Setup of an Ultrasonic-Assisted Extraction to Obtain High Phenolic Recovery in Crataegus monogyna Leaves.
Molecules. 2021 Jul 27; 26(15)M

Abstract

Hawthorn leaves are a rich source of phenolic compounds that possess beneficial activities for human health. Ultrasonic-assisted extraction (UAE) is an extraction technique frequently used for the isolation of phenolic compounds in plants. Thus, in this study, a Box-Behnken design was used to optimize UAE conditions such as the percentage of acetone, the extraction time and solvent-to-solid ratio (v/w) in order to obtain the maximum content of total compounds by Folin-Ciocalteu and the maximum in vitro antioxidant activity by DPPH, ABTS and FRAP assays in Crataegus monogyna leaves. The optimum conditions to obtain the highest total phenolic content and antioxidant activities were 50% acetone, 55 min and 1/1000 (w/v). A total of 30 phenolic compounds were identified and quantified in C. monogyna leaf extract obtained at these optimum UAE conditions. HPLC-MS allows the identification and quantification of 19 phenolic compounds and NP-HPLC-FLD analyses showed the presence of 11 proanthocyanidins. According to the results, the most concentrated phenolic compounds in C. monogyna leaf extract obtained at optimum UAE conditions were phenolic acid derivatives such as protocatechuic acid-glucoside, dihydroxy benzoic acid pentoside and chlorogenic acid, flavones such as 2″-O-rhamnosyl-C-hexosyl-apigenin, flavonols such as hyperoside and isoquercetin and proanthocyanidins such as monomer and dimer. As a result, the optimized UAE conditions could be used to obtain an extract of C. monogyna leaves enriched with phenolic compounds.

Links

PMC Free PDF

Authors+Show Affiliations

Martín-García B

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain. Department of Nutrition and Food Science, University of Granada, Campus of Cartuja, 18071 Granada, Spain. Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, University of Granada, Avda del Conocimiento s/n., 18100 Granada, Spain.

Razola-Díaz MDC

Department of Nutrition and Food Science, University of Granada, Campus of Cartuja, 18071 Granada, Spain. Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, University of Granada, Avda del Conocimiento s/n., 18100 Granada, Spain.

Gómez-Caravaca AM

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain.

Benítez G

Department of Botany, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.

Verardo V

MeSH

AcetoneAntioxidantsChromatography, High Pressure LiquidCrataegusLiquid-Liquid ExtractionMass SpectrometryPhenolsPlant LeavesProanthocyanidinsSolventsUltrasonic WavesWater

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34361687

Citation

Martín-García, Beatriz, et al. "Setup of an Ultrasonic-Assisted Extraction to Obtain High Phenolic Recovery in Crataegus Monogyna Leaves." Molecules (Basel, Switzerland), vol. 26, no. 15, 2021.

Martín-García B, Razola-Díaz MDC, Gómez-Caravaca AM, et al. Setup of an Ultrasonic-Assisted Extraction to Obtain High Phenolic Recovery in Crataegus monogyna Leaves. Molecules. 2021;26(15).

Martín-García, B., Razola-Díaz, M. D. C., Gómez-Caravaca, A. M., Benítez, G., & Verardo, V. (2021). Setup of an Ultrasonic-Assisted Extraction to Obtain High Phenolic Recovery in Crataegus monogyna Leaves. Molecules (Basel, Switzerland), 26(15). https://doi.org/10.3390/molecules26154536

Martín-García B, et al. Setup of an Ultrasonic-Assisted Extraction to Obtain High Phenolic Recovery in Crataegus Monogyna Leaves. Molecules. 2021 Jul 27;26(15) PubMed PMID: 34361687.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Setup of an Ultrasonic-Assisted Extraction to Obtain High Phenolic Recovery in Crataegus monogyna Leaves. AU - Martín-García,Beatriz, AU - Razola-Díaz,María Del Carmen, AU - Gómez-Caravaca,Ana María, AU - Benítez,Guillermo, AU - Verardo,Vito, Y1 - 2021/07/27/ PY - 2021/06/30/received PY - 2021/07/21/revised PY - 2021/07/23/accepted PY - 2021/8/7/entrez PY - 2021/8/8/pubmed PY - 2021/9/25/medline KW - Box–Behnken design KW - HPLC-ESI-TOF-MS KW - HPLC-FLD KW - Hawthorn (Crataegus monogyna) KW - ultrasonic-assisted extraction JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 15 N2 - Hawthorn leaves are a rich source of phenolic compounds that possess beneficial activities for human health. Ultrasonic-assisted extraction (UAE) is an extraction technique frequently used for the isolation of phenolic compounds in plants. Thus, in this study, a Box-Behnken design was used to optimize UAE conditions such as the percentage of acetone, the extraction time and solvent-to-solid ratio (v/w) in order to obtain the maximum content of total compounds by Folin-Ciocalteu and the maximum in vitro antioxidant activity by DPPH, ABTS and FRAP assays in Crataegus monogyna leaves. The optimum conditions to obtain the highest total phenolic content and antioxidant activities were 50% acetone, 55 min and 1/1000 (w/v). A total of 30 phenolic compounds were identified and quantified in C. monogyna leaf extract obtained at these optimum UAE conditions. HPLC-MS allows the identification and quantification of 19 phenolic compounds and NP-HPLC-FLD analyses showed the presence of 11 proanthocyanidins. According to the results, the most concentrated phenolic compounds in C. monogyna leaf extract obtained at optimum UAE conditions were phenolic acid derivatives such as protocatechuic acid-glucoside, dihydroxy benzoic acid pentoside and chlorogenic acid, flavones such as 2″-O-rhamnosyl-C-hexosyl-apigenin, flavonols such as hyperoside and isoquercetin and proanthocyanidins such as monomer and dimer. As a result, the optimized UAE conditions could be used to obtain an extract of C. monogyna leaves enriched with phenolic compounds. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/34361687/Setup_of_an_Ultrasonic_Assisted_Extraction_to_Obtain_High_Phenolic_Recovery_in_Crataegus_monogyna_Leaves_ DB - PRIME DP - Unbound Medicine ER -

Tags

Setup of an Ultrasonic-Assisted Extraction to Obtain High Phenolic Recovery in Crataegus monogyna Leaves.Molecules. 2021 Jul 27; 26(15)M