamer and the MB-Probe label with a sulfhydryl group at the 5'-end) was immobilized on the AuNPs/P-MOF modified electrode as detection probes, which competed with TOB in binding to the aptamer. TOB-aptamer binding resulted in F-Probe remaining unhybridized on the electrode surface, so that a significant current response was generated by hybridizing with MB-Probe instead. The developed strategy showed favorable repeatability, with a relative standard deviation (RSD) of 4.3% computed over five independent assays, and high stability, with only 6.8% degradation after 15 days of storage. Under optimal conditions, the proposed aptamer strategy exhibited a linear detection range from 100 pM to 500 nM with a limit of detection (LOD) of 56 pM (S/N = 3). The electrochemical aptasensor demonstrated remarkable selectivity, and its feasibility for accurate and quantitative detection of TOB in milk samples was confirmed (RSD < 4.5%). Due to its simple design, easy operation, and high sensitivity and selectivity, the proposed method could expect to detect other antibiotics by replacing the aptamers. In summary, this study provides a simple and effective new strategy for electrochemical aptasening based on MOF-based sensing interface. Scheme illustration of label-free competitive electrochemical aptamer-based detection of tobramycin based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework as signal-amplification platform. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/34345968/Amplified_electrochemical_antibiotic_aptasensing_based_on_electrochemically_deposited_AuNPs_coordinated_with_PEI_functionalized_Fe_based_metal_organic_framework_ DB - PRIME DP - Unbound Medicine ER -

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Amplified electrochemical antibiotic aptasensing based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework.Mikrochim Acta. 2021 08 04; 188(8):286.MA